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The map label for this gene is gidB

Identifier: 146337361

GI number: 146337361

Start: 207312

End: 207980

Strand: Reverse

Name: gidB

Synonym: BRADO0194

Alternate gene names: 146337361

Gene position: 207980-207312 (Counterclockwise)

Preceding gene: 146337362

Following gene: 146337360

Centisome position: 2.79

GC content: 61.88

Gene sequence:

>669_bases
GTGAAACCGACATCGTCCGCAGCGGCACGGAATGAGCTCGCATCTGACAAGGCGGCGGCTCTGGCGCTGACTCCCGTTTC
ACGTGAAACCGAGCGGCGCCTCGATCGCTACATCGCGTTGCTGCTGGAGTGGCAGGCCAAGACCAATCTGGTCGCGCCGT
CCACCTTGCCGCATCTCTGGACGCGGCACATTTCGGATTCGCTCCAACTGCTCGATCTCGCGCCCGAGGCCAGCTTCTGG
GTCGACCTCGGCAGCGGCGGCGGCTTCCCCGGCGTCGTGCTCGCTTGCGCGCTCGCCGAACGGCCCGGTGCAAAGGTCCA
TCTGGTGGAGCGCATCGCCAAGAAGGCGGCGTTCCTACGCGAAGCAATTCGCGTCACCGGCTCACCTGGGACAGTGCATT
TGTCAGAGATCGGGGATATCGTGGAGAAGTGGAGCGGGCGAGTCGATTGCGTGACCGCACGCGCGCTGGCTCCGCTACAC
CAACTCGTCGGCTTTGCGGAGCCCTTGGTGAAAAAAGGCGCGAAAGCGCTGTTTCTCAAGGGTCAAGATGTAGATGCGGA
ATTGACCGAATCTACCAAGTATTGGAAAATTGAGCCGAAACTTCATTCCAGCCGGACGGGCGGGCAGGGCTGGATCGTCG
CAATCGATTGCATCGAGCGACGAAGCTGA

Upstream 100 bases:

>100_bases
TCGGATCGATGGGATGACGCCGGCGGCGCTTGGAATTCTTGCGGCGTATCTTCGCCGCGAAGCTCGGCGCAACAAGGCTG
CGAGCTGAGGAACGTTTCAC

Downstream 100 bases:

>100_bases
ATACCCGCAGCAACAACCATGGCGACCGCGCATGTCCGTAATTGATCAGATTTATCAAGAAGATAGGGCTCCGCACCCAG
AGGGGCATCCGCGCATCCTG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKPTSSAAARNELASDKAAALALTPVSRETERRLDRYIALLLEWQAKTNLVAPSTLPHLWTRHISDSLQLLDLAPEASFW
VDLGSGGGFPGVVLACALAERPGAKVHLVERIAKKAAFLREAIRVTGSPGTVHLSEIGDIVEKWSGRVDCVTARALAPLH
QLVGFAEPLVKKGAKALFLKGQDVDAELTESTKYWKIEPKLHSSRTGGQGWIVAIDCIERRS

Sequences:

>Translated_222_residues
MKPTSSAAARNELASDKAAALALTPVSRETERRLDRYIALLLEWQAKTNLVAPSTLPHLWTRHISDSLQLLDLAPEASFW
VDLGSGGGFPGVVLACALAERPGAKVHLVERIAKKAAFLREAIRVTGSPGTVHLSEIGDIVEKWSGRVDCVTARALAPLH
QLVGFAEPLVKKGAKALFLKGQDVDAELTESTKYWKIEPKLHSSRTGGQGWIVAIDCIERRS
>Mature_222_residues
MKPTSSAAARNELASDKAAALALTPVSRETERRLDRYIALLLEWQAKTNLVAPSTLPHLWTRHISDSLQLLDLAPEASFW
VDLGSGGGFPGVVLACALAERPGAKVHLVERIAKKAAFLREAIRVTGSPGTVHLSEIGDIVEKWSGRVDCVTARALAPLH
QLVGFAEPLVKKGAKALFLKGQDVDAELTESTKYWKIEPKLHSSRTGGQGWIVAIDCIERRS

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_BRASO (A4YJT3)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202409.1
- ProteinModelPortal:   A4YJT3
- SMR:   A4YJT3
- STRING:   A4YJT3
- GeneID:   5115700
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0194
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   GGQGWIV
- ProtClustDB:   PRK00107
- BioCyc:   BSP376:BRADO0194-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 24090; Mature: 24090

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTSSAAARNELASDKAAALALTPVSRETERRLDRYIALLLEWQAKTNLVAPSTLPHLW
CCCCCHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
TRHISDSLQLLDLAPEASFWVDLGSGGGFPGVVLACALAERPGAKVHLVERIAKKAAFLR
HHHHHHHHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAIRVTGSPGTVHLSEIGDIVEKWSGRVDCVTARALAPLHQLVGFAEPLVKKGAKALFLK
HHHHHCCCCCEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GQDVDAELTESTKYWKIEPKLHSSRTGGQGWIVAIDCIERRS
CCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKPTSSAAARNELASDKAAALALTPVSRETERRLDRYIALLLEWQAKTNLVAPSTLPHLW
CCCCCHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
TRHISDSLQLLDLAPEASFWVDLGSGGGFPGVVLACALAERPGAKVHLVERIAKKAAFLR
HHHHHHHHHEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
EAIRVTGSPGTVHLSEIGDIVEKWSGRVDCVTARALAPLHQLVGFAEPLVKKGAKALFLK
HHHHHCCCCCEEEHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GQDVDAELTESTKYWKIEPKLHSSRTGGQGWIVAIDCIERRS
CCCCCCHHHCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA