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The map label for this gene is phoB [H]

Identifier: 13474926

GI number: 13474926

Start: 4779921

End: 4780625

Strand: Direct

Name: phoB [H]

Synonym: mlr5913

Alternate gene names: 13474926

Gene position: 4779921-4780625 (Clockwise)

Preceding gene: 13474925

Following gene: 13474927

Centisome position: 67.93

GC content: 62.7

Gene sequence:

>705_bases
ATGAAGCCACGCGTCCTGATCTGTTCGCAAGATGCGGAGTTCTATCTGTTCCTCAGCCACATACTGGAGGTGGACGGGTT
CGTCAGTGAGCCGGCAGGCGGTGCGAAGGAAGCACTTGCAATGGCCGACGATCGCGACTTCCAGGCCGTGGTGCTGGACT
GCGGTTCAACGAGCCTTACCGGGTCCGCAATCTGCGCCCGGCTCAAGCGGGAGCCCCGAACCGGCGGCCTGCCCATCATT
GCCCTGATCGCGCCCGGCGCCGAGAACCAGCACCTCGATCTGTTGAAGGCCGGCATTGACGAGAGCTTTGTGCGGCCTGT
GGCGCCGGCCAAGCTGCTCGATTGTCTGCGGACGAGGCTGGCGCTGCCGAAGCCGGGTTCAAACGCGATTGAAAACAACA
GCTGGCTTTCCTATGGCAGCCTTGAGATGAAGCTCGATGCCCACCGGGTTTGCGGCAATGGCCATGACATCCATCTCGGA
CCGATCGAGTTCAACGTGCTGCGGCATTTGCTCGAGGCGCCCGGCAAGGTCTTCAGTAGGGACGAGCTGATCGGCGGGGC
CTGGCCGGCCAATATCCATGTCGGTGTACGCACAGTCGACGTCCACATCAGTCGGCTCAGAAGGGCTCTGGAGGCGGCCT
CGACGGGTATCGTCATCCGTACCGTCAGGTCGGCCGGCTACTCGCTCGAGAAGCTGGACGGCTGA

Upstream 100 bases:

>100_bases
ATTTTCGAGCAAGAGGGTCATGTCAGGCCTTGAGGAGCCGCGATGGCCAGCCCACGCGGCCGACGCGGTCGAGGCCAATT
TGGTTCTCGGGGTAGCGCTG

Downstream 100 bases:

>100_bases
ATTGAGTGCCGAGTGTGGTTCCACTCCCTTTCCTCGCGCTGTGAGCCGGTCTTGCGGCCTGAAACGCCCTCAACGGATAC
TCAGGAAGAAACAGGAAAAT

Product: phosphate regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKPRVLICSQDAEFYLFLSHILEVDGFVSEPAGGAKEALAMADDRDFQAVVLDCGSTSLTGSAICARLKREPRTGGLPII
ALIAPGAENQHLDLLKAGIDESFVRPVAPAKLLDCLRTRLALPKPGSNAIENNSWLSYGSLEMKLDAHRVCGNGHDIHLG
PIEFNVLRHLLEAPGKVFSRDELIGGAWPANIHVGVRTVDVHISRLRRALEAASTGIVIRTVRSAGYSLEKLDG

Sequences:

>Translated_234_residues
MKPRVLICSQDAEFYLFLSHILEVDGFVSEPAGGAKEALAMADDRDFQAVVLDCGSTSLTGSAICARLKREPRTGGLPII
ALIAPGAENQHLDLLKAGIDESFVRPVAPAKLLDCLRTRLALPKPGSNAIENNSWLSYGSLEMKLDAHRVCGNGHDIHLG
PIEFNVLRHLLEAPGKVFSRDELIGGAWPANIHVGVRTVDVHISRLRRALEAASTGIVIRTVRSAGYSLEKLDG
>Mature_234_residues
MKPRVLICSQDAEFYLFLSHILEVDGFVSEPAGGAKEALAMADDRDFQAVVLDCGSTSLTGSAICARLKREPRTGGLPII
ALIAPGAENQHLDLLKAGIDESFVRPVAPAKLLDCLRTRLALPKPGSNAIENNSWLSYGSLEMKLDAHRVCGNGHDIHLG
PIEFNVLRHLLEAPGKVFSRDELIGGAWPANIHVGVRTVDVHISRLRRALEAASTGIVIRTVRSAGYSLEKLDG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=29.2576419213974, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=28.0701754385965, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790860, Length=217, Percent_Identity=29.4930875576037, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=26.2008733624454, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=25.2212389380531, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=25.2136752136752, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=226, Percent_Identity=26.5486725663717, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=207, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25247; Mature: 25247

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRVLICSQDAEFYLFLSHILEVDGFVSEPAGGAKEALAMADDRDFQAVVLDCGSTSLT
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCC
GSAICARLKREPRTGGLPIIALIAPGAENQHLDLLKAGIDESFVRPVAPAKLLDCLRTRL
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHH
ALPKPGSNAIENNSWLSYGSLEMKLDAHRVCGNGHDIHLGPIEFNVLRHLLEAPGKVFSR
CCCCCCCCCCCCCCCEEECCEEEEEEHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCC
DELIGGAWPANIHVGVRTVDVHISRLRRALEAASTGIVIRTVRSAGYSLEKLDG
CCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEEHHCCCCHHHCCC
>Mature Secondary Structure
MKPRVLICSQDAEFYLFLSHILEVDGFVSEPAGGAKEALAMADDRDFQAVVLDCGSTSLT
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCC
GSAICARLKREPRTGGLPIIALIAPGAENQHLDLLKAGIDESFVRPVAPAKLLDCLRTRL
HHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHH
ALPKPGSNAIENNSWLSYGSLEMKLDAHRVCGNGHDIHLGPIEFNVLRHLLEAPGKVFSR
CCCCCCCCCCCCCCCEEECCEEEEEEHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCC
DELIGGAWPANIHVGVRTVDVHISRLRRALEAASTGIVIRTVRSAGYSLEKLDG
CCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEEEHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]