Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 146300932

GI number: 146300932

Start: 3797579

End: 3798985

Strand: Direct

Name: phoA [H]

Synonym: Fjoh_3187

Alternate gene names: 146300932

Gene position: 3797579-3798985 (Clockwise)

Preceding gene: 146300931

Following gene: 146300933

Centisome position: 62.29

GC content: 36.39

Gene sequence:

>1407_bases
ATGAACAGACGCAGATTTCTTAAAGGCTCAACTTTATTAAGCGGATTGGCGGTTATGAGCCCAACGGTTATCTTTTCAAA
TGATTTGGAATCCATTTCAAAGAAAAATAAAAAAGCCAAAAATATTATATTTCTAATCAGTGACGGAATGAGCACTGGAA
CTCTGCAAATGGCGAATTTATATTCGCAGAATATTTTAAATAAAAATGGAAACTGGATGAATCTTTATGCAGAAAATAAA
GTTTCCAGAGCCTTAATGGATACAGCTTCTGCAAGCTCGGCAGTAACTGATTCTGCTGCGGCAAGTTCTTCTTTTGGAGG
CGGATATCGTGTTAGAAATGGTGTTTTAAATGTTGGACCAAATGGCGAAAAATACCTTCCAATCTGGCAGAAATTCAAAA
ATGCAGGGAAAAAAGCCGGCTGTGTTACCACAGTTACTATTACACATGCAACTCCGGCGGGATTCTGCGTGAACTCTGAC
AGCAGAAATGCTGAAAACGAAATTGCAGAAATGTATGCTGGTTTAGGATTAGACGTTCTGATGGGCGGCGGAGATGAATT
TTTTAATCCTGATAAAAGATCAGATAAAAAAGATATTTATAAAATCTATCAGGAAAAAGGGTATAAAGTAGTAAAAGATA
AAACTGAACTGGGAAATTTGAAAAAAGGCGCAAAAACTCTTGGAGTATTTTCTACAGGCGCATTGCCCTATTCTATTGAC
AGAACCAATATTCCGGAATTACAGCATACACCAACGCTTGCCGAAATGAGCAAAGCTGCTATTGACCAAATGAAAGATCA
CAAAGATGGTTTTGTTTTACTTATAGAAGGCGGAAAAGTAGACTGGGCAGCACATGCAAATGATATTGCAGCACTAATAC
ACGATCAGCTGGCATTTGATGAAGCTGTAAAAACGGCCATTGATTTTGCTGAAAAAGATACAGAAACGCTTGTTATTATA
ACTACTGACCACGGTAATGCCAATCCGGGGCTTATTTATGGCACAGATGCAACAAAAAAATTCAACAGCATTGCCGATTA
TAAATACACTAATGAATACATTTTGAATGCTATTCACAGTAATTTTAATTTACAGCAGATAAAAGACTGGATTTACCAGA
CCAACAAAATAAGCTTAAGCGATCAGGAAGCACAGCATTTACTTGATTTTTATTCTGGTTTGGAAAAAATGGAAGACGGA
TTGTACAATTACAAAAAACTACCTTTTAAAGCTTACTCAGAAATACAAAAAAAGCATAATAACATTGGATGGATCAGTAT
GGATCATTCTGGTGATTATGTAGAGTTAGCTGCTTTTGGTCCCGGAAGTGAACTTTTAAAACCTTTCGTAAAAAACACCG
ATATGCATTATTTAATGCTCGAAGCCGCAATGCAGGATAAATTATAA

Upstream 100 bases:

>100_bases
ATAAAATCATTCTAATAAAAAAATGACAATTAATTAACGCAACTATGTTTCATTAATTCCAAATTAAAAACTAAATTGCT
GAAAATTTAAATGAAGCAAA

Downstream 100 bases:

