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The map label for this gene is purB [H]

Identifier: 138893915

GI number: 138893915

Start: 266759

End: 268054

Strand: Direct

Name: purB [H]

Synonym: GTNG_0239

Alternate gene names: 138893915

Gene position: 266759-268054 (Clockwise)

Preceding gene: 138893914

Following gene: 138893916

Centisome position: 7.51

GC content: 50.39

Gene sequence:

>1296_bases
ATGATTGAACGCTACACAAGACCCGAAATGGGAGCCATTTGGACAGAGGAAAACCGCTTTAAGGCGTGGCTTGAGGTGGA
ATTGCTTGCGTGCGAGGCGTGGGCCGAGCTTGGTGTCATTCCGAAAGAAGATGTCCGCCGCCTACGGGAAAACGCGTCAT
TTGACATTCATCGCATTAAAGAAATCGAGGAAGAAACGCGCCATGATGTTGTCGCGTTCACCCGCGCTGTTTCTGAGACG
CTTGGCGAAGAGCGCAAGTGGGTACACTACGGGCTTACATCGACAGACGTCGTCGACACGGCGCTCGGCTATTTATTAAA
GCAGGCGAACACGATTTTGAGGCGCGATTTAGAAAACTTTATCCAAGTGCTGAAAGAAAAAGCGCTTGAACATAAATATA
CGGTCATGATGGGGCGCACGCATGGCGTTCATGCGGAACCGACGACGTTCGGATTAAAACTGGCGCTCTGGTATGCGGAA
ATGAAACGGAATTTGGAGCGGTTCGAGCAAGCAGCGAAGATGGTCGAAGTCGGGAAAATTTCCGGCGCTGTTGGAACGTA
TGCCAACATTGATCCATTTGTCGAACAATATGTGTGCGAGAAGCTCGGGTTAACGCCAGCGCCAATTTCGACGCAAACGC
TCCAGCGCGACCGCCACGCTTACTACATGGCTACGCTCGCACTCATTGCGACATCGATTGAAAAATTCGCCGTTGAGATT
CGCGGTTTGCAAAAAAGCGAAGTGCGCGAAGTCGAGGAGTTTTTCGCCAAGGGGCAAAAAGGCTCATCAGCGATGCCGCA
TAAGCGCAACCCGATCGGCTCGGAAAACATGACCGGCATGGCACGCGTCATTCGTGGGTATATGGTGACGGCTTATGAAA
ATGTGCCGCTTTGGCATGAACGTGACATTTCCCACTCGTCAGCTGAGCGCATCATTTTACCGGATGCGACGATCGCACTG
AACTATATGTTGAACCGATTCGCTAACATTGTGAAAAACTTGCTTGTGTATCCGGAAAATATGAAGAAAAATATGGAGCG
CACATTCGGCCTCATTTACTCACAACGCGTCTTGCTCGCTTTGATCGACAAAGGGATGACGCGTGAAGCAGCGTACGATT
TAGTGCAGCCGAAGGCGATGGAAGCGTGGGAAAAGCAAGTTCCGTTCCGCTCTCTTCTCGAAGCGGATGAGCGGATTACA
AGCCAGTTGACGAAAGAAGAACTCGACGATTGCTTTGATTACCGCCACCATTTGAAACATGTTGATACGATTTTTGCCCG
CTTAGGATTAGAGTAA

Upstream 100 bases:

>100_bases
GAAAGATGGGCCATGTGACCGTGTTGGCGGACAATGTGGATGAGGCGCTGGCGAGATTGGAGTCGTGGCAAATTTGGCAT
GATCGGAGGCTAGAACGAGT

Downstream 100 bases:

>100_bases
GTGACAACCGGCTGGTTCTAATGCCGGCCGGTTACACAAAAAATTTTCAATATTCTGTACCACGGAGGCTTGGATGATGC
CGACAAAACAACAGCTGCTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTRPEMGAIWTEENRFKAWLEVELLACEAWAELGVIPKEDVRRLRENASFDIHRIKEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALGYLLKQANTILRRDLENFIQVLKEKALEHKYTVMMGRTHGVHAEPTTFGLKLALWYAE
MKRNLERFEQAAKMVEVGKISGAVGTYANIDPFVEQYVCEKLGLTPAPISTQTLQRDRHAYYMATLALIATSIEKFAVEI
RGLQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVPLWHERDISHSSAERIILPDATIAL
NYMLNRFANIVKNLLVYPENMKKNMERTFGLIYSQRVLLALIDKGMTREAAYDLVQPKAMEAWEKQVPFRSLLEADERIT
SQLTKEELDDCFDYRHHLKHVDTIFARLGLE

Sequences:

>Translated_431_residues
MIERYTRPEMGAIWTEENRFKAWLEVELLACEAWAELGVIPKEDVRRLRENASFDIHRIKEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALGYLLKQANTILRRDLENFIQVLKEKALEHKYTVMMGRTHGVHAEPTTFGLKLALWYAE
MKRNLERFEQAAKMVEVGKISGAVGTYANIDPFVEQYVCEKLGLTPAPISTQTLQRDRHAYYMATLALIATSIEKFAVEI
RGLQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVPLWHERDISHSSAERIILPDATIAL
NYMLNRFANIVKNLLVYPENMKKNMERTFGLIYSQRVLLALIDKGMTREAAYDLVQPKAMEAWEKQVPFRSLLEADERIT
SQLTKEELDDCFDYRHHLKHVDTIFARLGLE
>Mature_431_residues
MIERYTRPEMGAIWTEENRFKAWLEVELLACEAWAELGVIPKEDVRRLRENASFDIHRIKEIEEETRHDVVAFTRAVSET
LGEERKWVHYGLTSTDVVDTALGYLLKQANTILRRDLENFIQVLKEKALEHKYTVMMGRTHGVHAEPTTFGLKLALWYAE
MKRNLERFEQAAKMVEVGKISGAVGTYANIDPFVEQYVCEKLGLTPAPISTQTLQRDRHAYYMATLALIATSIEKFAVEI
RGLQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVPLWHERDISHSSAERIILPDATIAL
NYMLNRFANIVKNLLVYPENMKKNMERTFGLIYSQRVLLALIDKGMTREAAYDLVQPKAMEAWEKQVPFRSLLEADERIT
SQLTKEELDDCFDYRHHLKHVDTIFARLGLE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=396, Percent_Identity=28.2828282828283, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI183227688, Length=398, Percent_Identity=28.894472361809, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1787376, Length=305, Percent_Identity=27.8688524590164, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=411, Percent_Identity=26.2773722627737, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=371, Percent_Identity=26.1455525606469, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=29.4871794871795, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24647570, Length=393, Percent_Identity=25.9541984732824, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49749; Mature: 49749

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTRPEMGAIWTEENRFKAWLEVELLACEAWAELGVIPKEDVRRLRENASFDIHRIK
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHH
EIEEETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTALGYLLKQANTILRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQVLKEKALEHKYTVMMGRTHGVHAEPTTFGLKLALWYAEMKRNLERFEQAAKMVEVGKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPFVEQYVCEKLGLTPAPISTQTLQRDRHAYYMATLALIATSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVPLWHE
HCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCC
RDISHSSAERIILPDATIALNYMLNRFANIVKNLLVYPENMKKNMERTFGLIYSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGMTREAAYDLVQPKAMEAWEKQVPFRSLLEADERITSQLTKEELDDCFDYRHHLKH
HHHCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDTIFARLGLE
HHHHHHHHCCC
>Mature Secondary Structure
MIERYTRPEMGAIWTEENRFKAWLEVELLACEAWAELGVIPKEDVRRLRENASFDIHRIK
CCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHH
EIEEETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTALGYLLKQANTILRRDLENF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQVLKEKALEHKYTVMMGRTHGVHAEPTTFGLKLALWYAEMKRNLERFEQAAKMVEVGKI
HHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIDPFVEQYVCEKLGLTPAPISTQTLQRDRHAYYMATLALIATSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGLQKSEVREVEEFFAKGQKGSSAMPHKRNPIGSENMTGMARVIRGYMVTAYENVPLWHE
HCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEECCCCCCCC
RDISHSSAERIILPDATIALNYMLNRFANIVKNLLVYPENMKKNMERTFGLIYSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LIDKGMTREAAYDLVQPKAMEAWEKQVPFRSLLEADERITSQLTKEELDDCFDYRHHLKH
HHHCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDTIFARLGLE
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]