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The map label for this gene is trpA

Identifier: 146337263

GI number: 146337263

Start: 106668

End: 107504

Strand: Reverse

Name: trpA

Synonym: BRADO0090

Alternate gene names: 146337263

Gene position: 107504-106668 (Counterclockwise)

Preceding gene: 146337264

Following gene: 146337262

Centisome position: 1.44

GC content: 68.94

Gene sequence:

>837_bases
GTGACCAGCCGGATGGATGCGCGCTTCGCGCAGTTAAAGAGTGAAGGCCGTTCGGCATTCGTGACGTTCGTGATGGGCGG
CGACCCCGATCCGGAGACGTCGCTCGCGATCGTCAAGGCGCTGCCGCAGGCGGGCGCCGACATCATCGAGATCGGCATGC
CGTTCACCGATCCGATGGCCGACGGCCCGTCGATCCAGGCCGCGGGCCTGCGCGCGCTGAAAGCGGGCATGACCCTGAGG
AAGACGCTGCAGCTGGTGCGCAGTTTCCGCGAGGGCGACGCAGCCACGCCGATCGTGCTGATGGGCTACTACAACCCGAT
CTACATCTATGGCGTCGACAAGTTCCTCGACGACGCCAAGAGCGCCGGTGTCGACGGCCTGATCATCGTCGACCTGCCGC
CGGAAGAAGACGACGAGCTGTGCATTCCGGCGCTCAAGGCCGGGCTCAACTTCATCCGTCTGGCGACCCCGACCACCGAT
GACAAGCGGCTGCCGGCCGTGCTCGCGAACACCTCCGGCTTCGTCTACTACGTCTCGATCACCGGCATCACCGGTGCGGC
CGCGGCCGACTCCTCCGCCGTCGGGTCGGCCGTTGCGCGAATCAAGCGCCACACGGCGCTGCCGGTCTGCGTCGGTTTCG
GCATTCGGACACCGGAAACCGCCCGGGCTATCGCCGCCCACGCTGACGGCGCCGTGGTCGGCACCGCCCTGGTGGATGCC
CTGAAGGCGAGCCTGGACGCCGAGGGCCGGGCGACGGCCAAGACCGTCAACGCGGTCGCCGACCTTGCCGCCGCCCTCGC
CCAGGGCGTGCGGGGCGCCCAGCAGGCCGCGGAATAG

Upstream 100 bases:

>100_bases
ACAAGCCGAAGGACCATTTGATGGTCGTCAATCTGTGCGGCCGCGGCGACAAGGACGTGCCGCAGGTCGGCGAGATCCTG
CGTAAGAGGGCGAAACAGTC

Downstream 100 bases:

>100_bases
CCATCAACCACCCTTGCATGGAGCGCCGCGGCACGGCTTGCCGTGGCGCTGCCGTCCCGCCATATATCCGGGCGACCGCA
AAACGGAGCTTTTCATGAAC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTSRMDARFAQLKSEGRSAFVTFVMGGDPDPETSLAIVKALPQAGADIIEIGMPFTDPMADGPSIQAAGLRALKAGMTLR
KTLQLVRSFREGDAATPIVLMGYYNPIYIYGVDKFLDDAKSAGVDGLIIVDLPPEEDDELCIPALKAGLNFIRLATPTTD
DKRLPAVLANTSGFVYYVSITGITGAAAADSSAVGSAVARIKRHTALPVCVGFGIRTPETARAIAAHADGAVVGTALVDA
LKASLDAEGRATAKTVNAVADLAAALAQGVRGAQQAAE

Sequences:

>Translated_278_residues
MTSRMDARFAQLKSEGRSAFVTFVMGGDPDPETSLAIVKALPQAGADIIEIGMPFTDPMADGPSIQAAGLRALKAGMTLR
KTLQLVRSFREGDAATPIVLMGYYNPIYIYGVDKFLDDAKSAGVDGLIIVDLPPEEDDELCIPALKAGLNFIRLATPTTD
DKRLPAVLANTSGFVYYVSITGITGAAAADSSAVGSAVARIKRHTALPVCVGFGIRTPETARAIAAHADGAVVGTALVDA
LKASLDAEGRATAKTVNAVADLAAALAQGVRGAQQAAE
>Mature_277_residues
TSRMDARFAQLKSEGRSAFVTFVMGGDPDPETSLAIVKALPQAGADIIEIGMPFTDPMADGPSIQAAGLRALKAGMTLRK
TLQLVRSFREGDAATPIVLMGYYNPIYIYGVDKFLDDAKSAGVDGLIIVDLPPEEDDELCIPALKAGLNFIRLATPTTDD
KRLPAVLANTSGFVYYVSITGITGAAAADSSAVGSAVARIKRHTALPVCVGFGIRTPETARAIAAHADGAVVGTALVDAL
KASLDAEGRATAKTVNAVADLAAALAQGVRGAQQAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=220, Percent_Identity=37.2727272727273, Blast_Score=154, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=41.8410041841004, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BRASO (A4YJI5)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001202311.1
- ProteinModelPortal:   A4YJI5
- SMR:   A4YJI5
- STRING:   A4YJI5
- GeneID:   5115074
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO0090
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   BSP376:BRADO0090-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28746; Mature: 28615

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRMDARFAQLKSEGRSAFVTFVMGGDPDPETSLAIVKALPQAGADIIEIGMPFTDPMA
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPSIQAAGLRALKAGMTLRKTLQLVRSFREGDAATPIVLMGYYNPIYIYGVDKFLDDAK
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
SAGVDGLIIVDLPPEEDDELCIPALKAGLNFIRLATPTTDDKRLPAVLANTSGFVYYVSI
HCCCCEEEEEECCCCCCCCEEHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEEE
TGITGAAAADSSAVGSAVARIKRHTALPVCVGFGIRTPETARAIAAHADGAVVGTALVDA
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHH
LKASLDAEGRATAKTVNAVADLAAALAQGVRGAQQAAE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSRMDARFAQLKSEGRSAFVTFVMGGDPDPETSLAIVKALPQAGADIIEIGMPFTDPMA
CCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPSIQAAGLRALKAGMTLRKTLQLVRSFREGDAATPIVLMGYYNPIYIYGVDKFLDDAK
CCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
SAGVDGLIIVDLPPEEDDELCIPALKAGLNFIRLATPTTDDKRLPAVLANTSGFVYYVSI
HCCCCEEEEEECCCCCCCCEEHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEEE
TGITGAAAADSSAVGSAVARIKRHTALPVCVGFGIRTPETARAIAAHADGAVVGTALVDA
ECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHH
LKASLDAEGRATAKTVNAVADLAAALAQGVRGAQQAAE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA