Click here to switch to the map view.

The map label for this gene is hpaB [H]

Identifier: 138896629

GI number: 138896629

Start: 3087892

End: 3089370

Strand: Reverse

Name: hpaB [H]

Synonym: GTNG_2992

Alternate gene names: 138896629

Gene position: 3089370-3087892 (Counterclockwise)

Preceding gene: 138896631

Following gene: 138896628

Centisome position: 87.02

GC content: 52.74

Gene sequence:

>1479_bases
ATGCCAGCCAAAACAGGAAATCAGTATATGGAGAGGCTGAAGCAGGCGAAAAGCAGCGTGTACATTCATGGGGAAAAAGT
CGAAGATGTCACCGTCCACCCGGCGTTTCGCAATGTCGTTCGCTCCATGGCGGCGCTGTACGACCGGCAATATGAAAAGC
CGGACAAGATGTTGTATCGGTCGCCGACAACCGGACAGTTGGTCGGGATGACGTTTATCCAGCCGACCACGATCGATGAG
CTCATCGCCCGCCGCGAAGCAACGCAAGAGTGGGCGCGGATGTCGGCCGGAATGATGGGGCGCTCGCCGGATTACTTAAA
CGCCGAAGTGATGGCGATGGGCATCGCCAATGATTTGTTTGCCGAAGACGACCCGATGTTTGCCGAGAATGCGAGAAACT
ATTATGAATATGCGCGGGAAAATGACATCAGCTTGACCCATACGCTCATCCATCCGCAAATGAACCGCGCCAAGGCGCTT
CACGAGCAAAAAGACGCCGATGTGCCGCTCCATTTGGTGGAACGGCGAAAAGACGGCATCATCGTCAGCGGTATCCGTCT
GTTGGCGACGCAAGGTGGAATCACAGATGAAATTTTAGTGTTCCCGTCTACGGTGAAAAAATCGACAGCGGGTGAAGATC
CGTATGCGCTCGCCTTTGCCATCCCGAACAATACGCCGGGGGTGAAGTTCATTTGCCGCGAGGCGTTCGATTACGGGCGC
AGCACGTGGGATCATCCGCTCGCGTCTCGATTTGAAGAAGGCGATGCGATCGTTTCGTTTGAAAACGTGTTTGTGCCATG
GGAGCGCGTCTTTGTTTGCGGCAATTCGTCGATTTGTAACCGGACGTTCCGTGAAACGAATGCGGTCGTTCATATGTCCC
ATCAAGTTGTGGCGAAAAACATCGTCAAAACGGAGTTTTTGCTTGGGGTTACCCTTTGTCTCATTGAAGCAATCGGCATT
GGTGAGTTTCAACATGTGAAGGACAAAGGGGCGGAAATCATGCTTGCTCTCGAAACGATGAAAAGCCACTTGTACCGGGC
TGAGCATAACGCAAAGCCAGATCGTTGGGGAACGATGACACCGGATTTTGCCGCTTTGGATGCTGCGCGCAACTGGTATC
CGCGCGTGTATCCGCGTTTGGTGGAAATCATCCGCATTTTAGGGGCGTCCGGCTTGATGGCGATTCCGACGGAAGCAGAT
TTTGAACATGAAGAAATTGGGGACATCGTCCGCCGGGCGATGCAAGGGGCGACGGTGGACGGTTATGAACGTGTCCAACT
GTTCCGGCTGGCTTGGGATTTGACGATGAGCGCGTTCGGTGCGCGGCAGACGCACTATGAATATTACTTTTTCGGTGACC
CCGTGCGGATGGGGATGGCCTATTTCGACGGCTATGAAAAAGAGTCGTATAAACAGTTTGTGCAAGAATTTTTGCGTACG
AAAAAAAGTGTTTCGATTCCGTTGAACAGCGATCATTGA

Upstream 100 bases:

>100_bases
TGTATTTTTCATATATGAAATCATATGTTATATTAATTTTGAACATTCTGAATGATAGCGCTTACTTCATCTATTATACT
TGAAAATGGGAGGAATGAAA

Downstream 100 bases:

>100_bases
TTAGAGGGGGAGAGGAACAATGACGTTTTCCATCATTCGTTGTGCTCGCGCGGTGCTACATGTGACTGATTTGGCCGCGT
CGCGTGACTTTTACGAACGG

Product: 4-hydroxyphenylacetate-3-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-hydroxylase; 4-hydroxyphenylacetate-3-hydroxylase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MPAKTGNQYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPDKMLYRSPTTGQLVGMTFIQPTTIDE
LIARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLFAEDDPMFAENARNYYEYARENDISLTHTLIHPQMNRAKAL
HEQKDADVPLHLVERRKDGIIVSGIRLLATQGGITDEILVFPSTVKKSTAGEDPYALAFAIPNNTPGVKFICREAFDYGR
STWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKNIVKTEFLLGVTLCLIEAIGI
GEFQHVKDKGAEIMLALETMKSHLYRAEHNAKPDRWGTMTPDFAALDAARNWYPRVYPRLVEIIRILGASGLMAIPTEAD
FEHEEIGDIVRRAMQGATVDGYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKESYKQFVQEFLRT
KKSVSIPLNSDH

