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The map label for this gene is fabI [H]

Identifier: 146337235

GI number: 146337235

Start: 71097

End: 71903

Strand: Reverse

Name: fabI [H]

Synonym: BRADO0061

Alternate gene names: 146337235

Gene position: 71903-71097 (Counterclockwise)

Preceding gene: 146337236

Following gene: 146337234

Centisome position: 0.96

GC content: 67.04

Gene sequence:

>807_bases
GTGAGCATGCAGGGTCTGATGGCAGGCAAGCGCGGTCTGGTCATGGGCATCGCCAACGACCATTCGATCGCCTGGGGCAT
CGCCAAGACGCTCGCTGCGCACGGCGCCGAGATGGCCTTCACCTATCAGGGCGACGCGCTCGGCAAGCGCGTGCGGCCGC
TCGCCGATGAGCTCGGCGTCAAGCATGTGCTGCCTTGCGACGTCGAGGACATCGCCAGCGTCGACGGCGTCTTCACGAGC
TTGCGCGAGGCCTGGGGCGGGCTCGACTTCATCGTCCACGCCGTCGCCTTCTCCGACAAGAACGAGCTCAAGGGCCGCTA
CGCCGACACCTCGCGCGAGAATTTCTCGCGCACGATGCTGATCTCCTGCTTCTCCTTCACCGAGATCGCCAAGCGCGCCG
CCGAGCTGATGCCGGAGAGCGGCGGCAGCATGATCACGCTGACCTTCGACGGCTCCAACCGCGTGATGCCGAACTACAAC
GTGATGGGCGTCGCCAAGGCCGCGCTCGAAGCCTCGGTGCGCTACCTCGCCGCCGACTACGGCCGCCGCGCGATCCGCGT
CAACGCGATCTCGGCCGGCCCGGTGCGCACGCTCGCGGGTTCCGGCATTGGCGATGCGCGCTTCATGTTCGCGTTCCAGC
AGAAGCACTCGCCGCTCGGCCGCGGCATTACGCTGGAGGAGCTCGGCGGTTCCGCGCTGTATCTGCTGTCCGATCTCTCC
GGCGGCGTCACCGGCGAGATCCACTATGTGGACTCCGGCTACAACGTCATCTCGATGCCGCGGCCCGAGGATCTCAAGGC
GGAGTAG

Upstream 100 bases:

>100_bases
CGGACAACAGCGCGACGCGTTTCGCACCGCTGTCCGGCTATCGACGTCCGGGGAACCTGACGATCATCTTGGCTGGGTCG
GCCCGGCATCACGAAGGAGA

Downstream 100 bases:

>100_bases
TTCCCGCGCGCATCGGTGTGTCCGGCCGCCGTCGGCGGTTCCGGCGGCGCAGCGTGATGCACCCATTTTTTCCCAGTTGA
AGGCCCCGCTCTCCTGCGGA

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSMQGLMAGKRGLVMGIANDHSIAWGIAKTLAAHGAEMAFTYQGDALGKRVRPLADELGVKHVLPCDVEDIASVDGVFTS
LREAWGGLDFIVHAVAFSDKNELKGRYADTSRENFSRTMLISCFSFTEIAKRAAELMPESGGSMITLTFDGSNRVMPNYN
VMGVAKAALEASVRYLAADYGRRAIRVNAISAGPVRTLAGSGIGDARFMFAFQQKHSPLGRGITLEELGGSALYLLSDLS
GGVTGEIHYVDSGYNVISMPRPEDLKAE

Sequences:

>Translated_268_residues
MSMQGLMAGKRGLVMGIANDHSIAWGIAKTLAAHGAEMAFTYQGDALGKRVRPLADELGVKHVLPCDVEDIASVDGVFTS
LREAWGGLDFIVHAVAFSDKNELKGRYADTSRENFSRTMLISCFSFTEIAKRAAELMPESGGSMITLTFDGSNRVMPNYN
VMGVAKAALEASVRYLAADYGRRAIRVNAISAGPVRTLAGSGIGDARFMFAFQQKHSPLGRGITLEELGGSALYLLSDLS
GGVTGEIHYVDSGYNVISMPRPEDLKAE
>Mature_267_residues
SMQGLMAGKRGLVMGIANDHSIAWGIAKTLAAHGAEMAFTYQGDALGKRVRPLADELGVKHVLPCDVEDIASVDGVFTSL
REAWGGLDFIVHAVAFSDKNELKGRYADTSRENFSRTMLISCFSFTEIAKRAAELMPESGGSMITLTFDGSNRVMPNYNV
MGVAKAALEASVRYLAADYGRRAIRVNAISAGPVRTLAGSGIGDARFMFAFQQKHSPLGRGITLEELGGSALYLLSDLSG
GVTGEIHYVDSGYNVISMPRPEDLKAE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=260, Percent_Identity=46.5384615384615, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1789378, Length=264, Percent_Identity=29.1666666666667, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI2367365, Length=254, Percent_Identity=26.3779527559055, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=28.9156626506024, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28732; Mature: 28601

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMQGLMAGKRGLVMGIANDHSIAWGIAKTLAAHGAEMAFTYQGDALGKRVRPLADELGV
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCC
KHVLPCDVEDIASVDGVFTSLREAWGGLDFIVHAVAFSDKNELKGRYADTSRENFSRTML
CEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHH
ISCFSFTEIAKRAAELMPESGGSMITLTFDGSNRVMPNYNVMGVAKAALEASVRYLAADY
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GRRAIRVNAISAGPVRTLAGSGIGDARFMFAFQQKHSPLGRGITLEELGGSALYLLSDLS
CCEEEEEEEECCCCHHHHCCCCCCCHHHHEEEHHHCCCCCCCEEHHHCCCCEEEEEECCC
GGVTGEIHYVDSGYNVISMPRPEDLKAE
CCCEEEEEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure 
SMQGLMAGKRGLVMGIANDHSIAWGIAKTLAAHGAEMAFTYQGDALGKRVRPLADELGV
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCC
KHVLPCDVEDIASVDGVFTSLREAWGGLDFIVHAVAFSDKNELKGRYADTSRENFSRTML
CEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHH
ISCFSFTEIAKRAAELMPESGGSMITLTFDGSNRVMPNYNVMGVAKAALEASVRYLAADY
HHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GRRAIRVNAISAGPVRTLAGSGIGDARFMFAFQQKHSPLGRGITLEELGGSALYLLSDLS
CCEEEEEEEECCCCHHHHCCCCCCCHHHHEEEHHHCCCCCCCEEHHHCCCCEEEEEECCC
GGVTGEIHYVDSGYNVISMPRPEDLKAE
CCCEEEEEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]