Click here to switch to the map view.

The map label for this gene is liaS [H]

Identifier: 138894115

GI number: 138894115

Start: 493694

End: 494719

Strand: Direct

Name: liaS [H]

Synonym: GTNG_0441

Alternate gene names: 138894115

Gene position: 493694-494719 (Clockwise)

Preceding gene: 138894114

Following gene: 138894116

Centisome position: 13.91

GC content: 50.0

Gene sequence:

>1026_bases
ATGAGCCGACCGTTGATGGTTTCTGTTTTGTCAGCGCTGATTCTTTTCATTTTGTTATCCCTTTCCTTTGTTTACGTGTT
TCCGCCGGAGACGTGGGCGGAGTTGTGGGACAAAACGGTGATGGGCGTACCGTTTTTGCTGTTTGTGTCGGTTGTCAGCT
TATTGATCGGGATCGCGAGCGGACTTGTGTTAGATTTCGTTTGGCGTAAGCAGCGGCAAGTTGTTATGCAGGCGCTCGAG
CAAATTGAACACGGTGAAATGGGGGAACTTTGGCGGGAAGAGCCGTCACCGGAACTGCAGGACCTTTGGCGGCAAATCGA
GAAGCTGCAAACGCAATGGCTCGAGCAAACGAAACGCGTTCAAAAATTAGCGGCAGAAAAGGTCGAACAGGAAGAACGGC
TCGTTGAACGCATTCTTTCCGAAGAACGCACGCGGTTGGCGCGGGAGCTGCATGACTCCGTCAGCCAACAATTGTTTGCC
GCTTCCATGATGATGTCAGCGATTACAGAAACAATGCCACCCGATGATGAACGGCAACGCAAACAATTGAAAATGGTTGA
GCAAATGATTCACCAGTCCCAATTGGAAATGCGGGCGCTGCTTTTGCATTTGCGGCCGGTTCAGCTAAAAGGAAAGTCAC
TGCAAGAAGGGATGGAAGAACTGCTGACAGAATTGACGGGCAAAGTGCCGCTCGAGATGAAATGGAAAATCGAAGATGTT
CCCTTGGATAAAGGGGTGGAAGATCATCTGTTCCGCATTTTGCAAGAGTCGCTTTCCAACACGTTGCGCCATGCAAAAGC
GAAAACGGTTGAAGTGTTGCTGATTGAACGGGACGGGTTTGCAATTTTGCGCGTCACAGACGATGGTGTTGGCTTTGATG
TGGAACGTTCGAAAAGCGGTTCGTACGGGTTGCAGCATATGTATGAACGGGCGGCGGAAATCGGTGGAACATTGAAAGTA
GTCAGCTTAAAAGGCCAAGGAACAAGGTTGGAAGTGAAGGTGCCGCTTGTGTATAAGGGGGGATGA

Upstream 100 bases:

>100_bases
ACCAAACGCTCATTTATCAAACCCCTTCATATCAAGCGGCCGAGAGAAAACTGAAAATTGTCACCTCACTGATGATTGGT
CACCTTGAGGTGAAGCGGTT

Downstream 100 bases:

>100_bases
CCGTGATAAAGGTGTTGCTTGTCGATGATCATGAAATGGTTCGGCTTGGCGTATCCGCGTATTTATCCGCTCAACCGGAC
ATGGAGGTGATCGGCGAGGC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MSRPLMVSVLSALILFILLSLSFVYVFPPETWAELWDKTVMGVPFLLFVSVVSLLIGIASGLVLDFVWRKQRQVVMQALE
QIEHGEMGELWREEPSPELQDLWRQIEKLQTQWLEQTKRVQKLAAEKVEQEERLVERILSEERTRLARELHDSVSQQLFA
ASMMMSAITETMPPDDERQRKQLKMVEQMIHQSQLEMRALLLHLRPVQLKGKSLQEGMEELLTELTGKVPLEMKWKIEDV
PLDKGVEDHLFRILQESLSNTLRHAKAKTVEVLLIERDGFAILRVTDDGVGFDVERSKSGSYGLQHMYERAAEIGGTLKV
VSLKGQGTRLEVKVPLVYKGG

Sequences:

>Translated_341_residues
MSRPLMVSVLSALILFILLSLSFVYVFPPETWAELWDKTVMGVPFLLFVSVVSLLIGIASGLVLDFVWRKQRQVVMQALE
QIEHGEMGELWREEPSPELQDLWRQIEKLQTQWLEQTKRVQKLAAEKVEQEERLVERILSEERTRLARELHDSVSQQLFA
ASMMMSAITETMPPDDERQRKQLKMVEQMIHQSQLEMRALLLHLRPVQLKGKSLQEGMEELLTELTGKVPLEMKWKIEDV
PLDKGVEDHLFRILQESLSNTLRHAKAKTVEVLLIERDGFAILRVTDDGVGFDVERSKSGSYGLQHMYERAAEIGGTLKV
VSLKGQGTRLEVKVPLVYKGG
>Mature_340_residues
SRPLMVSVLSALILFILLSLSFVYVFPPETWAELWDKTVMGVPFLLFVSVVSLLIGIASGLVLDFVWRKQRQVVMQALEQ
IEHGEMGELWREEPSPELQDLWRQIEKLQTQWLEQTKRVQKLAAEKVEQEERLVERILSEERTRLARELHDSVSQQLFAA
SMMMSAITETMPPDDERQRKQLKMVEQMIHQSQLEMRALLLHLRPVQLKGKSLQEGMEELLTELTGKVPLEMKWKIEDVP
LDKGVEDHLFRILQESLSNTLRHAKAKTVEVLLIERDGFAILRVTDDGVGFDVERSKSGSYGLQHMYERAAEIGGTLKVV
SLKGQGTRLEVKVPLVYKGG

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=199, Percent_Identity=28.643216080402, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787474, Length=207, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082311, Length=229, Percent_Identity=31.8777292576419, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39139; Mature: 39008

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPLMVSVLSALILFILLSLSFVYVFPPETWAELWDKTVMGVPFLLFVSVVSLLIGIAS
CCCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLDFVWRKQRQVVMQALEQIEHGEMGELWREEPSPELQDLWRQIEKLQTQWLEQTKRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QKLAAEKVEQEERLVERILSEERTRLARELHDSVSQQLFAASMMMSAITETMPPDDERQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KQLKMVEQMIHQSQLEMRALLLHLRPVQLKGKSLQEGMEELLTELTGKVPLEMKWKIEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PLDKGVEDHLFRILQESLSNTLRHAKAKTVEVLLIERDGFAILRVTDDGVGFDVERSKSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCC
SYGLQHMYERAAEIGGTLKVVSLKGQGTRLEVKVPLVYKGG
CHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCC
>Mature Secondary Structure 
SRPLMVSVLSALILFILLSLSFVYVFPPETWAELWDKTVMGVPFLLFVSVVSLLIGIAS
CCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVLDFVWRKQRQVVMQALEQIEHGEMGELWREEPSPELQDLWRQIEKLQTQWLEQTKRV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QKLAAEKVEQEERLVERILSEERTRLARELHDSVSQQLFAASMMMSAITETMPPDDERQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
KQLKMVEQMIHQSQLEMRALLLHLRPVQLKGKSLQEGMEELLTELTGKVPLEMKWKIEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PLDKGVEDHLFRILQESLSNTLRHAKAKTVEVLLIERDGFAILRVTDDGVGFDVERSKSG
CCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCEEEEEEECCCCCCCCCCCCCC
SYGLQHMYERAAEIGGTLKVVSLKGQGTRLEVKVPLVYKGG
CHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]