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The map label for this gene is bphC [H]

Identifier: 138896628

GI number: 138896628

Start: 3086889

End: 3087872

Strand: Reverse

Name: bphC [H]

Synonym: GTNG_2991

Alternate gene names: 138896628

Gene position: 3087872-3086889 (Counterclockwise)

Preceding gene: 138896629

Following gene: 138896627

Centisome position: 86.97

GC content: 54.37

Gene sequence:

>984_bases
ATGACGTTTTCCATCATTCGTTGTGCTCGCGCGGTGCTACATGTGACTGATTTGGCCGCGTCGCGTGACTTTTACGAACG
GGTGCTTGGATTTGTCGTGACGGAAGCGGATCGTGAGCATATCTATTTGCGCGGGTTGGAAGAATACCACCACCATAGCT
TAGTGCTGAAAAAAGCGTCGCGTCCGGCGGTTGAAGCACTTAGCTACAAAGTCGGTTCCGAGCAAGAACTCGAAGCGCTG
TACGAATGGTTTGCGGCGCAACAGCTGAACCCGAGATGGCTTGAGGACGGCTCGCAGCGTGCCATCGGACGGGCGTTTCG
CGTCCAAGACCCATTAGGGCTGCCGCTTGAGTTTTTCGCGCATATGGAGAAAACCGAGCGGTTGTTGCAGCGCTATGACT
TGTATCGCGGTGCCCGCGTCCAACGCATTGACCATTTCAACTGCGCAATAACGGATGTCCAGAAAGGATACGATTTTTAC
GTCCGCGGTTTAGGTTTTGCCTGCTCAGAATATACGGAAACAAAGGATGGCAAGCTGTGGGCGGCTTGGCTGCATCGGAA
GCAAAATGTCCATGATTTGGCGCTCATGAACGGGAAAGGTCCGCGCCTTCATCATATCGGTTTTTGGCTGCCGGATCCGC
TCAGCCTGATTCATGCGTGCGATGTGCTGGCGGCATCGGGATATAGCTCCAGCATTGAACGGGGGCCGGGGCGGCATGGG
TTATCCAATGCGTTCTTTGTCTATTTGCGTGACCCGGACGGCCATCGAATCGAGTTGTATAACGGTGATTATTTGACGAG
CGATCCGGATTTTCCGCCGATTCGCTGGGATTTGGACGATCCGCGGCGCCAGACGTTTTGGGGGCATGCGGCGCCGGACA
GTTGGTTTGAAGAGGCGTCGGACATGTTGGACGTCCATAGCGGCGAACCGATTGAACTGAACGAACCGGCGTTGCAGAAA
GTGAAACCGACCTTTTTGATCTAA

Upstream 100 bases:

>100_bases
AAAGAGTCGTATAAACAGTTTGTGCAAGAATTTTTGCGTACGAAAAAAAGTGTTTCGATTCCGTTGAACAGCGATCATTG
ATTAGAGGGGGAGAGGAACA

Downstream 100 bases:

>100_bases
CAAAAATCATATATGATTTTATAGTTGATTTGATATATATTATCCTGTATAATGTGATTAAGATTCTGAAAAATCAGTCC
ATTAGGAGGCAAGGGGGATG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTFSIIRCARAVLHVTDLAASRDFYERVLGFVVTEADREHIYLRGLEEYHHHSLVLKKASRPAVEALSYKVGSEQELEAL
YEWFAAQQLNPRWLEDGSQRAIGRAFRVQDPLGLPLEFFAHMEKTERLLQRYDLYRGARVQRIDHFNCAITDVQKGYDFY
VRGLGFACSEYTETKDGKLWAAWLHRKQNVHDLALMNGKGPRLHHIGFWLPDPLSLIHACDVLAASGYSSSIERGPGRHG
LSNAFFVYLRDPDGHRIELYNGDYLTSDPDFPPIRWDLDDPRRQTFWGHAAPDSWFEEASDMLDVHSGEPIELNEPALQK
VKPTFLI

Sequences:

>Translated_327_residues
MTFSIIRCARAVLHVTDLAASRDFYERVLGFVVTEADREHIYLRGLEEYHHHSLVLKKASRPAVEALSYKVGSEQELEAL
YEWFAAQQLNPRWLEDGSQRAIGRAFRVQDPLGLPLEFFAHMEKTERLLQRYDLYRGARVQRIDHFNCAITDVQKGYDFY
VRGLGFACSEYTETKDGKLWAAWLHRKQNVHDLALMNGKGPRLHHIGFWLPDPLSLIHACDVLAASGYSSSIERGPGRHG
LSNAFFVYLRDPDGHRIELYNGDYLTSDPDFPPIRWDLDDPRRQTFWGHAAPDSWFEEASDMLDVHSGEPIELNEPALQK
VKPTFLI
>Mature_326_residues
TFSIIRCARAVLHVTDLAASRDFYERVLGFVVTEADREHIYLRGLEEYHHHSLVLKKASRPAVEALSYKVGSEQELEALY
EWFAAQQLNPRWLEDGSQRAIGRAFRVQDPLGLPLEFFAHMEKTERLLQRYDLYRGARVQRIDHFNCAITDVQKGYDFYV
RGLGFACSEYTETKDGKLWAAWLHRKQNVHDLALMNGKGPRLHHIGFWLPDPLSLIHACDVLAASGYSSSIERGPGRHGL
SNAFFVYLRDPDGHRIELYNGDYLTSDPDFPPIRWDLDDPRRQTFWGHAAPDSWFEEASDMLDVHSGEPIELNEPALQKV
KPTFLI

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 37655; Mature: 37524

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSIIRCARAVLHVTDLAASRDFYERVLGFVVTEADREHIYLRGLEEYHHHSLVLKKAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCC
RPAVEALSYKVGSEQELEALYEWFAAQQLNPRWLEDGSQRAIGRAFRVQDPLGLPLEFFA
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHH
HMEKTERLLQRYDLYRGARVQRIDHFNCAITDVQKGYDFYVRGLGFACSEYTETKDGKLW
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHCCCCEEEHHCCHHHHHHCCCCCCCCH
AAWLHRKQNVHDLALMNGKGPRLHHIGFWLPDPLSLIHACDVLAASGYSSSIERGPGRHG
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCC
LSNAFFVYLRDPDGHRIELYNGDYLTSDPDFPPIRWDLDDPRRQTFWGHAAPDSWFEEAS
CCCEEEEEEECCCCCEEEEECCCEECCCCCCCCEEECCCCCHHHCCCCCCCCHHHHHHHH
DMLDVHSGEPIELNEPALQKVKPTFLI
HHHCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TFSIIRCARAVLHVTDLAASRDFYERVLGFVVTEADREHIYLRGLEEYHHHSLVLKKAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHCC
RPAVEALSYKVGSEQELEALYEWFAAQQLNPRWLEDGSQRAIGRAFRVQDPLGLPLEFFA
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHH
HMEKTERLLQRYDLYRGARVQRIDHFNCAITDVQKGYDFYVRGLGFACSEYTETKDGKLW
HHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHCCCCEEEHHCCHHHHHHCCCCCCCCH
AAWLHRKQNVHDLALMNGKGPRLHHIGFWLPDPLSLIHACDVLAASGYSSSIERGPGRHG
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCC
LSNAFFVYLRDPDGHRIELYNGDYLTSDPDFPPIRWDLDDPRRQTFWGHAAPDSWFEEAS
CCCEEEEEEECCCCCEEEEECCCEECCCCCCCCEEECCCCCHHHCCCCCCCCHHHHHHHH
DMLDVHSGEPIELNEPALQKVKPTFLI
HHHCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]