>100_bases
AATATTAATTTCGAGATATAAAAACAAACAGCCGGACAAGTTTTACGGCTGTTTTTTTGTTTATGAAAAACACAAAAGAT
CTTTTTTTGTTTAATAACCT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MNRRRFLKGSTLLSGLAVMSPTVIFSNDLESISKKNKKAKNIIFLISDGMSTGTLQMANLYSQNILNKNGNWMNLYAENK
VSRALMDTASASSAVTDSAAASSSFGGGYRVRNGVLNVGPNGEKYLPIWQKFKNAGKKAGCVTTVTITHATPAGFCVNSD
SRNAENEIAEMYAGLGLDVLMGGGDEFFNPDKRSDKKDIYKIYQEKGYKVVKDKTELGNLKKGAKTLGVFSTGALPYSID
RTNIPELQHTPTLAEMSKAAIDQMKDHKDGFVLLIEGGKVDWAAHANDIAALIHDQLAFDEAVKTAIDFAEKDTETLVII
TTDHGNANPGLIYGTDATKKFNSIADYKYTNEYILNAIHSNFNLQQIKDWIYQTNKISLSDQEAQHLLDFYSGLEKMEDG
LYNYKKLPFKAYSEIQKKHNNIGWISMDHSGDYVELAAFGPGSELLKPFVKNTDMHYLMLEAAMQDKL

Sequences:

>Translated_468_residues
MNRRRFLKGSTLLSGLAVMSPTVIFSNDLESISKKNKKAKNIIFLISDGMSTGTLQMANLYSQNILNKNGNWMNLYAENK
VSRALMDTASASSAVTDSAAASSSFGGGYRVRNGVLNVGPNGEKYLPIWQKFKNAGKKAGCVTTVTITHATPAGFCVNSD
SRNAENEIAEMYAGLGLDVLMGGGDEFFNPDKRSDKKDIYKIYQEKGYKVVKDKTELGNLKKGAKTLGVFSTGALPYSID
RTNIPELQHTPTLAEMSKAAIDQMKDHKDGFVLLIEGGKVDWAAHANDIAALIHDQLAFDEAVKTAIDFAEKDTETLVII
TTDHGNANPGLIYGTDATKKFNSIADYKYTNEYILNAIHSNFNLQQIKDWIYQTNKISLSDQEAQHLLDFYSGLEKMEDG
LYNYKKLPFKAYSEIQKKHNNIGWISMDHSGDYVELAAFGPGSELLKPFVKNTDMHYLMLEAAMQDKL
>Mature_468_residues
MNRRRFLKGSTLLSGLAVMSPTVIFSNDLESISKKNKKAKNIIFLISDGMSTGTLQMANLYSQNILNKNGNWMNLYAENK
VSRALMDTASASSAVTDSAAASSSFGGGYRVRNGVLNVGPNGEKYLPIWQKFKNAGKKAGCVTTVTITHATPAGFCVNSD
SRNAENEIAEMYAGLGLDVLMGGGDEFFNPDKRSDKKDIYKIYQEKGYKVVKDKTELGNLKKGAKTLGVFSTGALPYSID
RTNIPELQHTPTLAEMSKAAIDQMKDHKDGFVLLIEGGKVDWAAHANDIAALIHDQLAFDEAVKTAIDFAEKDTETLVII
TTDHGNANPGLIYGTDATKKFNSIADYKYTNEYILNAIHSNFNLQQIKDWIYQTNKISLSDQEAQHLLDFYSGLEKMEDG
LYNYKKLPFKAYSEIQKKHNNIGWISMDHSGDYVELAAFGPGSELLKPFVKNTDMHYLMLEAAMQDKL

