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The map label for this gene is hisZ [H]

Identifier: 138896664

GI number: 138896664

Start: 3122508

End: 3123692

Strand: Reverse

Name: hisZ [H]

Synonym: GTNG_3027

Alternate gene names: 138896664

Gene position: 3123692-3122508 (Counterclockwise)

Preceding gene: 138896665

Following gene: 138896663

Centisome position: 87.98

GC content: 55.44

Gene sequence:

>1185_bases
ATGGCAAAAAGGCTGTTCATGTTTGAAAAACCGTTAGGCATGCGCGATACGCTGCCGTTTTTATATGAAATGAAAAAGCA
AGTGCGCCGCACGATGGCGGAGGAAATCGAACGGTGGGGCTATGAGTTTATCGAGACGCCGACGTTGGAATATTATGAAA
CGGTTGGGACGGCGTCGGCGATTGCTGATCATCGGTTGTTTAAGCTGTTGGATCAGCAAGGGCATACGCTCGTGTTGCGG
CCGGATATGACGGCGCCGATCGCTCGGGTGGCGGCATCGCGGCTGTATGAGGACAGCAACCCGCTTCGATTGGCGTACAA
TGCCAACGTGTTCCGCGCTCAGCAGCGTGAAGGTGGCCGGCCGGCGGAGTTTGAACAAATCGGTGTCGAGTTGATCGGCG
ATGGCACGGTGATGGCTGATGCCGAGGTGATTAGCTTAATGGCCGCGTTGTTGAAGCGCGTGGGGCTTAGTCACTTTTCG
GTGGCGGTCGGCCATATCGGCTATGTGAACGCGTTGTTTTTGGAGATTTTAGGGAATGAAGAGCGGGCGAGCGTGCTGCG
CCGTTTCTTATATGAAAAAAATTACGTTGGCTACCGCGAACATGTGAAGTCGTTGCCGCTTTCGTCCATTGATCAAAAAC
GTCTTCTCGATCTTCTTTCCTTGCGCGGCGGCAGTGAAGCGATCGAGGCGGCAAAAGCGCTGGTGACAAGCGAGGAAGGA
CAGCGAGCAGCGGATGAGCTGGCGGCGCTGTTGGCAGCGCTTAAGACGTATGGGGTGTCCGAGGCGGTGAAACTCGATAT
GGCACTCGTGAGCCATATGAGCTATTATACTGGCATTTTGTTTGAAGTATACGCCGAGCAAGTCGGGTTTCCGATTGGCA
ACGGCGGTCGGTATGATGATTTATTGGCCAAATTCTCCCGTCCGGCGCCGGCGACGGGGTTTGGGTTGCGTGTTGATCGG
CTGATCGAGGCGATTGGCGAGACGGACGTCCGGGGAGAAATTGAGTGCATCGTCTTCAGCCAAGAGCGGCTTGCGGAAGC
GGTTGAGCTGGCGGAGGCGAAGCGAGCGGAAGGTCAGCGCGTCGTTTTACAGCATATCACTGGTATTCGCGACATTGACG
CCTACAGTCAGCGCTATCGGTCGATTGTCTATTTGCTTGGCCGGAGCGGGCACGACGGGCAGTAA

Upstream 100 bases:

>100_bases
TTTTCTCTTGACGTAGGAGACAGCAAGGAGTAAACTACTAATAACTTCACTTCTCTACCATATTAGCAAACTAAAGTATT
TAGATAAAGGGCGTGGGGAC

Downstream 100 bases:

>100_bases
ACAGGGCGTCGAAGGAAGCCGGCCGAGTGGCCGAAACGGCGAGAGCGGGGCGCGCTGACAAAGAAAACGTGCACGCGGCG
TGAATGGAATCCAACCCATT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKRLFMFEKPLGMRDTLPFLYEMKKQVRRTMAEEIERWGYEFIETPTLEYYETVGTASAIADHRLFKLLDQQGHTLVLR
PDMTAPIARVAASRLYEDSNPLRLAYNANVFRAQQREGGRPAEFEQIGVELIGDGTVMADAEVISLMAALLKRVGLSHFS
VAVGHIGYVNALFLEILGNEERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLDLLSLRGGSEAIEAAKALVTSEEG
QRAADELAALLAALKTYGVSEAVKLDMALVSHMSYYTGILFEVYAEQVGFPIGNGGRYDDLLAKFSRPAPATGFGLRVDR
LIEAIGETDVRGEIECIVFSQERLAEAVELAEAKRAEGQRVVLQHITGIRDIDAYSQRYRSIVYLLGRSGHDGQ

Sequences:

>Translated_394_residues
MAKRLFMFEKPLGMRDTLPFLYEMKKQVRRTMAEEIERWGYEFIETPTLEYYETVGTASAIADHRLFKLLDQQGHTLVLR
PDMTAPIARVAASRLYEDSNPLRLAYNANVFRAQQREGGRPAEFEQIGVELIGDGTVMADAEVISLMAALLKRVGLSHFS
VAVGHIGYVNALFLEILGNEERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLDLLSLRGGSEAIEAAKALVTSEEG
QRAADELAALLAALKTYGVSEAVKLDMALVSHMSYYTGILFEVYAEQVGFPIGNGGRYDDLLAKFSRPAPATGFGLRVDR
LIEAIGETDVRGEIECIVFSQERLAEAVELAEAKRAEGQRVVLQHITGIRDIDAYSQRYRSIVYLLGRSGHDGQ
>Mature_393_residues
AKRLFMFEKPLGMRDTLPFLYEMKKQVRRTMAEEIERWGYEFIETPTLEYYETVGTASAIADHRLFKLLDQQGHTLVLRP
DMTAPIARVAASRLYEDSNPLRLAYNANVFRAQQREGGRPAEFEQIGVELIGDGTVMADAEVISLMAALLKRVGLSHFSV
AVGHIGYVNALFLEILGNEERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLDLLSLRGGSEAIEAAKALVTSEEGQ
RAADELAALLAALKTYGVSEAVKLDMALVSHMSYYTGILFEVYAEQVGFPIGNGGRYDDLLAKFSRPAPATGFGLRVDRL
IEAIGETDVRGEIECIVFSQERLAEAVELAEAKRAEGQRVVLQHITGIRDIDAYSQRYRSIVYLLGRSGHDGQ

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=391, Percent_Identity=25.3196930946292, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI15029520, Length=387, Percent_Identity=23.5142118863049, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1788861, Length=325, Percent_Identity=25.5384615384615, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71993693, Length=361, Percent_Identity=25.4847645429363, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993686, Length=361, Percent_Identity=25.4847645429363, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=22.1606648199446, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643061, Length=357, Percent_Identity=22.4089635854342, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21

Molecular weight: Translated: 44018; Mature: 43887

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRLFMFEKPLGMRDTLPFLYEMKKQVRRTMAEEIERWGYEFIETPTLEYYETVGTASA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHH
IADHRLFKLLDQQGHTLVLRPDMTAPIARVAASRLYEDSNPLRLAYNANVFRAQQREGGR
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCC
PAEFEQIGVELIGDGTVMADAEVISLMAALLKRVGLSHFSVAVGHIGYVNALFLEILGNE
CCHHHHCCCEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCH
ERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLDLLSLRGGSEAIEAAKALVTSEEG
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
QRAADELAALLAALKTYGVSEAVKLDMALVSHMSYYTGILFEVYAEQVGFPIGNGGRYDD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAKFSRPAPATGFGLRVDRLIEAIGETDVRGEIECIVFSQERLAEAVELAEAKRAEGQR
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHH
VVLQHITGIRDIDAYSQRYRSIVYLLGRSGHDGQ
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKRLFMFEKPLGMRDTLPFLYEMKKQVRRTMAEEIERWGYEFIETPTLEYYETVGTASA
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHH
IADHRLFKLLDQQGHTLVLRPDMTAPIARVAASRLYEDSNPLRLAYNANVFRAQQREGGR
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCC
PAEFEQIGVELIGDGTVMADAEVISLMAALLKRVGLSHFSVAVGHIGYVNALFLEILGNE
CCHHHHCCCEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCH
ERASVLRRFLYEKNYVGYREHVKSLPLSSIDQKRLLDLLSLRGGSEAIEAAKALVTSEEG
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
QRAADELAALLAALKTYGVSEAVKLDMALVSHMSYYTGILFEVYAEQVGFPIGNGGRYDD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAKFSRPAPATGFGLRVDRLIEAIGETDVRGEIECIVFSQERLAEAVELAEAKRAEGQR
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHHHCCCHH
VVLQHITGIRDIDAYSQRYRSIVYLLGRSGHDGQ
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA