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The map label for this gene is amt [H]

Identifier: 145296025

GI number: 145296025

Start: 2135991

End: 2137307

Strand: Reverse

Name: amt [H]

Synonym: cgR_1948

Alternate gene names: 145296025

Gene position: 2137307-2135991 (Counterclockwise)

Preceding gene: 145296026

Following gene: 145296024

Centisome position: 64.49

GC content: 55.96

Gene sequence:

>1317_bases
ATGAGCGCAGATCAAATCGCAGCAGTCTCTGGAAACTCAGCATGGATGCTCATGTCCGCATCGTTGGTGCTCTTGATGAC
ACCAGCACTGGCTTTCTTCTATGGCGGTATGTCCCGTCAAAAGTCCGTGCTCAACATGATGATGATGTCATTCGGAACCT
TGGGTGTCGTCAGCGTCATCTATATCCTGTGGGGATGGTCGATGTCCTATGGAACCCAGTCCATCGCAGGTATCTTTGCA
AACCCATTTGAGCTCTTCGGCCTTAAAGATTCCATCGTTGATGCAGAAGGCAACTACATCGAAGGCGCAGCCGGATACCC
CAACATTATCGATGTTGGATTCCAGCTCACCTTCGCAGTTATCTCGACTGCTCTTATCTCCGGTGCACTGGCAGAACGCG
TAAAGTTCTCCACCTGGCTCGTCTTCTCCGGAGCGTGGGCTACCTTCGCTTACTTCCCACTGGCACACATGGTCTGGGGT
GGCGGATTGCTCAGCCACTTCGAGCCCGGCTTCGCAGCCTGGATGTTCGGTGTCACCGACGGTGAAGCCAACATCGCACC
AATCGACTTCGCTGGCGGTACCGTCGTCCACATCTCCGCTGGTACGGCAGCATTGGTGCTGGCATTGATCGTCGGCAAGC
GTAAGACTTTTGGTAAGTCGATCGCTCGACCTCACAACCTCCCCTTCGTCATGCTCGGCGCAGCACTGCTGTGGTTCGGT
TGGTTTGGCTTCAACGGCGGTTCCGCATTCGCCGCCGACGGCATGGCTGGCTTGGCATGGGTAAACACCACCGCTGCTAC
CGCAGCAGCAATGCTCGGCTGGTTGGCCGTAGAAAAGATTCGCGACGGTAAGGCAACCTCCCTCGGTGCTGCCTCCGGTG
TTGTCGCCGGCCTCGTTGCCATCACCCCAGCTGCGGGATCTCTCACCCCAGTAACCTCCCTTATCCTCGGTGTCATCGGC
GGTGGCCTTGCGTGCCTCGGCGTTGGCCTGAAGTACCGCTTCGGTTTCGACGACTCCCTCGACGTAGTCGGTGTCCACCT
GGTCGCCGGTCTCTGGGGCACCATCGGTGTCGGCCTCCTCGCAACTGACGCAGGCTGGTTTACCGGTGGCGGATCCGATG
GATTTAAACTCTTTATTGTTCAGATTGTCATCGCACTTGTTGCCATGGTCTTCGCTGGAATTGTTACTGCAGTCATTGCA
TACATCCTCAAAGCAACCATGGGATGGCGCGTTGATGATGAAGTGGAACAGCAAGGCATTGATTTCAACGAACACGGAGA
ATCGGCTTATGACACCACAGGGCCGGAAATTCGTTAA

Upstream 100 bases:

>100_bases
AGTTAATTTCGGTTCGAGGGTCGTCAACCCCATCAGGCGCTCCTCGAATCCACAAAAATAGCTTGAATAAAACTTAAGGC
AAAAGGAGGTCGTCCACGCT

Downstream 100 bases:

>100_bases
GTTTGTTTAAAAATAGCTACATAACTCCGACCGTCCTTTTGAAGGAGACGTCCCCATGAAACTCATCACCGCTATCGTCA
AGCCGTTTACCCTCACTGAC

Product: hypothetical protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSADQIAAVSGNSAWMLMSASLVLLMTPALAFFYGGMSRQKSVLNMMMMSFGTLGVVSVIYILWGWSMSYGTQSIAGIFA
NPFELFGLKDSIVDAEGNYIEGAAGYPNIIDVGFQLTFAVISTALISGALAERVKFSTWLVFSGAWATFAYFPLAHMVWG
GGLLSHFEPGFAAWMFGVTDGEANIAPIDFAGGTVVHISAGTAALVLALIVGKRKTFGKSIARPHNLPFVMLGAALLWFG
WFGFNGGSAFAADGMAGLAWVNTTAATAAAMLGWLAVEKIRDGKATSLGAASGVVAGLVAITPAAGSLTPVTSLILGVIG
GGLACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTIGVGLLATDAGWFTGGGSDGFKLFIVQIVIALVAMVFAGIVTAVIA
YILKATMGWRVDDEVEQQGIDFNEHGESAYDTTGPEIR

Sequences:

>Translated_438_residues
MSADQIAAVSGNSAWMLMSASLVLLMTPALAFFYGGMSRQKSVLNMMMMSFGTLGVVSVIYILWGWSMSYGTQSIAGIFA
NPFELFGLKDSIVDAEGNYIEGAAGYPNIIDVGFQLTFAVISTALISGALAERVKFSTWLVFSGAWATFAYFPLAHMVWG
GGLLSHFEPGFAAWMFGVTDGEANIAPIDFAGGTVVHISAGTAALVLALIVGKRKTFGKSIARPHNLPFVMLGAALLWFG
WFGFNGGSAFAADGMAGLAWVNTTAATAAAMLGWLAVEKIRDGKATSLGAASGVVAGLVAITPAAGSLTPVTSLILGVIG
GGLACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTIGVGLLATDAGWFTGGGSDGFKLFIVQIVIALVAMVFAGIVTAVIA
YILKATMGWRVDDEVEQQGIDFNEHGESAYDTTGPEIR
>Mature_437_residues
SADQIAAVSGNSAWMLMSASLVLLMTPALAFFYGGMSRQKSVLNMMMMSFGTLGVVSVIYILWGWSMSYGTQSIAGIFAN
PFELFGLKDSIVDAEGNYIEGAAGYPNIIDVGFQLTFAVISTALISGALAERVKFSTWLVFSGAWATFAYFPLAHMVWGG
GLLSHFEPGFAAWMFGVTDGEANIAPIDFAGGTVVHISAGTAALVLALIVGKRKTFGKSIARPHNLPFVMLGAALLWFGW
FGFNGGSAFAADGMAGLAWVNTTAATAAAMLGWLAVEKIRDGKATSLGAASGVVAGLVAITPAAGSLTPVTSLILGVIGG
GLACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTIGVGLLATDAGWFTGGGSDGFKLFIVQIVIALVAMVFAGIVTAVIAY
ILKATMGWRVDDEVEQQGIDFNEHGESAYDTTGPEIR

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=436, Percent_Identity=41.2844036697248, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17550182, Length=452, Percent_Identity=30.3097345132743, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550180, Length=449, Percent_Identity=30.0668151447661, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=447, Percent_Identity=25.0559284116331, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=24.1071428571429, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6325396, Length=443, Percent_Identity=30.6997742663657, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321559, Length=439, Percent_Identity=29.6127562642369, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324187, Length=439, Percent_Identity=29.3849658314351, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI161078314, Length=438, Percent_Identity=28.310502283105, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45585; Mature: 45454

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADQIAAVSGNSAWMLMSASLVLLMTPALAFFYGGMSRQKSVLNMMMMSFGTLGVVSVI
CCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHH
YILWGWSMSYGTQSIAGIFANPFELFGLKDSIVDAEGNYIEGAAGYPNIIDVGFQLTFAV
HHHHCCCHHCCCHHHHHHHCCCHHHHCCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHH
ISTALISGALAERVKFSTWLVFSGAWATFAYFPLAHMVWGGGLLSHFEPGFAAWMFGVTD
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEEEEEC
GEANIAPIDFAGGTVVHISAGTAALVLALIVGKRKTFGKSIARPHNLPFVMLGAALLWFG
CCCCEEEEEECCCEEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
WFGFNGGSAFAADGMAGLAWVNTTAATAAAMLGWLAVEKIRDGKATSLGAASGVVAGLVA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ITPAAGSLTPVTSLILGVIGGGLACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTIGVGLL
HCCCCCCCHHHHHHHHHHHCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHE
ATDAGWFTGGGSDGFKLFIVQIVIALVAMVFAGIVTAVIAYILKATMGWRVDDEVEQQGI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
DFNEHGESAYDTTGPEIR
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SADQIAAVSGNSAWMLMSASLVLLMTPALAFFYGGMSRQKSVLNMMMMSFGTLGVVSVI
CCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHH
YILWGWSMSYGTQSIAGIFANPFELFGLKDSIVDAEGNYIEGAAGYPNIIDVGFQLTFAV
HHHHCCCHHCCCHHHHHHHCCCHHHHCCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHH
ISTALISGALAERVKFSTWLVFSGAWATFAYFPLAHMVWGGGLLSHFEPGFAAWMFGVTD
HHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCHHHHCCCCHHEEEEEEEC
GEANIAPIDFAGGTVVHISAGTAALVLALIVGKRKTFGKSIARPHNLPFVMLGAALLWFG
CCCCEEEEEECCCEEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
WFGFNGGSAFAADGMAGLAWVNTTAATAAAMLGWLAVEKIRDGKATSLGAASGVVAGLVA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ITPAAGSLTPVTSLILGVIGGGLACLGVGLKYRFGFDDSLDVVGVHLVAGLWGTIGVGLL
HCCCCCCCHHHHHHHHHHHCCHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHE
ATDAGWFTGGGSDGFKLFIVQIVIALVAMVFAGIVTAVIAYILKATMGWRVDDEVEQQGI
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCC
DFNEHGESAYDTTGPEIR
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]