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The map label for this gene is gluQ [H]

Identifier: 145294355

GI number: 145294355

Start: 337462

End: 338343

Strand: Direct

Name: gluQ [H]

Synonym: cgR_0310

Alternate gene names: 145294355

Gene position: 337462-338343 (Clockwise)

Preceding gene: 145294354

Following gene: 145294357

Centisome position: 10.18

GC content: 60.88

Gene sequence:

>882_bases
ATGGCAGGACGATACGCACCATCACCAAGCGGTGACCTTCACTTCGGCAACCTCCGCACAGCGCTTCTGGCCTGGCTGTT
CGCGCGCTCCGAAGGCAAACAGTTCCTCATGCGGGTCGAAGACATCGACGAACAACGCTCATCGAAGGAATCCGCCGAAA
GCCAACTCGCAGACCTGTCCGCCCTGGGTCTCGACTGGGATGGCGACGTCCTCTACCAATCCACACGCTACGACGCCTAC
CGCGCAGCCCTTGAAAAACTAGACACCTACGAATGTTATTGCTCGCGCCGGGACATCCAAGAAGCCTCGCGGGCACCCCA
TGCGGCTCCGGGAGTGTATCCGGGAACGTGTAGGGAATTGAAGGAGGAGGAACGCGTCGAAAAGCGTGCAAGCTTGGCCG
CGCAAAACCGGCACCCCGCCATCCGCCTGCGCGCGCAGGTAACTTCTTTTGATTTTTATGACCGACTTCGCGGCCCCCAA
ACCGGCCCCGTGGATGATTTCATTTTGCTGCGCGGCGGCCAGGAACCCGGATGGGCATACAACTTAGCCGTCGTCGTCGA
CGATGCCTACCAAGGCGTTGACCAGGTAGTTCGCGGCGACGACCTGCTTGATTCCGCCGCCCGCCAAGCCTACCTCGGCT
CGCTGCTGGGCACTCCCGCCCCCGAATACATCCACGTGCCGCTCGTGCTCAACGCCCACGGCCAGCGCCTCGCCAAACGC
GACGGGGCAGTGACGCTTAAAGAAATGCTTATCGACGCCACCCTCCCCACCATCTTCTCCCGGCTCGCCCACTCACTCGG
CTACGAGGAAGTGGACTCTGCACCCCAACTGCTAGAGGTCTTCGACCCCACAAACTTAAGCCGGGAACCGTTTATTTACT
GA

Upstream 100 bases:

>100_bases
CACCCCTGAAAACCTCCTCAACTATCCCGGAGTGATCATCTCCACCGTTCAGGAGAACCCATCCGAAACATGGCGACAAG
TGAACATCTAATCTAGAAAC

Downstream 100 bases:

>100_bases
GGCTCAGAGGGAGGGGTCATTCCATCTGGAGGGGTCATACCCTCTCCTGGGCCACCCATTCCACCGCCACTTGGGCCTCC
ACCCATTCCGCCACCACTTG

Product: glutamyl-Q tRNA(Asp) synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKQFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAY
RAALEKLDTYECYCSRRDIQEASRAPHAAPGVYPGTCRELKEEERVEKRASLAAQNRHPAIRLRAQVTSFDFYDRLRGPQ
TGPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
DGAVTLKEMLIDATLPTIFSRLAHSLGYEEVDSAPQLLEVFDPTNLSREPFIY

Sequences:

>Translated_293_residues
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKQFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAY
RAALEKLDTYECYCSRRDIQEASRAPHAAPGVYPGTCRELKEEERVEKRASLAAQNRHPAIRLRAQVTSFDFYDRLRGPQ
TGPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
DGAVTLKEMLIDATLPTIFSRLAHSLGYEEVDSAPQLLEVFDPTNLSREPFIY
>Mature_292_residues
AGRYAPSPSGDLHFGNLRTALLAWLFARSEGKQFLMRVEDIDEQRSSKESAESQLADLSALGLDWDGDVLYQSTRYDAYR
AALEKLDTYECYCSRRDIQEASRAPHAAPGVYPGTCRELKEEERVEKRASLAAQNRHPAIRLRAQVTSFDFYDRLRGPQT
GPVDDFILLRGGQEPGWAYNLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKRD
GAVTLKEMLIDATLPTIFSRLAHSLGYEEVDSAPQLLEVFDPTNLSREPFIY

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=314, Percent_Identity=31.5286624203822, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI226510925, Length=254, Percent_Identity=42.5196850393701, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1788743, Length=302, Percent_Identity=33.7748344370861, Blast_Score=167, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI71993394, Length=254, Percent_Identity=28.740157480315, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324540, Length=312, Percent_Identity=29.8076923076923, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24665321, Length=266, Percent_Identity=32.3308270676692, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 32694; Mature: 32563

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRYAPSPSGDLHFGNLRTALLAWLFARSEGKQFLMRVEDIDEQRSSKESAESQLADLS
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLDWDGDVLYQSTRYDAYRAALEKLDTYECYCSRRDIQEASRAPHAAPGVYPGTCREL
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
KEEERVEKRASLAAQNRHPAIRLRAQVTSFDFYDRLRGPQTGPVDDFILLRGGQEPGWAY
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEE
NLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHC
DGAVTLKEMLIDATLPTIFSRLAHSLGYEEVDSAPQLLEVFDPTNLSREPFIY
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGRYAPSPSGDLHFGNLRTALLAWLFARSEGKQFLMRVEDIDEQRSSKESAESQLADLS
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGLDWDGDVLYQSTRYDAYRAALEKLDTYECYCSRRDIQEASRAPHAAPGVYPGTCREL
HHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
KEEERVEKRASLAAQNRHPAIRLRAQVTSFDFYDRLRGPQTGPVDDFILLRGGQEPGWAY
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEE
NLAVVVDDAYQGVDQVVRGDDLLDSAARQAYLGSLLGTPAPEYIHVPLVLNAHGQRLAKR
EEEEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHC
DGAVTLKEMLIDATLPTIFSRLAHSLGYEEVDSAPQLLEVFDPTNLSREPFIY
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]