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The map label for this gene is glnB [H]

Identifier: 145296024

GI number: 145296024

Start: 2135597

End: 2135935

Strand: Reverse

Name: glnB [H]

Synonym: cgR_1947

Alternate gene names: 145296024

Gene position: 2135935-2135597 (Counterclockwise)

Preceding gene: 145296025

Following gene: 145296023

Centisome position: 64.45

GC content: 54.57

Gene sequence:

>339_bases
ATGAAACTCATCACCGCTATCGTCAAGCCGTTTACCCTCACTGACATTAAGGATGCACTCGAGCAGGCCGGTGTTCAGGG
AATGACAGTCACCGAAACCCAGGGATTTGGTCAGCAAAAGGGCCACACTGAGGTTTACCGCGGTGCAGAATACGCAGTTG
ATTTTGTTCCAAAGGTCAAGATCGAAGTCATCGTTGCTGACGCTCAGGCAGAAGAAGTTATCACCGCAATCGTCGATACC
GCCCGTACCGGCAAGGTAGGCGACGGCAAGGTGTGGATGACCACTGTTGACGAGCTCGTACGCGTGCGTACCGGTGAGCG
CGGCGAAGCTGCCCTCTAA

Upstream 100 bases:

>100_bases
CACGGAGAATCGGCTTATGACACCACAGGGCCGGAAATTCGTTAAGTTTGTTTAAAAATAGCTACATAACTCCGACCGTC
CTTTTGAAGGAGACGTCCCC

Downstream 100 bases:

>100_bases
GAAAGATTCGCGCTTACATCAATGAATGACCCAGCCCAGCTCCGCCATGGCGCCGAACAAGATGCCCTGGCGTTGCTGGG
CTCTTTGGCGCTTCCAGCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVKIEVIVADAQAEEVITAIVDT
ARTGKVGDGKVWMTTVDELVRVRTGERGEAAL

Sequences:

>Translated_112_residues
MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVKIEVIVADAQAEEVITAIVDT
ARTGKVGDGKVWMTTVDELVRVRTGERGEAAL
>Mature_112_residues
MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVKIEVIVADAQAEEVITAIVDT
ARTGKVGDGKVWMTTVDELVRVRTGERGEAAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=57.1428571428571, Blast_Score=144, Evalue=2e-36,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=56.25, Blast_Score=138, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12108; Mature: 12108

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVK
CCHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHCCCHHEEECCCCEE
IEVIVADAQAEEVITAIVDTARTGKVGDGKVWMTTVDELVRVRTGERGEAAL
EEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVK
CCHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHCCCHHEEECCCCEE
IEVIVADAQAEEVITAIVDTARTGKVGDGKVWMTTVDELVRVRTGERGEAAL
EEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]