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The map label for this gene is nifE

Identifier: 13474922

GI number: 13474922

Start: 4775974

End: 4777464

Strand: Direct

Name: nifE

Synonym: mlr5908

Alternate gene names: 13474922

Gene position: 4775974-4777464 (Clockwise)

Preceding gene: 13474921

Following gene: 13474923

Centisome position: 67.88

GC content: 62.17

Gene sequence:

>1491_bases
ATGACCTCGCTCAGTGCCAAGATCCAGGACGTTTTCGACGAGCCCGCCTGCGATACCAACCGCGGCAAGGATGCCAAGGC
GCGCAAGGAGGGCTGCTCGAAGCCGCTGACCCCCGGGGCGTCAGCCGGCGGCTGCGCCTTTGACGGCGCCAAGATCGTCC
TGCAGCCGATCACCGACGTTGCGCACCTGGTCCATGCGCCGCTCGCCTGCGAGGGCAATTCCTGGGACAACAGAGGCGCA
GCTTCGTCCGGGCCAACCCTGTGGCGGACAAGCTTCACAACAGACCTCACCGAACTCGACATCGTGATGGGGCAGGGCGA
GCGGAAGCTATTCAAGGCGATCCGCGAGATCAAGGAAACATATGCGCCGCCGGCAGTCTTCGTCTATTCGACCTGCGTGA
CGGCGCTGATCGGCGACGACATCGAGGCCGTGTGCAAACGCGCGGCCGAAAAATTCGGACTGGCCGTGGTGCCGATCAAT
GCGCCGGGCCTGGCCGGCTCCAAGAACCTCGGCAACAAACTCGCCGCCGAAGCGTTGCTCGATCATGTCATCGGCACGGT
GGAACCCGACGACGCCGGGCCCTACGACATCAACATCCTTGGCGAATTCAACCTCTCCGGCGAATTCTGGCTGGTCAAGC
CGCTGCTTGATCGGCTTGGCATCCGGGTGCGCGCCTGCATACCGGGCGATGCGCGTTACATCGACGTTGCATCCGCGCAC
CGCGCCCGGGCGGCTATGATGGTGTGCTCGAGCGCACTGATCAATCTGGCCCGCAAGATGGAGGAGCGCTGGGACATCCC
GTACTTCGAGGGCTCTTTCTACGGCATAACCGATACCTCGGAAGCACTTCGCAACATTGCTGAGCTGCTGGTGAGGAAGG
GCGCCGATGCGGAGATCCTCGATCGCACGGAGACGCTGATTGCTGAGCAGGAGGCGATTGCGTGGAAGAAGCTCGAGGCC
TACCGCCGGCGGCTTCAAGGCAAGCGCGTGCTGCTCAACACGGGCGGTGTGAAGTCCTGGTCGGTCGTCCACGCGCTGAT
GGAGATCGGCATGGAGATCGTCGGCACCTCGGTCAAGAAATCCACAGTCGAAGACAAGGAGCGGATCAAACGGATCCTCA
AGGACGAAAACCACATGTTCGAGCAGATGGCAGCGCGCGATCTCTACGCCATGCTTTCAAAACACAAGGCCGACATCATG
CTGTCGGGCGGGCGTACGCAATTCGTCGCCCTGAAGGCCAAGACACCCTGGCTCGATATCAACCAGGAGCGCCAACATCC
TTATGCCGGCTATGACGGCATGGTGGAACTTGTCCGGCAGATTGACCTCGCCATTCATAACCCGATCTGGGGTCAGGTGC
GGGAGCCGGCGCCATGGGATTGCCAGCCTGCCAGGGAGGACGATCTGGCGAGCGCGAAGAACGGGACAGCGACTGTGAAC
GCTTTCAATGAATTCGCCGGCGCGATGGCTCACCGCTTCGGCGACCGATGA

Upstream 100 bases:

>100_bases
AAACAGGCGTGACGGACTATTCCTATGACCTCACCCGCTAAGCCCGTGCCGGCCTTTCGCCGAGGCCGCCCATGCCCAAT
GGACTTCGGAGACTGACGCA

Downstream 100 bases:

