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The map label for this gene is spaK [H]

Identifier: 138893946

GI number: 138893946

Start: 307208

End: 308626

Strand: Direct

Name: spaK [H]

Synonym: GTNG_0270

Alternate gene names: 138893946

Gene position: 307208-308626 (Clockwise)

Preceding gene: 138893945

Following gene: 138893955

Centisome position: 8.65

GC content: 37.28

Gene sequence:

>1419_bases
ATGAAAAAGAATAAAACGATTAAACGAGATTTTTATTTGTTAGTTGTGAAAGTCGTGTTGGCAACGGCAATCTCTTCGGC
CGTTACTTATTTATGCTTAATCTATTTGATTATTACATTAGCCACGAACGATGTGGTGAAACCGACAAATTATTTCGTGA
AGGATTTGGATTTGATTGAAAAAAAGGTGAAAGAAAACGAAGAGGCGATTTTCAAGGGGAGATTAATTCCCATCCATCGT
TACAGTGAAAAGATACAGGGAGAAGTTGTAGACATCAGCGGTAAGCATCTGTATGGTGATCGAGAAATTATAGACGATCA
AGTGGATATTAGTCAATCGATCAACCGTGAGATTGTAAAAGGAAGCTATGTGTATCGGTTCATTCCCTTGAAGCATGATA
ACGCCATTCAGGCGGTATATGTGTTGAAAGCGCCTTTTGGGTTCATCATTAACAATCAAAATCGCTTCGAAGCCGTGTTG
ATTTATGCTGTGATGTTTATTTCTCCGTTGATCTTTTTTATTGTCTATTTGATTTTCTTTACATCTAGGCTGTACAAATC
ATTGTTTCATAATGTCAAACTGTTGCTCCAGGCTTCGGATCACATTGCAAGCGGGAATTTTGATTTTCAAGTCTCGGGGT
TGAAAAGAAAAGAGTTTATCAAGATCCAGGATTCCTTTAATACCATGATCAGTGCACTGAAAGAAATGGTGGAACGTTTA
GCAAAGACCGATGACGAACGAAAAATGATGGTATCATCGATCGCACATGATATCAGAACCCCGTTAACGGTCATCCAAGG
ACAAATTGATTTGATTCAAGATTTGAGAAAGTTGGACCACTTTGATGTCACTCCCCATTTAGAGATTATTCGCAAAAACT
GTGGGAAGATGACCATGTTGACAGATAATTTGTCGTTGCTCTATAAAGTGGAGAATGACCACTTCTCATTGAATGGAAAA
GAAGTGGATGTAAGGCAAATCCTTGAGGAGAAAAAACTAGAGATCTCAACAATGGTTTACCATCAAAAATCGATAAACAT
TTGCTTTGATGTGAATTTGCAAAAATCACGTTACATACTTGATGAAGCGATGTTAATGAGAGTGTTGGACAATATTTTAT
ACAACAGCCTGAGGTTTACAAAAAGCGGAGAAATTAAGTTGGAAGTCTATGATGAAAAAGAGGGGAATGAGTGTAAAATC
TATTTTCGCTGTTCAGATACAGGAACGGGGTTTAAAGAAAACGATACGTCTTTGTTGTTCCAGCCTTTTTACCAGGATAA
GCAGTATAAAAATCATGTTGGATTAGGACTGTATATTGCCAAACGAATTGTGAATCATCATGGCGGTGAAATTTGGGCCT
ACAACAATGAAAAAGGCGGAGCGACGGTTGAATTTTATATTAAGGAGCTATCTGATTAA

Upstream 100 bases:

>100_bases
GAAAGCATTAAAAATATTCGAAAGAAAATCAAGGCCGTCGATCCAGAAACCTCTTATATTCAAACATTATATGGGCTCGG
TTATAAATGGGATGTGACCG

Downstream 100 bases:

>100_bases
TCATCCAATCTTATGGGCTTTCCGCCGTTATAGAGAGTGGAGAATGCAGCTAGGGAGGAATTTCTCTTTCCCTAGCTGAC
AGTCTCTAAGCCTTTATTTT

Product: sensory transduction protein kinase SpaK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDVVKPTNYFVKDLDLIEKKVKENEEAIFKGRLIPIHR
YSEKIQGEVVDISGKHLYGDREIIDDQVDISQSINREIVKGSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVL
IYAVMFISPLIFFIVYLIFFTSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKIQDSFNTMISALKEMVERL
AKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHFDVTPHLEIIRKNCGKMTMLTDNLSLLYKVENDHFSLNGK
EVDVRQILEEKKLEISTMVYHQKSINICFDVNLQKSRYILDEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECKI
YFRCSDTGTGFKENDTSLLFQPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEKGGATVEFYIKELSD

Sequences:

>Translated_472_residues
MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDVVKPTNYFVKDLDLIEKKVKENEEAIFKGRLIPIHR
YSEKIQGEVVDISGKHLYGDREIIDDQVDISQSINREIVKGSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVL
IYAVMFISPLIFFIVYLIFFTSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKIQDSFNTMISALKEMVERL
AKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHFDVTPHLEIIRKNCGKMTMLTDNLSLLYKVENDHFSLNGK
EVDVRQILEEKKLEISTMVYHQKSINICFDVNLQKSRYILDEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECKI
YFRCSDTGTGFKENDTSLLFQPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEKGGATVEFYIKELSD
>Mature_472_residues
MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDVVKPTNYFVKDLDLIEKKVKENEEAIFKGRLIPIHR
YSEKIQGEVVDISGKHLYGDREIIDDQVDISQSINREIVKGSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVL
IYAVMFISPLIFFIVYLIFFTSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKIQDSFNTMISALKEMVERL
AKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHFDVTPHLEIIRKNCGKMTMLTDNLSLLYKVENDHFSLNGK
EVDVRQILEEKKLEISTMVYHQKSINICFDVNLQKSRYILDEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECKI
YFRCSDTGTGFKENDTSLLFQPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEKGGATVEFYIKELSD

Specific function: Member of the two-component regulatory system resD/resE involved in the global regulation of aerobic and anaerobic respiration. Probably phosphorylates resD [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=27.1777003484321, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789149, Length=361, Percent_Identity=24.0997229916898, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786912, Length=241, Percent_Identity=24.896265560166, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI145693157, Length=278, Percent_Identity=25.5395683453237, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786600, Length=216, Percent_Identity=27.3148148148148, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=22.8, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790551, Length=243, Percent_Identity=24.2798353909465, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081816, Length=341, Percent_Identity=22.5806451612903, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788549, Length=255, Percent_Identity=22.3529411764706, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=23.6734693877551, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082128, Length=244, Percent_Identity=25, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54779; Mature: 54779

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDVVKPTNYFVKDLDLIE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KKVKENEEAIFKGRLIPIHRYSEKIQGEVVDISGKHLYGDREIIDDQVDISQSINREIVK
HHHHCCCHHHHCCCEEEHHHHHHHHCCEEEEECCCEECCCHHHCCCHHHHHHHHHHHHHC
GSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVLIYAVMFISPLIFFIVYLIFF
CCEEEEEEEECCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKIQDSFNTMISALKEMVERL
HHHHHHHHHHHHHEEEEECCCEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
AKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHFDVTPHLEIIRKNCGKMTML
HCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEE
TDNLSLLYKVENDHFSLNGKEVDVRQILEEKKLEISTMVYHQKSINICFDVNLQKSRYIL
ECCCEEEEEEECCEEECCCCEEHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCHHHHHH
DEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECKIYFRCSDTGTGFKENDTSLLF
HHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEE
QPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEKGGATVEFYIKELSD
ECHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDVVKPTNYFVKDLDLIE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
KKVKENEEAIFKGRLIPIHRYSEKIQGEVVDISGKHLYGDREIIDDQVDISQSINREIVK
HHHHCCCHHHHCCCEEEHHHHHHHHCCEEEEECCCEECCCHHHCCCHHHHHHHHHHHHHC
GSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVLIYAVMFISPLIFFIVYLIFF
CCEEEEEEEECCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKIQDSFNTMISALKEMVERL
HHHHHHHHHHHHHEEEEECCCEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHH
AKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHFDVTPHLEIIRKNCGKMTML
HCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEE
TDNLSLLYKVENDHFSLNGKEVDVRQILEEKKLEISTMVYHQKSINICFDVNLQKSRYIL
ECCCEEEEEEECCEEECCCCEEHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCHHHHHH
DEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECKIYFRCSDTGTGFKENDTSLLF
HHHHHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEE
QPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEKGGATVEFYIKELSD
ECHHCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 8631715 [H]