Sequences:

>Translated_492_residues
MPAKTGNQYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPDKMLYRSPTTGQLVGMTFIQPTTIDE
LIARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLFAEDDPMFAENARNYYEYARENDISLTHTLIHPQMNRAKAL
HEQKDADVPLHLVERRKDGIIVSGIRLLATQGGITDEILVFPSTVKKSTAGEDPYALAFAIPNNTPGVKFICREAFDYGR
STWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKNIVKTEFLLGVTLCLIEAIGI
GEFQHVKDKGAEIMLALETMKSHLYRAEHNAKPDRWGTMTPDFAALDAARNWYPRVYPRLVEIIRILGASGLMAIPTEAD
FEHEEIGDIVRRAMQGATVDGYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKESYKQFVQEFLRT
KKSVSIPLNSDH
>Mature_491_residues
PAKTGNQYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPDKMLYRSPTTGQLVGMTFIQPTTIDEL
IARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLFAEDDPMFAENARNYYEYARENDISLTHTLIHPQMNRAKALH
EQKDADVPLHLVERRKDGIIVSGIRLLATQGGITDEILVFPSTVKKSTAGEDPYALAFAIPNNTPGVKFICREAFDYGRS
TWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKNIVKTEFLLGVTLCLIEAIGIG
EFQHVKDKGAEIMLALETMKSHLYRAEHNAKPDRWGTMTPDFAALDAARNWYPRVYPRLVEIIRILGASGLMAIPTEADF
EHEEIGDIVRRAMQGATVDGYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKESYKQFVQEFLRTK
KSVSIPLNSDH

Specific function: Utilizes FADH(2) supplied by hpaI, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA) [H]

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FADH(2)-utilizing monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925
- InterPro:   IPR012687 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: 1.14.13.3

Molecular weight: Translated: 55978; Mature: 55847

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKTGNQYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPDKMLYR
CCCCCHHHHHHHHHHHHCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCCCHHHEEC
SPTTGQLVGMTFIQPTTIDELIARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLF
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEHHHHHC
AEDDPMFAENARNYYEYARENDISLTHTLIHPQMNRAKALHEQKDADVPLHLVERRKDGI
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCCCCE
IVSGIRLLATQGGITDEILVFPSTVKKSTAGEDPYALAFAIPNNTPGVKFICREAFDYGR
EEECHHHHHCCCCCCCEEEECCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCC
STWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKN
CCCCCHHHHHHCCCCEEEEECCEECCCEEEEEECCCHHHHHHHHHCCCEEEEHHHHHHHH
IVKTEFLLGVTLCLIEAIGIGEFQHVKDKGAEIMLALETMKSHLYRAEHNAKPDRWGTMT
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
PDFAALDAARNWYPRVYPRLVEIIRILGASGLMAIPTEADFEHEEIGDIVRRAMQGATVD
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCC
GYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKESYKQFVQEFLRT
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHC
KKSVSIPLNSDH
CCCEECCCCCCC
>Mature Secondary Structure 
PAKTGNQYMERLKQAKSSVYIHGEKVEDVTVHPAFRNVVRSMAALYDRQYEKPDKMLYR
CCCCHHHHHHHHHHHHCCEEEECCCCCCEEECHHHHHHHHHHHHHHHHHCCCCHHHEEC
SPTTGQLVGMTFIQPTTIDELIARREATQEWARMSAGMMGRSPDYLNAEVMAMGIANDLF
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEHHHHHC
AEDDPMFAENARNYYEYARENDISLTHTLIHPQMNRAKALHEQKDADVPLHLVERRKDGI
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHCCCCE
IVSGIRLLATQGGITDEILVFPSTVKKSTAGEDPYALAFAIPNNTPGVKFICREAFDYGR
EEECHHHHHCCCCCCCEEEECCCHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCC
STWDHPLASRFEEGDAIVSFENVFVPWERVFVCGNSSICNRTFRETNAVVHMSHQVVAKN
CCCCCHHHHHHCCCCEEEEECCEECCCEEEEEECCCHHHHHHHHHCCCEEEEHHHHHHHH
IVKTEFLLGVTLCLIEAIGIGEFQHVKDKGAEIMLALETMKSHLYRAEHNAKPDRWGTMT
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCC
PDFAALDAARNWYPRVYPRLVEIIRILGASGLMAIPTEADFEHEEIGDIVRRAMQGATVD
CCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCCC
GYERVQLFRLAWDLTMSAFGARQTHYEYYFFGDPVRMGMAYFDGYEKESYKQFVQEFLRT
CHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHC
KKSVSIPLNSDH
CCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA