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The map label for this gene is pcaF [H]

Identifier: 145294705

GI number: 145294705

Start: 710443

End: 711648

Strand: Direct

Name: pcaF [H]

Synonym: cgR_0653

Alternate gene names: 145294705

Gene position: 710443-711648 (Clockwise)

Preceding gene: 145294704

Following gene: 145294706

Centisome position: 21.44

GC content: 55.14

Gene sequence:

>1206_bases
GTGAGCGATCGAACCGCATATCTTGTCTCCGGCAAAAGAACACCAGTGGGCCGCTACGGTGGGGCACTCTCCTCAGTTCG
CCCTGATGATCTAGCAGCCCTCACCATCACTGCCGTCATTGAAGAATCAGGCATTGATCCATCAGTGGTGGATGAGGTCA
TTTTAGGAAATGCCAACGGTGCTGGCGAAGAAAACCGCAACGTTGCACGCCTGGCATGGCTTTTGGCGGGATACCCAGAC
ACCGTTCCTGGAATTACCGTGAACAGGCTTTGCGCTTCTGGAATGTCTGCCATTGCGTTGGCTACTGCGATGGTGGAATC
AGGACAAGCTGATGTGGTGGTTGCCGGTGGTGTGGAATCTATGTCGAGGGCACCGTGGGTGCAGGAAAAGCCACGATCGG
CTTTCGCTAAGCCGGGTGAGATTTTTGATACCTCGATTGGTTGGCGTTTCGTGAACCCTATTTTCCAGGAGCAGGAGAAG
ACCACGTTCTCCATGCCGGAAACTGCGGAAGAAGTCGCTCGGGTTAAGGGCGTGTCACGTGAGGAAGCTGATGTGTTTGC
AGCGACCTCGCAAGCTCGTGCTTTGGCTGCGATTTCGGCTGGACATTTTGATTCTGAAATCGTCCCGGTTGAAATCACTG
ATCGTAAAGGCAACGTGACCGTGGTGTCGCAAGACGAAGGTCCACGTGAAGGCACCACTACTCAGGTGTTGGCAAAGCTG
CGGCCGGTGGTGCGTGGGGGAGAGGTAGTTACTGCCGGAAATTCCTCGTCGCTTAATGATGGAGCGTCCGCCATCATCGT
GGCTAGCGCTGAAGCTGTAGAGAAATACGGTTTGACGGCTCGTGCGAAGGTTGTTGGTGCCAGCAATGCAGGTCTTGCCC
CAGAGATCATGGGTATGGGGCCTGTGCCAGCTACTAAGAAACTTCTCAAGCGCACTGGCTGGAGCGTCGAAGACCTGGAC
GCTGTAGAGCTCAACGAAGCATTCGCAACTCAGTCGCTTGCCTGCATGCGGGAACTCGAACTTGATCCAGAAAAGGTCAA
TGCGTGGGGCGGTGCCATTGCGTTGGGCCACCCACTGGGTTCTTCTGGTTCCAGAATCACCATCACACTGCTTAACCGCC
TGGAAAAAGAAGGCGGATCCAAGGGCATTGCCACCATGTGTATCGGAGTCGGTCAGGGCTCCGCGATCGCTATCGAAAGG
GTGTAG

Upstream 100 bases:

>100_bases
CGCCAGTTTTCTTCGGACGCTAAAAGACGTTAAAGATGCTAAGAAATCCTCTAAGAAACCCAGTCACACGCACATCTATA
TATAAGGAGTAGTTGAAAAC

Downstream 100 bases:

>100_bases
TTAATCATGAGCTTTGATTTTGATGCTTTGACCATCACGGAATCTGACGACCTTTTGATCATCGAGCTTTCTCGGCCAGA
GGTCCGTAACGCCATTGACC

Product: hypothetical protein

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSDRTAYLVSGKRTPVGRYGGALSSVRPDDLAALTITAVIEESGIDPSVVDEVILGNANGAGEENRNVARLAWLLAGYPD
TVPGITVNRLCASGMSAIALATAMVESGQADVVVAGGVESMSRAPWVQEKPRSAFAKPGEIFDTSIGWRFVNPIFQEQEK
TTFSMPETAEEVARVKGVSREEADVFAATSQARALAAISAGHFDSEIVPVEITDRKGNVTVVSQDEGPREGTTTQVLAKL
RPVVRGGEVVTAGNSSSLNDGASAIIVASAEAVEKYGLTARAKVVGASNAGLAPEIMGMGPVPATKKLLKRTGWSVEDLD
AVELNEAFATQSLACMRELELDPEKVNAWGGAIALGHPLGSSGSRITITLLNRLEKEGGSKGIATMCIGVGQGSAIAIER
V

Sequences:

>Translated_401_residues
MSDRTAYLVSGKRTPVGRYGGALSSVRPDDLAALTITAVIEESGIDPSVVDEVILGNANGAGEENRNVARLAWLLAGYPD
TVPGITVNRLCASGMSAIALATAMVESGQADVVVAGGVESMSRAPWVQEKPRSAFAKPGEIFDTSIGWRFVNPIFQEQEK
TTFSMPETAEEVARVKGVSREEADVFAATSQARALAAISAGHFDSEIVPVEITDRKGNVTVVSQDEGPREGTTTQVLAKL
RPVVRGGEVVTAGNSSSLNDGASAIIVASAEAVEKYGLTARAKVVGASNAGLAPEIMGMGPVPATKKLLKRTGWSVEDLD
AVELNEAFATQSLACMRELELDPEKVNAWGGAIALGHPLGSSGSRITITLLNRLEKEGGSKGIATMCIGVGQGSAIAIER
V
>Mature_400_residues
SDRTAYLVSGKRTPVGRYGGALSSVRPDDLAALTITAVIEESGIDPSVVDEVILGNANGAGEENRNVARLAWLLAGYPDT
VPGITVNRLCASGMSAIALATAMVESGQADVVVAGGVESMSRAPWVQEKPRSAFAKPGEIFDTSIGWRFVNPIFQEQEKT
TFSMPETAEEVARVKGVSREEADVFAATSQARALAAISAGHFDSEIVPVEITDRKGNVTVVSQDEGPREGTTTQVLAKLR
PVVRGGEVVTAGNSSSLNDGASAIIVASAEAVEKYGLTARAKVVGASNAGLAPEIMGMGPVPATKKLLKRTGWSVEDLDA
VELNEAFATQSLACMRELELDPEKVNAWGGAIALGHPLGSSGSRITITLLNRLEKEGGSKGIATMCIGVGQGSAIAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=396, Percent_Identity=41.6666666666667, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI148539872, Length=399, Percent_Identity=42.6065162907268, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI4501853, Length=403, Percent_Identity=40.6947890818859, Blast_Score=255, Evalue=5e-68,
Organism=Homo sapiens, GI4557237, Length=398, Percent_Identity=35.929648241206, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=32.7868852459016, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI194353979, Length=396, Percent_Identity=31.5656565656566, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=51.8703241895262, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=43.75, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI87082165, Length=397, Percent_Identity=41.5617128463476, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=42.2885572139304, Blast_Score=274, Evalue=8e-75,
Organism=Escherichia coli, GI1788683, Length=416, Percent_Identity=28.8461538461538, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI133906874, Length=405, Percent_Identity=38.2716049382716, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI25147385, Length=406, Percent_Identity=33.9901477832512, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=35.9605911330049, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=29.3838862559242, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=411, Percent_Identity=30.1703163017032, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325229, Length=405, Percent_Identity=37.037037037037, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322031, Length=406, Percent_Identity=33.743842364532, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=44.8877805486284, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=39.25, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24640423, Length=399, Percent_Identity=37.844611528822, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32.7058823529412, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41746; Mature: 41614

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRTAYLVSGKRTPVGRYGGALSSVRPDDLAALTITAVIEESGIDPSVVDEVILGNANG
CCCCEEEEEECCCCCCHHCCCCCCCCCCCCHHEEEEEEEHHHCCCCHHHHHHHHCCCCCC
AGEENRNVARLAWLLAGYPDTVPGITVNRLCASGMSAIALATAMVESGQADVVVAGGVES
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCHHH
MSRAPWVQEKPRSAFAKPGEIFDTSIGWRFVNPIFQEQEKTTFSMPETAEEVARVKGVSR
HHCCCCCCCCCHHHCCCCCCEEECCCCHHHHCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
EEADVFAATSQARALAAISAGHFDSEIVPVEITDRKGNVTVVSQDEGPREGTTTQVLAKL
HHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHH
RPVVRGGEVVTAGNSSSLNDGASAIIVASAEAVEKYGLTARAKVVGASNAGLAPEIMGMG
HHHHCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
PVPATKKLLKRTGWSVEDLDAVELNEAFATQSLACMRELELDPEKVNAWGGAIALGHPLG
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCC
SSGSRITITLLNRLEKEGGSKGIATMCIGVGQGSAIAIERV
CCCCEEEEHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEC
>Mature Secondary Structure 
SDRTAYLVSGKRTPVGRYGGALSSVRPDDLAALTITAVIEESGIDPSVVDEVILGNANG
CCCEEEEEECCCCCCHHCCCCCCCCCCCCHHEEEEEEEHHHCCCCHHHHHHHHCCCCCC
AGEENRNVARLAWLLAGYPDTVPGITVNRLCASGMSAIALATAMVESGQADVVVAGGVES
CCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCHHH
MSRAPWVQEKPRSAFAKPGEIFDTSIGWRFVNPIFQEQEKTTFSMPETAEEVARVKGVSR
HHCCCCCCCCCHHHCCCCCCEEECCCCHHHHCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
EEADVFAATSQARALAAISAGHFDSEIVPVEITDRKGNVTVVSQDEGPREGTTTQVLAKL
HHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHH
RPVVRGGEVVTAGNSSSLNDGASAIIVASAEAVEKYGLTARAKVVGASNAGLAPEIMGMG
HHHHCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCEEEEEEECCCCCCCCHHCCCC
PVPATKKLLKRTGWSVEDLDAVELNEAFATQSLACMRELELDPEKVNAWGGAIALGHPLG
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCC
SSGSRITITLLNRLEKEGGSKGIATMCIGVGQGSAIAIERV
CCCCEEEEHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]