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The map label for this gene is pyrF

Identifier: 138894682

GI number: 138894682

Start: 1080015

End: 1080734

Strand: Direct

Name: pyrF

Synonym: GTNG_1012

Alternate gene names: 138894682

Gene position: 1080015-1080734 (Clockwise)

Preceding gene: 138894681

Following gene: 138894683

Centisome position: 30.42

GC content: 58.33

Gene sequence:

>720_bases
GTGCACACGCCGTTCATTGTCGCTCTTGATTTTCCATCTAAACATGACGTACAGCAGTTTTTACGCCCGTTTAGCGGCAC
GCCGCTGTTTGTGAAAGTCGGCATGGAGCTGTATTATCAGGAGGGTCCAGCCATCATCGCCATGTTCAAAGAACAAGGAC
ATGCCGTGTTTTTAGATTTAAAACTGCACGACATCCCGAATACGGTGAAACAGGCGATGAAAGGGCTCGCTCGCTTAGGT
GTCGATTTAGTGAACGTTCACGCCGCCGGCGGCCGGCGCATGATGGAAGCGGCCATAGAGGGGCTTGACGCTGGAACGCC
AAGCGGCGCTGCTCGGCCGCGCTGCATCGCCGTCACCCAGCTGACCAGCACGGATGAGCGGATGCTTCATGAAGAGCTGT
GGATTTCCCAGCCGCTTGAGGAAACGGTCAGCCATTACGCAGCGTTGGCGCAAGCGAGCGGAATGGACGGTGTCGTCTGC
TCGGCGAACGAAGCGACGCTCATCAAAGAACGGTGCGGTGCTTCGTTTCTCGCGGTCACACCGGGGATTCGCTTTGCCGA
CGATTCAGTGCATGACCAAGTGCGCGTCGTGACGCCAAGGAAAGCACGCGCGCTCGGCGCCGACTACATCGTCATCGGCC
GCAGCATCACCCGCGCCGCTGACCCGCTCGCGGCCTACTCCCGCTTGCAACACGAATGGAACGGAGGAGAAACAGAATGA

Upstream 100 bases:

>100_bases
CCGTTTGTCTGCCCGACGATCATCAATGAACTGCCGGTGCTGCTTGACGAGCTTGGGATTCGACATATCTCGGAATGTAC
GGGAAGGAGCTGGAAAACCG

Downstream 100 bases:

>100_bases
AACACGACATCGCTGCCAAACTGCTTCAGATCGGGGCCGTCGCCCTGCAGCCAAGCGAACCGTTTACGTGGTCGTCTGGC
TTGAAATCGCCCATTTATTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MHTPFIVALDFPSKHDVQQFLRPFSGTPLFVKVGMELYYQEGPAIIAMFKEQGHAVFLDLKLHDIPNTVKQAMKGLARLG
VDLVNVHAAGGRRMMEAAIEGLDAGTPSGAARPRCIAVTQLTSTDERMLHEELWISQPLEETVSHYAALAQASGMDGVVC
SANEATLIKERCGASFLAVTPGIRFADDSVHDQVRVVTPRKARALGADYIVIGRSITRAADPLAAYSRLQHEWNGGETE

Sequences:

>Translated_239_residues
MHTPFIVALDFPSKHDVQQFLRPFSGTPLFVKVGMELYYQEGPAIIAMFKEQGHAVFLDLKLHDIPNTVKQAMKGLARLG
VDLVNVHAAGGRRMMEAAIEGLDAGTPSGAARPRCIAVTQLTSTDERMLHEELWISQPLEETVSHYAALAQASGMDGVVC
SANEATLIKERCGASFLAVTPGIRFADDSVHDQVRVVTPRKARALGADYIVIGRSITRAADPLAAYSRLQHEWNGGETE
>Mature_239_residues
MHTPFIVALDFPSKHDVQQFLRPFSGTPLFVKVGMELYYQEGPAIIAMFKEQGHAVFLDLKLHDIPNTVKQAMKGLARLG
VDLVNVHAAGGRRMMEAAIEGLDAGTPSGAARPRCIAVTQLTSTDERMLHEELWISQPLEETVSHYAALAQASGMDGVVC
SANEATLIKERCGASFLAVTPGIRFADDSVHDQVRVVTPRKARALGADYIVIGRSITRAADPLAAYSRLQHEWNGGETE

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=42.9864253393665, Blast_Score=161, Evalue=3e-41,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_GEOTN (A4IM36)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001125135.1
- ProteinModelPortal:   A4IM36
- SMR:   A4IM36
- STRING:   A4IM36
- GeneID:   4965955
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_1012
- NMPDR:   fig|420246.5.peg.981
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   A4IM36
- ProtClustDB:   PRK00230
- BioCyc:   GTHE420246:GTNG_1012-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26022; Mature: 26022

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 61-61 BINDING 10-10 BINDING 32-32 BINDING 122-122 BINDING 184-184 BINDING 193-193 BINDING 213-213 BINDING 214-214

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTPFIVALDFPSKHDVQQFLRPFSGTPLFVKVGMELYYQEGPAIIAMFKEQGHAVFLDL
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECEEHEEECCCEEEEEEECCCCEEEEEE
KLHDIPNTVKQAMKGLARLGVDLVNVHAAGGRRMMEAAIEGLDAGTPSGAARPRCIAVTQ
EECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
LTSTDERMLHEELWISQPLEETVSHYAALAQASGMDGVVCSANEATLIKERCGASFLAVT
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCEEEEEC
PGIRFADDSVHDQVRVVTPRKARALGADYIVIGRSITRAADPLAAYSRLQHEWNGGETE
CCCEECCCCCCCCEEEECCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHTPFIVALDFPSKHDVQQFLRPFSGTPLFVKVGMELYYQEGPAIIAMFKEQGHAVFLDL
CCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEECEEHEEECCCEEEEEEECCCCEEEEEE
KLHDIPNTVKQAMKGLARLGVDLVNVHAAGGRRMMEAAIEGLDAGTPSGAARPRCIAVTQ
EECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEE
LTSTDERMLHEELWISQPLEETVSHYAALAQASGMDGVVCSANEATLIKERCGASFLAVT
ECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCEEEEEC
PGIRFADDSVHDQVRVVTPRKARALGADYIVIGRSITRAADPLAAYSRLQHEWNGGETE
CCCEECCCCCCCCEEEECCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA