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The map label for this gene is paaF [C]

Identifier: 145294704

GI number: 145294704

Start: 709580

End: 710365

Strand: Direct

Name: paaF [C]

Synonym: cgR_0652

Alternate gene names: 145294704

Gene position: 709580-710365 (Clockwise)

Preceding gene: 145294703

Following gene: 145294705

Centisome position: 21.41

GC content: 50.51

Gene sequence:

>786_bases
GTGATCGATCTTAATATCCACCGCATTGATGAGCACGCAAGTTATGCCGAAGTAGTGCTCAACGATCCATCAACTCTCAA
CGCCATCGCAGAACAAGATCTTCTTGATATTTCTGCCGCTTACACTGAAGCGGAAAAGGCTGGGGTTCGAGCACTTTTAC
TCCGCGGCGAAGGCAAAGGATTCTGCTCGGGACGAAACATCAAAGGTGTCGTGCCAGCCGAAGATGATGCAGCAGATTAC
TTAGCTAACAAAGTCACTCCAGTTCTCAAGCAGATGAGTGCTTTCCCAGCTCCAACGTTTGCTGCAGTCCACGGCGCGTG
CTTGGGCGTTGGGCTTGGGCTGGCCATTGCCTCTGACATTGTTTATGTTGCTGAAAAATCTAAGTTTGGCTCACCTTTTG
CCAACTTAGGCGCAACCCTTGATTCCGGCGGACACGCACTTTTCGTGGAACGCCTTGGTGCCCACCGCACCATGGATCTC
ATCGTCACCGGTGAACTGATCTCCGGAGCAGAAGCTGTCAACAGTGGTTTGTTCTCCAGACTGGTTCCCACTGAAGAGCT
TCTAGACTTCACCCGAGACAAGGTGATCCGTGCAGCTCAAGGTGCAACTCAAGCATTTGTTACCAGTAAAGCCTTGGTTC
AAGATATCCGCGACAACTGCACTGGATTATGGGATTCCGTAGCCGTGGAAAACGAAGAACAAGGTCGACTGTGCTCTTCA
ACTGATTACGCAGAAGGATTTGCTGCTTTCAATGAAAAGCGCACGCCAGTTTTCTTCGGACGCTAA

Upstream 100 bases:

>100_bases
CGAGCCTGATGAAGTTGCAGCTGGTTACGTGCTGACCTGCCAAACCCGACCAACTGGTGATTGCATCACTGTTGACTACG
ACGCGTAAAGGACAAGGTCA

Downstream 100 bases:

>100_bases
AAGACGTTAAAGATGCTAAGAAATCCTCTAAGAAACCCAGTCACACGCACATCTATATATAAGGAGTAGTTGAAAACGTG
AGCGATCGAACCGCATATCT

Product: hypothetical protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MIDLNIHRIDEHASYAEVVLNDPSTLNAIAEQDLLDISAAYTEAEKAGVRALLLRGEGKGFCSGRNIKGVVPAEDDAADY
LANKVTPVLKQMSAFPAPTFAAVHGACLGVGLGLAIASDIVYVAEKSKFGSPFANLGATLDSGGHALFVERLGAHRTMDL
IVTGELISGAEAVNSGLFSRLVPTEELLDFTRDKVIRAAQGATQAFVTSKALVQDIRDNCTGLWDSVAVENEEQGRLCSS
TDYAEGFAAFNEKRTPVFFGR

Sequences:

>Translated_261_residues
MIDLNIHRIDEHASYAEVVLNDPSTLNAIAEQDLLDISAAYTEAEKAGVRALLLRGEGKGFCSGRNIKGVVPAEDDAADY
LANKVTPVLKQMSAFPAPTFAAVHGACLGVGLGLAIASDIVYVAEKSKFGSPFANLGATLDSGGHALFVERLGAHRTMDL
IVTGELISGAEAVNSGLFSRLVPTEELLDFTRDKVIRAAQGATQAFVTSKALVQDIRDNCTGLWDSVAVENEEQGRLCSS
TDYAEGFAAFNEKRTPVFFGR
>Mature_261_residues
MIDLNIHRIDEHASYAEVVLNDPSTLNAIAEQDLLDISAAYTEAEKAGVRALLLRGEGKGFCSGRNIKGVVPAEDDAADY
LANKVTPVLKQMSAFPAPTFAAVHGACLGVGLGLAIASDIVYVAEKSKFGSPFANLGATLDSGGHALFVERLGAHRTMDL
IVTGELISGAEAVNSGLFSRLVPTEELLDFTRDKVIRAAQGATQAFVTSKALVQDIRDNCTGLWDSVAVENEEQGRLCSS
TDYAEGFAAFNEKRTPVFFGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=247, Percent_Identity=28.7449392712551, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=27.4900398406374, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI70995211, Length=185, Percent_Identity=28.6486486486486, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787659, Length=248, Percent_Identity=27.4193548387097, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787660, Length=251, Percent_Identity=31.4741035856574, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=238, Percent_Identity=26.0504201680672, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=27.7153558052434, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=27.6264591439689, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=265, Percent_Identity=27.5471698113208, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=182, Percent_Identity=27.4725274725275, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=30, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=30, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=27.8625954198473, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24653139, Length=267, Percent_Identity=28.0898876404494, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=188, Percent_Identity=30.8510638297872, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27767; Mature: 27767

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLNIHRIDEHASYAEVVLNDPSTLNAIAEQDLLDISAAYTEAEKAGVRALLLRGEGKG
CCCCEEEECCCCCCEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FCSGRNIKGVVPAEDDAADYLANKVTPVLKQMSAFPAPTFAAVHGACLGVGLGLAIASDI
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYVAEKSKFGSPFANLGATLDSGGHALFVERLGAHRTMDLIVTGELISGAEAVNSGLFSR
HHEECCCCCCCCHHHHCCEECCCCCEEHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHH
LVPTEELLDFTRDKVIRAAQGATQAFVTSKALVQDIRDNCTGLWDSVAVENEEQGRLCSS
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC
TDYAEGFAAFNEKRTPVFFGR
CCHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MIDLNIHRIDEHASYAEVVLNDPSTLNAIAEQDLLDISAAYTEAEKAGVRALLLRGEGKG
CCCCEEEECCCCCCEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FCSGRNIKGVVPAEDDAADYLANKVTPVLKQMSAFPAPTFAAVHGACLGVGLGLAIASDI
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYVAEKSKFGSPFANLGATLDSGGHALFVERLGAHRTMDLIVTGELISGAEAVNSGLFSR
HHEECCCCCCCCHHHHCCEECCCCCEEHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHH
LVPTEELLDFTRDKVIRAAQGATQAFVTSKALVQDIRDNCTGLWDSVAVENEEQGRLCSS
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC
TDYAEGFAAFNEKRTPVFFGR
CCHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA