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The map label for this gene is dut

Identifier: 13474457

GI number: 13474457

Start: 4269053

End: 4269538

Strand: Reverse

Name: dut

Synonym: mll5345

Alternate gene names: 13474457

Gene position: 4269538-4269053 (Counterclockwise)

Preceding gene: 13474458

Following gene: 13474456

Centisome position: 60.68

GC content: 65.64

Gene sequence:

>486_bases
ATGCGCGCAGCTCTCCAAAACTCCTCTGTCATCGGTCCGACTGTCGGCTTCGTCAGATTGCCGCATGCTGAAGGACTTCC
CCTCCCCGCCTATGAAAGCACCGGCGCCGCCGGCATGGATCTTCGCGCCGCGGTACCCGACGACCGGCCGCTGCTGATCC
TGCCTGGGAAACGCTCCTTGGTGCCGACCGGGCTGATCCTGGAGATCCCCGAAGGCATGGAAGGTCAGGTGCGGCCGCGC
TCCGGCCTTGCTTTCAAGCACGGCCTGACCGTCCTCAATTCCCCCGGCACGGTCGACAGCGACTATCGCGGCGAGGTGAA
GGTGCTTTTGATCAATCTCGGCGACGAGGATTTCGCGGTGACGCGCGGTATGCGCATCGCCCAGATCGTTTTCGCTGTTG
TGACGCAGGCGGCCGTCGAGGAGCGTTCGCTGGCCGGCGGCACGGCACGCGGTTCGGGCGGGTTCGGATCGACCGGCACC
GTCTGA

Upstream 100 bases:

>100_bases
CTAAAGCGTGCTCAGGTCATAGTGATGCCTGAAAGAAAGCCGTTGATAGCGGCGCGGGCGAATTGACCGAATCGGCAATC
GCGCCGATAGGATCGATTTT

Downstream 100 bases:

>100_bases
TGCAAATACCCAAGCTTGTGATCTTCGACTGCGACGGGATTCTCGTCGACACGGAGAATCTCGCCAATCGACGCCTCGCT
GAATGGTTGACGGCTGCCGG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MRAALQNSSVIGPTVGFVRLPHAEGLPLPAYESTGAAGMDLRAAVPDDRPLLILPGKRSLVPTGLILEIPEGMEGQVRPR
SGLAFKHGLTVLNSPGTVDSDYRGEVKVLLINLGDEDFAVTRGMRIAQIVFAVVTQAAVEERSLAGGTARGSGGFGSTGT
V

Sequences:

>Translated_161_residues
MRAALQNSSVIGPTVGFVRLPHAEGLPLPAYESTGAAGMDLRAAVPDDRPLLILPGKRSLVPTGLILEIPEGMEGQVRPR
SGLAFKHGLTVLNSPGTVDSDYRGEVKVLLINLGDEDFAVTRGMRIAQIVFAVVTQAAVEERSLAGGTARGSGGFGSTGT
V
>Mature_161_residues
MRAALQNSSVIGPTVGFVRLPHAEGLPLPAYESTGAAGMDLRAAVPDDRPLLILPGKRSLVPTGLILEIPEGMEGQVRPR
SGLAFKHGLTVLNSPGTVDSDYRGEVKVLLINLGDEDFAVTRGMRIAQIVFAVVTQAAVEERSLAGGTARGSGGFGSTGT
V

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=143, Percent_Identity=42.6573426573427, Blast_Score=97, Evalue=5e-21,
Organism=Homo sapiens, GI4503423, Length=152, Percent_Identity=41.4473684210526, Blast_Score=97, Evalue=6e-21,
Organism=Homo sapiens, GI70906441, Length=143, Percent_Identity=42.6573426573427, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1790071, Length=135, Percent_Identity=41.4814814814815, Blast_Score=104, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71988561, Length=146, Percent_Identity=44.5205479452055, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=38.8059701492537, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921126, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=40.9090909090909, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_RHILO (Q98C10)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106025.1
- ProteinModelPortal:   Q98C10
- SMR:   Q98C10
- GeneID:   1228686
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5345
- NMPDR:   fig|266835.1.peg.4129
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16718; Mature: 16718

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: BINDING 93-93 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAALQNSSVIGPTVGFVRLPHAEGLPLPAYESTGAAGMDLRAAVPDDRPLLILPGKRSL
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCC
VPTGLILEIPEGMEGQVRPRSGLAFKHGLTVLNSPGTVDSDYRGEVKVLLINLGDEDFAV
CCCCEEEECCCCCCCCCCCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEH
TRGMRIAQIVFAVVTQAAVEERSLAGGTARGSGGFGSTGTV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAALQNSSVIGPTVGFVRLPHAEGLPLPAYESTGAAGMDLRAAVPDDRPLLILPGKRSL
CCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCCCC
VPTGLILEIPEGMEGQVRPRSGLAFKHGLTVLNSPGTVDSDYRGEVKVLLINLGDEDFAV
CCCCEEEECCCCCCCCCCCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEH
TRGMRIAQIVFAVVTQAAVEERSLAGGTARGSGGFGSTGTV
HCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968