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The map label for this gene is nifN

Identifier: 13474923

GI number: 13474923

Start: 4777474

End: 4778856

Strand: Direct

Name: nifN

Synonym: mlr5909

Alternate gene names: 13474923

Gene position: 4777474-4778856 (Clockwise)

Preceding gene: 13474922

Following gene: 13474924

Centisome position: 67.9

GC content: 60.59

Gene sequence:

>1383_bases
ATGGTCCGCATCCTTCGCAAAATCAAATCAGCGGCGGTCAACCCGCTGAAGTCGTCGCAGCCGCTGGGTGCCGCCTTCGC
TTTTCTTGGAGTCGAGGGTGCGATGCCCCTGTTCCACGGCAGCCAAGGCTGCACCAGCTTCGCGCTCGTGCTCTTCGTGC
GGCATTTCAAGGAAGCGATCCCCTTGCAGACAACGGCGATGGATGAGGTGGCAACCATACTCGGCGGAGCGGACCATCTG
GAAGAGGCGATCCTCAACCTCAAAACCCGCACGAAGCCAAAGCTGATAGGAGTGTGCACTACCGCGCTGGTGGAGACCCG
TGGCGAAGACTGCGCGAGTGATATCGCCAACGTCAAACTGAAGCACGTGGAAGAGCTCGCGGGTACGGAGGTCGTGCTGG
CCAACACGCCTGATTTTGACGGCGCGATCGAAGAAGGCTGGGCCAAGGCCGTCGCGGCGATGATCGAAGGGATTACACGG
TCGGGTGAACGGACGCGGCAGCCGAAGAAGATCGCGATCCTGCCCGGATGCAACCTCACTGTCGCTGACGTCGAGCATTT
GCGTGACATGGTTGAAAGTTTTGGGCTCAAGCCGGTTATCCTGCCGGACGTCTCCGGCTCACTCGACGGCACTGTTCCTG
ACCGCTGGGTAACCACTACCTATGGCGGCACCAGCGTCGAGGAAATCCGCGAGCTTGGCACGGCCGCGCAATGCATCGTC
ATCGGCGAGCACATGCGCCACCCGGCGAAAACGCTGCACGGGTTGACCGGCGTGCCTTACGCGGTGTTCCAGTCGCTGAC
CGGATTAAAGGCCGTCGACCGGTTCGTCTCGCTGCTATCGGCGGTTTCGGGCGCGGCGGTGCCGGACCGGGTTCGCCGTC
ACCGGGCGCAATTGGAGGATGCATTGCTCGACGGACATTTCCATTTCGGAGGCAAGAAAATTGCGATCGCTGCTGAACCA
GACCAACTCTATCAACTCGCAACCTTCTTCACCGGCATGGGCTGTGACATCGCGGCGGCGGTCACGACGACCGATATGTC
GAAGATTCTGCAAAAAGTACCGGCGGAGTGGGTTCAGATCGGCGATCTCGGCGATTTGGAAGCTCTTGCAGCGGGCGCGG
ATCTTCTCGTAACACATTCCCACGGTCGCCAGGCGTCGCGACGCCTTGAAATTCCGCTCATGCGCGTCGGCTTCCCGATC
TTCGACCGGCTCGGCAGCCAGCACAAGCTCACAATCCTCTATCGGGGGACCCGCGACCTGATCTTCGATGTTGCCAATAT
CTTTCAGGCCAACCAGCACGCGCCGACGCCTGAGGCGCTTGATCCATTCCGGAAACGAGAAATGCCAGATGAGCTCCGTT
CGTCGCCTCTCACTCGTCACTGA

Upstream 100 bases:

>100_bases
CGATCTGGCGAGCGCGAAGAACGGGACAGCGACTGTGAACGCTTTCAATGAATTCGCCGGCGCGATGGCTCACCGCTTCG
GCGACCGATGAGGAAATTCG

Downstream 100 bases:

