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The map label for this gene is nifK1 [H]

Identifier: 13474921

GI number: 13474921

Start: 4774373

End: 4775914

Strand: Direct

Name: nifK1 [H]

Synonym: mlr5907

Alternate gene names: 13474921

Gene position: 4774373-4775914 (Clockwise)

Preceding gene: 13474920

Following gene: 13474922

Centisome position: 67.86

GC content: 59.4

Gene sequence:

>1542_bases
ATGCCGCAATCGGCCGAAAAAGTTCTCGACCATGCTCCCCTGTTCCGCGAGCCGGAATACAGACAAATGCTTGCGGAGAA
GAAGCTCAATTTCGAATGTCCGCACCCTGATCAGATCGTCACCGACCAACGCGAATTGACCAAGACTTGGGAATACCGCG
AAAAGAACCTCGCTCGCGAGGCGCTTGTCATAAATCCCGCCAAGGCCTGCCAGCCGCTCGGCGCGGTGTTCGCGGCCGCG
GGCTTCGAGCGCACCATGTCTTTCGTCCACGGTAGCCAGGGCTGCGTCGCCTACTACCGCTCGCATCTGTCGCGACATTT
CAAGGAGCCTTCGTCAGCGGTTTCCTCCTCGATGACCGAGGACGCGGCAGTGTTTGGCGGCCTGAAGAACATGGTCGACG
GACTCGCCAATACCTACAAGCTCTACGACCCCAAGATGATCGCCGTCTCGACCACCTGCATGGCTGAAGTCATTGGCGAC
GACCTGCGGAGCTTCATCGAGAACGCCAAGAACGAAGGCTCGGTCCCGAGCGACTTCGACGTGCCTTTCGCGCATACGCC
TGCCTTCGTCGGCAGCCATATCGATGGCTATGACGGCATGGTGAAGGGCGTGCTGGAGCATTTCTGGAAGGGCAGCGAGC
GCTCGCAAGCCGCTGGGACCATCAACATCATTCCAGGCTTCGACGGATTCTGCGTCGGAAACAACCGAGAACTCAAGCGC
CTCCTCGACCTGATGGGTGTCACTTATACCGTCATTCAAGACGCCTCAGACCAGTTCGACACGCCGTCTGACGGCGAATA
CCGCATGTATGACGGTGGCACGAAGATCGAAGACTTGAAAGGCGCACTCAACGCGGAGGCGACACTGTCGCTGCAGCATT
ACAACACCCGCAAGACGCTGGAATATTGCGACGAGGTCGGGCAAGCGACGGCATCCTTCCACTATCCGCTGGGTGTCCAG
GCGACCGACGAATTCCTGATGAAGGTCTCGGCGATTTCCGGCCAGGAGATCCCCGAGGCAATCCGGATGGAGCGCGGCCG
ACTGGTTGACGCCATGGCGGACAGCCAGTCATGGCTGCACGGCAAGAAGTACGCAATCTATGGGGATCCGGACTTCGTCT
ACGCCACGGCCCGCTTCGTCATGGAGACCGGTGGCGAGCCGACACATTGCCTCGCGACCAACGGGACATCGGCCTGGGAG
GCCGAGATGAAGGAATTGCTTGCATCCTCGTCCTTCGGCCAGGATGCCCAGATCTGGGCGGGCAAGGATCTCTGGGCGAT
GCGTTCGCTGCTGTTTACAGAGCCGGTGGACCTGCTGATCGGCAATTCCTATGGCAAGTATCTGGAGCGCGACACCGGCA
CGCCGCTGATCCGGCTGATGTTTCCAATCTTCGACCGGCACCATCACCATCGCTTTCCCCTCATGGGCTACCAGGGTGGA
TTACGCGTGCTGACGACGATCCTCGACAAGATATTCGACAAGCTCGATCGCGAGACGAGCGAAACAGGCGTGACGGACTA
TTCCTATGACCTCACCCGCTAA

Upstream 100 bases:

>100_bases
ATAAATGCTTGGGGCGTGCTCGATCCACGCTGGATGCCCTTCAAAGTTTCGATGTCCCTGTGCTGCCGCTTGCCGCGGCC
AGATGGAAAAGGTGACCACC

Downstream 100 bases:

>100_bases
GCCCGTGCCGGCCTTTCGCCGAGGCCGCCCATGCCCAATGGACTTCGGAGACTGACGCAATGACCTCGCTCAGTGCCAAG
ATCCAGGACGTTTTCGACGA

Product: nitrogenase molybdenum-iron protein beta chain, nifK

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPQSAEKVLDHAPLFREPEYRQMLAEKKLNFECPHPDQIVTDQRELTKTWEYREKNLAREALVINPAKACQPLGAVFAAA
GFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGD
DLRSFIENAKNEGSVPSDFDVPFAHTPAFVGSHIDGYDGMVKGVLEHFWKGSERSQAAGTINIIPGFDGFCVGNNRELKR
LLDLMGVTYTVIQDASDQFDTPSDGEYRMYDGGTKIEDLKGALNAEATLSLQHYNTRKTLEYCDEVGQATASFHYPLGVQ
ATDEFLMKVSAISGQEIPEAIRMERGRLVDAMADSQSWLHGKKYAIYGDPDFVYATARFVMETGGEPTHCLATNGTSAWE
AEMKELLASSSFGQDAQIWAGKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
LRVLTTILDKIFDKLDRETSETGVTDYSYDLTR

Sequences:

>Translated_513_residues
MPQSAEKVLDHAPLFREPEYRQMLAEKKLNFECPHPDQIVTDQRELTKTWEYREKNLAREALVINPAKACQPLGAVFAAA
GFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGD
DLRSFIENAKNEGSVPSDFDVPFAHTPAFVGSHIDGYDGMVKGVLEHFWKGSERSQAAGTINIIPGFDGFCVGNNRELKR
LLDLMGVTYTVIQDASDQFDTPSDGEYRMYDGGTKIEDLKGALNAEATLSLQHYNTRKTLEYCDEVGQATASFHYPLGVQ
ATDEFLMKVSAISGQEIPEAIRMERGRLVDAMADSQSWLHGKKYAIYGDPDFVYATARFVMETGGEPTHCLATNGTSAWE
AEMKELLASSSFGQDAQIWAGKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
LRVLTTILDKIFDKLDRETSETGVTDYSYDLTR
>Mature_512_residues
PQSAEKVLDHAPLFREPEYRQMLAEKKLNFECPHPDQIVTDQRELTKTWEYREKNLAREALVINPAKACQPLGAVFAAAG
FERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTEDAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDD
LRSFIENAKNEGSVPSDFDVPFAHTPAFVGSHIDGYDGMVKGVLEHFWKGSERSQAAGTINIIPGFDGFCVGNNRELKRL
LDLMGVTYTVIQDASDQFDTPSDGEYRMYDGGTKIEDLKGALNAEATLSLQHYNTRKTLEYCDEVGQATASFHYPLGVQA
TDEFLMKVSAISGQEIPEAIRMERGRLVDAMADSQSWLHGKKYAIYGDPDFVYATARFVMETGGEPTHCLATNGTSAWEA
EMKELLASSSFGQDAQIWAGKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGGL
RVLTTILDKIFDKLDRETSETGVTDYSYDLTR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 57296; Mature: 57165

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSAEKVLDHAPLFREPEYRQMLAEKKLNFECPHPDQIVTDQRELTKTWEYREKNLARE
CCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHH
ALVINPAKACQPLGAVFAAAGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTE
HEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
DAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLRSFIENAKNEGSVPSDFD
HHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPFAHTPAFVGSHIDGYDGMVKGVLEHFWKGSERSQAAGTINIIPGFDGFCVGNNRELKR
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCHHHHH
LLDLMGVTYTVIQDASDQFDTPSDGEYRMYDGGTKIEDLKGALNAEATLSLQHYNTRKTL
HHHHHCCHHHHHHCCHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCCCHHHH
EYCDEVGQATASFHYPLGVQATDEFLMKVSAISGQEIPEAIRMERGRLVDAMADSQSWLH
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHCCHHHHC
GKKYAIYGDPDFVYATARFVMETGGEPTHCLATNGTSAWEAEMKELLASSSFGQDAQIWA
CCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEC
GKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
CCHHHHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
LRVLTTILDKIFDKLDRETSETGVTDYSYDLTR
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PQSAEKVLDHAPLFREPEYRQMLAEKKLNFECPHPDQIVTDQRELTKTWEYREKNLARE
CCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHH
ALVINPAKACQPLGAVFAAAGFERTMSFVHGSQGCVAYYRSHLSRHFKEPSSAVSSSMTE
HEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
DAAVFGGLKNMVDGLANTYKLYDPKMIAVSTTCMAEVIGDDLRSFIENAKNEGSVPSDFD
HHHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPFAHTPAFVGSHIDGYDGMVKGVLEHFWKGSERSQAAGTINIIPGFDGFCVGNNRELKR
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEECCCHHHHH
LLDLMGVTYTVIQDASDQFDTPSDGEYRMYDGGTKIEDLKGALNAEATLSLQHYNTRKTL
HHHHHCCHHHHHHCCHHCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCCCHHHH
EYCDEVGQATASFHYPLGVQATDEFLMKVSAISGQEIPEAIRMERGRLVDAMADSQSWLH
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHCCHHHHC
GKKYAIYGDPDFVYATARFVMETGGEPTHCLATNGTSAWEAEMKELLASSSFGQDAQIWA
CCEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEC
GKDLWAMRSLLFTEPVDLLIGNSYGKYLERDTGTPLIRLMFPIFDRHHHHRFPLMGYQGG
CCHHHHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCH
LRVLTTILDKIFDKLDRETSETGVTDYSYDLTR
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]