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=335, Percent_Identity=25.9701492537313, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI294660770, Length=330, Percent_Identity=25.1515151515152, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI94721246, Length=335, Percent_Identity=25.0746268656716, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI157266296, Length=340, Percent_Identity=25, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI157266292, Length=334, Percent_Identity=25.1497005988024, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI294660772, Length=286, Percent_Identity=25.8741258741259, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI48994877, Length=410, Percent_Identity=27.5609756097561, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320689, Length=482, Percent_Identity=28.8381742738589, Blast_Score=152, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859923, Length=323, Percent_Identity=30.3405572755418, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19921912, Length=352, Percent_Identity=27.8409090909091, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355151, Length=335, Percent_Identity=27.7611940298507, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21355149, Length=347, Percent_Identity=26.5129682997118, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21358067, Length=478, Percent_Identity=25.1046025104602, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21355981, Length=340, Percent_Identity=28.2352941176471, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24651554, Length=479, Percent_Identity=24.6346555323591, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI85725200, Length=347, Percent_Identity=26.8011527377522, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI85815833, Length=347, Percent_Identity=26.8011527377522, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24657835, Length=364, Percent_Identity=25.2747252747253, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24657842, Length=506, Percent_Identity=23.7154150197628, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24657827, Length=356, Percent_Identity=26.123595505618, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24585213, Length=346, Percent_Identity=25.4335260115607, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24651556, Length=466, Percent_Identity=23.3905579399142, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51767; Mature: 51767

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRRFLKGSTLLSGLAVMSPTVIFSNDLESISKKNKKAKNIIFLISDGMSTGTLQMANL
CCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
YSQNILNKNGNWMNLYAENKVSRALMDTASASSAVTDSAAASSSFGGGYRVRNGVLNVGP
HHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCHHHCCHHHCCCCCCCCEEEECCEEEECC
NGEKYLPIWQKFKNAGKKAGCVTTVTITHATPAGFCVNSDSRNAENEIAEMYAGLGLDVL
CCCEECHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCEEEE
MGGGDEFFNPDKRSDKKDIYKIYQEKGYKVVKDKTELGNLKKGAKTLGVFSTGALPYSID
ECCCHHHCCCCCCCCHHHHHHHHHHCCCEEEECCHHHCCHHHCHHHHCEEECCCCCEECC
RTNIPELQHTPTLAEMSKAAIDQMKDHKDGFVLLIEGGKVDWAAHANDIAALIHDQLAFD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHCHHHHHHHHHHHHHHH
EAVKTAIDFAEKDTETLVIITTDHGNANPGLIYGTDATKKFNSIADYKYTNEYILNAIHS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHC
NFNLQQIKDWIYQTNKISLSDQEAQHLLDFYSGLEKMEDGLYNYKKLPFKAYSEIQKKHN
CCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NIGWISMDHSGDYVELAAFGPGSELLKPFVKNTDMHYLMLEAAMQDKL
CCCEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNRRRFLKGSTLLSGLAVMSPTVIFSNDLESISKKNKKAKNIIFLISDGMSTGTLQMANL
CCCCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHH
YSQNILNKNGNWMNLYAENKVSRALMDTASASSAVTDSAAASSSFGGGYRVRNGVLNVGP
HHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCHHHCCHHHCCCCCCCCEEEECCEEEECC
NGEKYLPIWQKFKNAGKKAGCVTTVTITHATPAGFCVNSDSRNAENEIAEMYAGLGLDVL
CCCEECHHHHHHHHCCCCCCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHCCCEEEE
MGGGDEFFNPDKRSDKKDIYKIYQEKGYKVVKDKTELGNLKKGAKTLGVFSTGALPYSID
ECCCHHHCCCCCCCCHHHHHHHHHHCCCEEEECCHHHCCHHHCHHHHCEEECCCCCEECC
RTNIPELQHTPTLAEMSKAAIDQMKDHKDGFVLLIEGGKVDWAAHANDIAALIHDQLAFD
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHCHHHHHHHHHHHHHHH
EAVKTAIDFAEKDTETLVIITTDHGNANPGLIYGTDATKKFNSIADYKYTNEYILNAIHS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHC
NFNLQQIKDWIYQTNKISLSDQEAQHLLDFYSGLEKMEDGLYNYKKLPFKAYSEIQKKHN
CCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NIGWISMDHSGDYVELAAFGPGSELLKPFVKNTDMHYLMLEAAMQDKL
CCCEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]