>100_bases
CGAGGTTCACGCACCGATGACGGACCGACGGGCAGGCGCATTGCGCGTAGCCATCGCCACGCAAGACATGACGAATCTCA
ATGCCCATTTCGGGTCGGCC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MVRILRKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAIPLQTTAMDEVATILGGADHL
EEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKLKHVEELAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITR
SGERTRQPKKIAILPGCNLTVADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIV
IGEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLEDALLDGHFHFGGKKIAIAAEP
DQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQIGDLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPI
FDRLGSQHKLTILYRGTRDLIFDVANIFQANQHAPTPEALDPFRKREMPDELRSSPLTRH

Sequences:

>Translated_460_residues
MVRILRKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAIPLQTTAMDEVATILGGADHL
EEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKLKHVEELAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITR
SGERTRQPKKIAILPGCNLTVADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIV
IGEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLEDALLDGHFHFGGKKIAIAAEP
DQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQIGDLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPI
FDRLGSQHKLTILYRGTRDLIFDVANIFQANQHAPTPEALDPFRKREMPDELRSSPLTRH
>Mature_460_residues
MVRILRKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAIPLQTTAMDEVATILGGADHL
EEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKLKHVEELAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITR
SGERTRQPKKIAILPGCNLTVADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIV
IGEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLEDALLDGHFHFGGKKIAIAAEP
DQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQIGDLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPI
FDRLGSQHKLTILYRGTRDLIFDVANIFQANQHAPTPEALDPFRKREMPDELRSSPLTRH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor)

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFN_RHILO (Q98AP3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106493.1
- ProteinModelPortal:   Q98AP3
- SMR:   Q98AP3
- GeneID:   1229152
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5909
- NMPDR:   fig|266835.1.peg.4596
- HOGENOM:   HBG336444
- OMA:   CTSFGLV
- ProtClustDB:   PRK14476
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975
- TIGRFAMs:   TIGR01285

Pfam domain/function: PF00148 Oxidored_nitro

EC number: NA

Molecular weight: Translated: 49806; Mature: 49806

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRILRKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAI
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
PLQTTAMDEVATILGGADHLEEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
KHVEELAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITRSGERTRQPKKIAILPGCNLT
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCEE
VADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIV
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCHHHEEE
IGEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLED
ECHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALLDGHFHFGGKKIAIAAEPDQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQI
HHHCCEEEECCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEC
GDLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPIFDRLGSQHKLTILYRGTRDL
CCCCCHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHCCHHHHHCCCCCEEEEEEECCHHH
IFDVANIFQANQHAPTPEALDPFRKREMPDELRSSPLTRH
HHHHHHHHHCCCCCCCCHHHCHHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure
MVRILRKIKSAAVNPLKSSQPLGAAFAFLGVEGAMPLFHGSQGCTSFALVLFVRHFKEAI
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
PLQTTAMDEVATILGGADHLEEAILNLKTRTKPKLIGVCTTALVETRGEDCASDIANVKL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
KHVEELAGTEVVLANTPDFDGAIEEGWAKAVAAMIEGITRSGERTRQPKKIAILPGCNLT
HHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCEE
VADVEHLRDMVESFGLKPVILPDVSGSLDGTVPDRWVTTTYGGTSVEEIRELGTAAQCIV
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCHHHEEE
IGEHMRHPAKTLHGLTGVPYAVFQSLTGLKAVDRFVSLLSAVSGAAVPDRVRRHRAQLED
ECHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ALLDGHFHFGGKKIAIAAEPDQLYQLATFFTGMGCDIAAAVTTTDMSKILQKVPAEWVQI
HHHCCEEEECCEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHEEC
GDLGDLEALAAGADLLVTHSHGRQASRRLEIPLMRVGFPIFDRLGSQHKLTILYRGTRDL
CCCCCHHHHHCCCCEEEECCCCHHHHHHCCCCHHHHCCHHHHHCCCCCEEEEEEECCHHH
IFDVANIFQANQHAPTPEALDPFRKREMPDELRSSPLTRH
HHHHHHHHHCCCCCCCCHHHCHHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968