>100_bases
GGAAATTCGATGGTCCGCATCCTTCGCAAAATCAAATCAGCGGCGGTCAACCCGCTGAAGTCGTCGCAGCCGCTGGGTGC
CGCCTTCGCTTTTCTTGGAG

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTSLSAKIQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGA
ASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKETYAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVVPIN
APGLAGSKNLGNKLAAEALLDHVIGTVEPDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAH
RARAAMMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEILDRTETLIAEQEAIAWKKLEA
YRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKKSTVEDKERIKRILKDENHMFEQMAARDLYAMLSKHKADIM
LSGGRTQFVALKAKTPWLDINQERQHPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWDCQPAREDDLASAKNGTATVN
AFNEFAGAMAHRFGDR

Sequences:

>Translated_496_residues
MTSLSAKIQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGA
ASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKETYAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVVPIN
APGLAGSKNLGNKLAAEALLDHVIGTVEPDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAH
RARAAMMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEILDRTETLIAEQEAIAWKKLEA
YRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKKSTVEDKERIKRILKDENHMFEQMAARDLYAMLSKHKADIM
LSGGRTQFVALKAKTPWLDINQERQHPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWDCQPAREDDLASAKNGTATVN
AFNEFAGAMAHRFGDR
>Mature_495_residues
TSLSAKIQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGAA
SSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKETYAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVVPINA
PGLAGSKNLGNKLAAEALLDHVIGTVEPDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAHR
ARAAMMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEILDRTETLIAEQEAIAWKKLEAY
RRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKKSTVEDKERIKRILKDENHMFEQMAARDLYAMLSKHKADIML
SGGRTQFVALKAKTPWLDINQERQHPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWDCQPAREDDLASAKNGTATVNA
FNEFAGAMAHRFGDR

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor)

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFE_RHILO (Q98AP4)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106492.1
- ProteinModelPortal:   Q98AP4
- SMR:   Q98AP4
- GeneID:   1229151
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5908
- NMPDR:   fig|266835.1.peg.4595
- HOGENOM:   HBG392742
- OMA:   YTWDIRG
- ProtClustDB:   PRK14478
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973
- TIGRFAMs:   TIGR01283

Pfam domain/function: PF00148 Oxidored_nitro

EC number: NA

Molecular weight: Translated: 54439; Mature: 54308

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSAKIQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDV
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHAPLACEGNSWDNRGAASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKET
HHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHEECCCHHHHHHHHHHHHHH
YAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVVPINAPGLAGSKNLGNKLAAEALL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHH
DHVIGTVEPDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAH
HHHHCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHCCCEEEEECCCCCCEEECHHHH
RARAAMMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCHHHH
DRTETLIAEQEAIAWKKLEAYRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
STVEDKERIKRILKDENHMFEQMAARDLYAMLSKHKADIMLSGGRTQFVALKAKTPWLDI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCC
NQERQHPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWDCQPAREDDLASAKNGTATVN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEH
AFNEFAGAMAHRFGDR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSLSAKIQDVFDEPACDTNRGKDAKARKEGCSKPLTPGASAGGCAFDGAKIVLQPITDV
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHAPLACEGNSWDNRGAASSGPTLWRTSFTTDLTELDIVMGQGERKLFKAIREIKET
HHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHEECCCHHHHHHHHHHHHHH
YAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVVPINAPGLAGSKNLGNKLAAEALL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHHHHHHH
DHVIGTVEPDDAGPYDINILGEFNLSGEFWLVKPLLDRLGIRVRACIPGDARYIDVASAH
HHHHCCCCCCCCCCEEEEEEEEECCCCCEEEHHHHHHHCCCEEEEECCCCCCEEECHHHH
RARAAMMVCSSALINLARKMEERWDIPYFEGSFYGITDTSEALRNIAELLVRKGADAEIL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCHHHH
DRTETLIAEQEAIAWKKLEAYRRRLQGKRVLLNTGGVKSWSVVHALMEIGMEIVGTSVKK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
STVEDKERIKRILKDENHMFEQMAARDLYAMLSKHKADIMLSGGRTQFVALKAKTPWLDI
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEEECCCCCCC
NQERQHPYAGYDGMVELVRQIDLAIHNPIWGQVREPAPWDCQPAREDDLASAKNGTATVN
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCEEEH
AFNEFAGAMAHRFGDR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968