Click here to switch to the map view.

The map label for this gene is spaR [H]

Identifier: 138893945

GI number: 138893945

Start: 306523

End: 307221

Strand: Direct

Name: spaR [H]

Synonym: GTNG_0269

Alternate gene names: 138893945

Gene position: 306523-307221 (Clockwise)

Preceding gene: 138893944

Following gene: 138893946

Centisome position: 8.63

GC content: 37.2

Gene sequence:

>699_bases
ATGAATAACAAGAAAATTTTGGTCGTTGATGATGATGAAGATATTTGCTGCACCATTAAACAATATTTGGAGCTTCATCA
TTATCAGGTAGAAACGGTCCACAGCGGCGTAGAAGCGTTGAAGATGATAAACGACGATTTTGATTTGATTGTGTTGGATA
TCATGATGGAAGGAATTGACGGAATGGAGCTGTGTACAATCATTCGTGATCGGGTTGATTACCCTATTGTATTTGTTAGT
GCTAAAACGCTGGAGGAAGATAAGGTTCAAGCTCTGGCGATCGGTGGGGATGATTACATCACCAAACCGTTCAGCTTAAA
GGAGTTAAAGGCGAGAATTGATTCTCATTTAAGAAGGGAAGAACGGATTCGTTCAAACGAGAGATATCTTTTGAGTTCTA
AAAATATTACGATTGATCTTTTGGCTAACGAAGTCTTTTGTAAAGGAAAGAAATTACCGTTAACAAAAAAAGAATATGAG
ATTGTTAAGTTGTTGATGCTCAATAAAAAGGTCGTTTTCTCCAAAGAAAGAATCTTTGAACGAGTATGGGGGATTGACTC
CGACAGCCAGTTAGACACTGTTACAGAAAGCATTAAAAATATTCGAAAGAAAATCAAGGCCGTCGATCCAGAAACCTCTT
ATATTCAAACATTATATGGGCTCGGTTATAAATGGGATGTGACCGATGAAAAAGAATAA

Upstream 100 bases:

>100_bases
CATTGCACCTATGAACAAACGCAACGGTGTTATGAGATTACCCCATGATATGGGGATGGAATAGGTTAAAATGCGATTCA
ACTGTATGGGGGATCAAAAG

Downstream 100 bases:

>100_bases
AACGATTAAACGAGATTTTTATTTGTTAGTTGTGAAAGTCGTGTTGGCAACGGCAATCTCTTCGGCCGTTACTTATTTAT
GCTTAATCTATTTGATTATT

Product: subtilin biosynthesis regulatory protein SpaR

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMINDDFDLIVLDIMMEGIDGMELCTIIRDRVDYPIVFVS
AKTLEEDKVQALAIGGDDYITKPFSLKELKARIDSHLRREERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYE
IVKLLMLNKKVVFSKERIFERVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLGYKWDVTDEKE

Sequences:

>Translated_232_residues
MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMINDDFDLIVLDIMMEGIDGMELCTIIRDRVDYPIVFVS
AKTLEEDKVQALAIGGDDYITKPFSLKELKARIDSHLRREERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYE
IVKLLMLNKKVVFSKERIFERVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLGYKWDVTDEKE
>Mature_232_residues
MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMINDDFDLIVLDIMMEGIDGMELCTIIRDRVDYPIVFVS
AKTLEEDKVQALAIGGDDYITKPFSLKELKARIDSHLRREERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYE
IVKLLMLNKKVVFSKERIFERVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLGYKWDVTDEKE

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=230, Percent_Identity=35.2173913043478, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=32.15859030837, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=30.6306306306306, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=29.7777777777778, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=28.3842794759825, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=30.7017543859649, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=27.3542600896861, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789402, Length=221, Percent_Identity=28.0542986425339, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI2367329, Length=224, Percent_Identity=31.25, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=28.4444444444444, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=27.1929824561404, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693140, Length=228, Percent_Identity=25.8771929824561, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790437, Length=135, Percent_Identity=29.6296296296296, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26971; Mature: 26971

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMINDDFDLIVLDIMMEGID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GMELCTIIRDRVDYPIVFVSAKTLEEDKVQALAIGGDDYITKPFSLKELKARIDSHLRRE
HHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYEIVKLLMLNKKVVFSKERIFE
HHHCCCCEEEEECCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
RVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLGYKWDVTDEKE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCCCC
>Mature Secondary Structure
MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMINDDFDLIVLDIMMEGID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GMELCTIIRDRVDYPIVFVSAKTLEEDKVQALAIGGDDYITKPFSLKELKARIDSHLRRE
HHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYEIVKLLMLNKKVVFSKERIFE
HHHCCCCEEEEECCCEEEEEECCHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
RVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLGYKWDVTDEKE
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]