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The map label for this gene is apbE [H]

Identifier: 145299879

GI number: 145299879

Start: 3193590

End: 3194630

Strand: Direct

Name: apbE [H]

Synonym: ASA_2969

Alternate gene names: 145299879

Gene position: 3193590-3194630 (Clockwise)

Preceding gene: 145299876

Following gene: 145299880

Centisome position: 67.91

GC content: 59.75

Gene sequence:

>1041_bases
ATGTCGTTCACCTTTGTGTCGGCCCTGCGTGGCCTGCTGTTACCTATGGTGCTGATGTTGACGGCCTGCCAGCCAGCCCC
CACCCAGACCCGCATTCAGGGTAAAACCATGGGCACCTACTATGTGGTGACGGTGAACGATCCCTATCCGGGCGGTGAGG
CGGCGCTGCAGCGTGAACTTGATACCTTGCTGGCGCGGATGAACAAGGAGATCTCCACCTATGACAAGAGCTCCCTCATC
TCCCGCTTCAACCAGGGACCAGCCCGGGTGCCCATGATGATTCCGGCCACCATGGCAACCATAGTGCAACAGGGCATTGA
TACGGGACACCTCACTCGGGGCAAGCTGGATGTCACAGTGGGCCCTCTGGTCAACCTCTGGGGATTCGGGCCGGACATGC
GCCCGCTCAAGAAACCCAGTGACGCGCAGATCGCCAAGGCGCGTCAGATAGTGGGTATCGACAAGCTGACCCTGACCCCG
CAGGGAGACCACTTCCTGCTCAGCAAGGCAATCCCGGATCTCTACCTCGATCTCTCTACCCTGGGGGAAGGCGCCGCATC
GGATGAAATAGCCGTGTTGCTAGAGAGCAAGGGCGTCCACAACTACCTCATCGAAGTGGCAGGCGCCGTGCGCAGCAAAG
GCAACAACAGCAAGGGCAAGCCCTGGCGCGTCGCCATTGTCAAACCGGCGGATCAACCCGGCGCCTTCTCCGATGTTGTG
ACCCCCAATGGCATGGCCCTCAGCACCGCAGGCAGCTATCGCAACTACTACGAGCTCGATGGCCAGCGTTACTCCCACAT
TATCGACCCGGAGACAGGCGCCCCCGTGACCCACAAACTGGTCTCCGCCAGCGTGATTGCCAACACTGCGCTGGAGGCTG
ATGCCCTCGATACAGCGCTCATGGTGATGGGGCCCGATCAGGCAATGGCCTTCGCCAAAGAACACCAGCTGGCGGCCTAT
CTCATCATCAAGACGGAGCAAGGCTTTACCGCGAAATTCACTCCCCAGTTTGCCCCTTATCTGGTGCAAGAAAAGCCATA
A

Upstream 100 bases:

>100_bases
GTCCGCGATTTTGTGTGCCAGACGCTCGCCACCTGAAAGCCTCTTGGCTAAGCTGGCGACCCTATGGCACAACGCCCTGT
TTGAGATAGAGATATTGCTT

Downstream 100 bases:

>100_bases
GCCATATGGCCCCGCTGAAAAACAGCGGGGCCAGCCCATGTTCAATTACCTGTCTGCGCGCTTTGGTGGCACACTTCTGT
TATCCGCCCGTAGCGCCTTG

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSFTFVSALRGLLLPMVLMLTACQPAPTQTRIQGKTMGTYYVVTVNDPYPGGEAALQRELDTLLARMNKEISTYDKSSLI
SRFNQGPARVPMMIPATMATIVQQGIDTGHLTRGKLDVTVGPLVNLWGFGPDMRPLKKPSDAQIAKARQIVGIDKLTLTP
QGDHFLLSKAIPDLYLDLSTLGEGAASDEIAVLLESKGVHNYLIEVAGAVRSKGNNSKGKPWRVAIVKPADQPGAFSDVV
TPNGMALSTAGSYRNYYELDGQRYSHIIDPETGAPVTHKLVSASVIANTALEADALDTALMVMGPDQAMAFAKEHQLAAY
LIIKTEQGFTAKFTPQFAPYLVQEKP

Sequences:

>Translated_346_residues
MSFTFVSALRGLLLPMVLMLTACQPAPTQTRIQGKTMGTYYVVTVNDPYPGGEAALQRELDTLLARMNKEISTYDKSSLI
SRFNQGPARVPMMIPATMATIVQQGIDTGHLTRGKLDVTVGPLVNLWGFGPDMRPLKKPSDAQIAKARQIVGIDKLTLTP
QGDHFLLSKAIPDLYLDLSTLGEGAASDEIAVLLESKGVHNYLIEVAGAVRSKGNNSKGKPWRVAIVKPADQPGAFSDVV
TPNGMALSTAGSYRNYYELDGQRYSHIIDPETGAPVTHKLVSASVIANTALEADALDTALMVMGPDQAMAFAKEHQLAAY
LIIKTEQGFTAKFTPQFAPYLVQEKP
>Mature_345_residues
SFTFVSALRGLLLPMVLMLTACQPAPTQTRIQGKTMGTYYVVTVNDPYPGGEAALQRELDTLLARMNKEISTYDKSSLIS
RFNQGPARVPMMIPATMATIVQQGIDTGHLTRGKLDVTVGPLVNLWGFGPDMRPLKKPSDAQIAKARQIVGIDKLTLTPQ
GDHFLLSKAIPDLYLDLSTLGEGAASDEIAVLLESKGVHNYLIEVAGAVRSKGNNSKGKPWRVAIVKPADQPGAFSDVVT
PNGMALSTAGSYRNYYELDGQRYSHIIDPETGAPVTHKLVSASVIANTALEADALDTALMVMGPDQAMAFAKEHQLAAYL
IIKTEQGFTAKFTPQFAPYLVQEKP

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=348, Percent_Identity=45.6896551724138, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37377; Mature: 37245

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTFVSALRGLLLPMVLMLTACQPAPTQTRIQGKTMGTYYVVTVNDPYPGGEAALQREL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECEECCEEEEEEECCCCCCHHHHHHHHH
DTLLARMNKEISTYDKSSLISRFNQGPARVPMMIPATMATIVQQGIDTGHLTRGKLDVTV
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHCCCCCCEECCEEEEEC
GPLVNLWGFGPDMRPLKKPSDAQIAKARQIVGIDKLTLTPQGDHFLLSKAIPDLYLDLST
CCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEHHHCCCHHEEEHHH
LGEGAASDEIAVLLESKGVHNYLIEVAGAVRSKGNNSKGKPWRVAIVKPADQPGAFSDVV
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPNGMALSTAGSYRNYYELDGQRYSHIIDPETGAPVTHKLVSASVIANTALEADALDTAL
CCCCEEEECCCCCCCEEEECCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHCEEE
MVMGPDQAMAFAKEHQLAAYLIIKTEQGFTAKFTPQFAPYLVQEKP
EEECCHHHHHHHHHCCEEEEEEEEECCCCEEEECCCCCCEEECCCC
>Mature Secondary Structure 
SFTFVSALRGLLLPMVLMLTACQPAPTQTRIQGKTMGTYYVVTVNDPYPGGEAALQREL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECEECCEEEEEEECCCCCCHHHHHHHHH
DTLLARMNKEISTYDKSSLISRFNQGPARVPMMIPATMATIVQQGIDTGHLTRGKLDVTV
HHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHCCCCCCEECCEEEEEC
GPLVNLWGFGPDMRPLKKPSDAQIAKARQIVGIDKLTLTPQGDHFLLSKAIPDLYLDLST
CCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCEEEHHHCCCHHEEEHHH
LGEGAASDEIAVLLESKGVHNYLIEVAGAVRSKGNNSKGKPWRVAIVKPADQPGAFSDVV
HCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TPNGMALSTAGSYRNYYELDGQRYSHIIDPETGAPVTHKLVSASVIANTALEADALDTAL
CCCCEEEECCCCCCCEEEECCCEECEEECCCCCCCHHHHHHHHHHHHHHHHCHHHHCEEE
MVMGPDQAMAFAKEHQLAAYLIIKTEQGFTAKFTPQFAPYLVQEKP
EEECCHHHHHHHHHCCEEEEEEEEECCCCEEEECCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9473043; 11677609; 1904855 [H]