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The map label for this gene is map [H]

Identifier: 145300062

GI number: 145300062

Start: 3423320

End: 3424108

Strand: Direct

Name: map [H]

Synonym: ASA_3162

Alternate gene names: 145300062

Gene position: 3423320-3424108 (Clockwise)

Preceding gene: 145300040

Following gene: 145300063

Centisome position: 72.8

GC content: 57.29

Gene sequence:

>789_bases
ATGTCCATTAAAATCAAGACACCGGAAGAGATTGAAAAGATGCGCGTTGCCGGCCAGTTGGCCGCCGATGTGCTGATCAT
GATAGCCCCCCACGTCAAGGCAGGCGTCACCACAGATGAGCTGAACACCCTGTGCCACGACTATATTGTCGACGTGCAGC
AGGCTATTCCGGCTCCGCTCAACTATCACGGCTTCCCCAAGTCCATCTGCACCTCGGTGAACGACGTGATTTGCCATGGC
ATCCCCAACCACAAGAAATTGCGCGAAGGGGACATCATCAACCTGGACATCACAGTCATCAAGGATGGCTACCACGGCGA
TACCTCCGCCATGTTCATCGTGGGTGAGACCACACCGCTGCGTCGCCAGCTGTGCAAGGTCGCCCAGGAGAGCCTCTACG
CTGCCATCAAGCAGGTGCGCCCCGGCATGTGCATCACAGAGATAGGCGCCCTGATCCAGCCCATCGTCGAAAAGGCCGGT
TTCTCCGTGGTGCGTGACTACTGTGGTCACGGCATCGGCAGCGAATTCCACGAGGAGCCGCAAATCCTGCACTACCGCAA
TGGCGGCAAGCAGGAGCTCAAACCGGGCATGTGCTTCACCATAGAGCCCATGGTCAACAGCAAGAAATATCACTGCCGCA
TCAACCCGAAAGATGGCTGGACAGTGACCACCAAGGATGGCGGCGATTCCGCCCAGTGGGAACACACACTGCTGGTAACC
GACGATGGCTGCGAGGTGCTTACCCTGCGCCCCGATGACACTATCGACCGCTATATCAAACACGGTTAA

Upstream 100 bases:

>100_bases
TTGGGGATAAAATTTTGGCCTGATTGCAAACGCATCTATTTTTCATTACCTGATTGTTGGGCATCGACAGGATGCGCAGT
CAGGCCCCAAGAGAGATCCC

Downstream 100 bases:

>100_bases
CATCAAAAACCCCGGCGCCGAAGCGAGAAGGCACCGGGGTTTGTTTTTATCAGCCGCTGTGTATGCGTGCGACAAGAGCG
TGAGATGCCATGGATGCCAG

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSIKIKTPEEIEKMRVAGQLAADVLIMIAPHVKAGVTTDELNTLCHDYIVDVQQAIPAPLNYHGFPKSICTSVNDVICHG
IPNHKKLREGDIINLDITVIKDGYHGDTSAMFIVGETTPLRRQLCKVAQESLYAAIKQVRPGMCITEIGALIQPIVEKAG
FSVVRDYCGHGIGSEFHEEPQILHYRNGGKQELKPGMCFTIEPMVNSKKYHCRINPKDGWTVTTKDGGDSAQWEHTLLVT
DDGCEVLTLRPDDTIDRYIKHG

Sequences:

>Translated_262_residues
MSIKIKTPEEIEKMRVAGQLAADVLIMIAPHVKAGVTTDELNTLCHDYIVDVQQAIPAPLNYHGFPKSICTSVNDVICHG
IPNHKKLREGDIINLDITVIKDGYHGDTSAMFIVGETTPLRRQLCKVAQESLYAAIKQVRPGMCITEIGALIQPIVEKAG
FSVVRDYCGHGIGSEFHEEPQILHYRNGGKQELKPGMCFTIEPMVNSKKYHCRINPKDGWTVTTKDGGDSAQWEHTLLVT
DDGCEVLTLRPDDTIDRYIKHG
>Mature_261_residues
SIKIKTPEEIEKMRVAGQLAADVLIMIAPHVKAGVTTDELNTLCHDYIVDVQQAIPAPLNYHGFPKSICTSVNDVICHGI
PNHKKLREGDIINLDITVIKDGYHGDTSAMFIVGETTPLRRQLCKVAQESLYAAIKQVRPGMCITEIGALIQPIVEKAGF
SVVRDYCGHGIGSEFHEEPQILHYRNGGKQELKPGMCFTIEPMVNSKKYHCRINPKDGWTVTTKDGGDSAQWEHTLLVTD
DGCEVLTLRPDDTIDRYIKHG

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=260, Percent_Identity=47.3076923076923, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI40385867, Length=253, Percent_Identity=42.2924901185771, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI1786364, Length=263, Percent_Identity=62.7376425855513, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1788728, Length=261, Percent_Identity=26.0536398467433, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71996291, Length=250, Percent_Identity=47.6, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323273, Length=251, Percent_Identity=46.2151394422311, Blast_Score=218, Evalue=8e-58,
Organism=Drosophila melanogaster, GI21355531, Length=253, Percent_Identity=47.8260869565217, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24583427, Length=247, Percent_Identity=42.1052631578947, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29088; Mature: 28957

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKIKTPEEIEKMRVAGQLAADVLIMIAPHVKAGVTTDELNTLCHDYIVDVQQAIPAPL
CEEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NYHGFPKSICTSVNDVICHGIPNHKKLREGDIINLDITVIKDGYHGDTSAMFIVGETTPL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHH
RRQLCKVAQESLYAAIKQVRPGMCITEIGALIQPIVEKAGFSVVRDYCGHGIGSEFHEEP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCC
QILHYRNGGKQELKPGMCFTIEPMVNSKKYHCRINPKDGWTVTTKDGGDSAQWEHTLLVT
CEEEECCCCHHHCCCCCEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEE
DDGCEVLTLRPDDTIDRYIKHG
CCCCEEEEECCCHHHHHHHHCC
>Mature Secondary Structure 
SIKIKTPEEIEKMRVAGQLAADVLIMIAPHVKAGVTTDELNTLCHDYIVDVQQAIPAPL
EEECCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
NYHGFPKSICTSVNDVICHGIPNHKKLREGDIINLDITVIKDGYHGDTSAMFIVGETTPL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEEEEEEECCCHH
RRQLCKVAQESLYAAIKQVRPGMCITEIGALIQPIVEKAGFSVVRDYCGHGIGSEFHEEP
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCC
QILHYRNGGKQELKPGMCFTIEPMVNSKKYHCRINPKDGWTVTTKDGGDSAQWEHTLLVT
CEEEECCCCHHHCCCCCEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEE
DDGCEVLTLRPDDTIDRYIKHG
CCCCEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]