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The map label for this gene is puuA [H]

Identifier: 13474292

GI number: 13474292

Start: 4084205

End: 4085605

Strand: Reverse

Name: puuA [H]

Synonym: mll5148

Alternate gene names: 13474292

Gene position: 4085605-4084205 (Counterclockwise)

Preceding gene: 13474293

Following gene: 13474291

Centisome position: 58.07

GC content: 65.67

Gene sequence:

>1401_bases
ATGGACAATGCCATCGTGACCTCCCCTTCGGGGTCCTCGCCCGCCGAGGCGCAAGCCTTTCTCGACGCCCATCCCGAGAT
CGAAGCCTTCGATATCGTGCTGACCGACGCCAATGGCGTGGGCCGCGGCAAGATCGTACGCCGGCATGAGCTGAAGAGCA
TTTTCGAGGGTGGCCGGCATATGCCGATCTCGATCCTCGGCCTCGACATTACCGGCGAGGACGTGCACGAGACCGGACTG
ATCTGGACGACAGGCGACGGCGACCTCCGGGCATGGCCAATCCCCGGCACGCTGGTGCCGCTCTACGGCACCAATCCACC
GCGTGGCCAGGTGCTGATGGCGATGTATCATCTCGACGGCCAGCCCATGTCGTCGGACCCGCGCCTCGCGCTCGCCCGGC
AGGTCGACATCCTCGCGGCCAAGGGGCTTTACCCGGCGGGCGCCTTCGAACTGGAATTCTTCCTGCTGGCCAACGAGCGC
GACGCCGACGGCAAGGTGCAGCCGGCGCGCGCGGTGCTCGATGGCCGCGTTTCGGGCAAGACGGAGGTCTATTCAGTCGA
CCATCTGCACGGCATGGAGCCGCTGTTCTCCGACATTTACGCCGCCGCCAAGGCGCAAGGCATTCCGGCCGAGACGGTGA
TATCGGAATATGCGCCCGGCCAGTACGAGCTGACGCTGAACTACCGCAAGGATGTGATGCGGGCCGCCGACGACCTCGTC
ATGCTGAAGCGGCTGGTGCGGGCGCAGGCGCGCCGCCATGGCGTCACCGCCTGCTTCATGGCCAAGCCGATCGAGAAATA
TGCCGGCTCGGGCATGCATTTCCACGTCTCGCTGCAGGACAAAGCCGGCCGCAACGTCTTTGCCGAAGCCGGCGGCGAGA
GCTGGTCGCTACCCTTGCTGCAGGGGCTCGGCGGGCTGATCCAGACGATGGCGGAATCAATGCTGGTGTTTGCGCCGCAC
GCCAATTCCTGGCGGCGATTCGTCTCGCAATCCTATGCGCCGGTGGCGCCAACCTGGGGCGTCAACAATCGCTCGGTGGC
GCTGCGCGTGCCGGCGGGCGACGCCAAGAACCGGCGCATCGAGCACCGGCCGTCGGGCGTCGACGCCAATCCCTACCTGA
TCGCCGCGACGGTGCTGGCCGGCATCGTCAAAGGCCTCGACGAAGGGCTCGATCCTGGTCCAGAAACGACCGGCAATGGC
TATGAGGCGGCCGTCACCCGCACGACGATGCCGGCGGACTGGCGCGCGGCGATCGAAGCGGCCAAGGCCTCCAGCTTCCT
CAAAGGCGCGCTGGGCGAGGATTTGCACCGCACCTTCGTGGCGATCAAACAGTCCGAATATCTGCGTGTGGCGCGCACCG
TCAGCGAACTGGATTATCATCTCTATTTGCACGAGGTGTGA

Upstream 100 bases:

>100_bases
TGATGCTGATCTCGCCGGCGACGTCAGGCGCCCAGGTCAACCGGTTGATCGCCGCCTTCGGCGCGGTTGCGGCGAAGCTT
GCGGCGTGAGACAAGCGGCC

Downstream 100 bases:

>100_bases
GGATCGGGAACCATCATCCGCCACGTCCATTTCCTGTCGAAATGGACGAGACAGATGACAGCAATCCTGCCGCACCGAAC
GCAGACATCCGCGGGACGCG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MDNAIVTSPSGSSPAEAQAFLDAHPEIEAFDIVLTDANGVGRGKIVRRHELKSIFEGGRHMPISILGLDITGEDVHETGL
IWTTGDGDLRAWPIPGTLVPLYGTNPPRGQVLMAMYHLDGQPMSSDPRLALARQVDILAAKGLYPAGAFELEFFLLANER
DADGKVQPARAVLDGRVSGKTEVYSVDHLHGMEPLFSDIYAAAKAQGIPAETVISEYAPGQYELTLNYRKDVMRAADDLV
MLKRLVRAQARRHGVTACFMAKPIEKYAGSGMHFHVSLQDKAGRNVFAEAGGESWSLPLLQGLGGLIQTMAESMLVFAPH
ANSWRRFVSQSYAPVAPTWGVNNRSVALRVPAGDAKNRRIEHRPSGVDANPYLIAATVLAGIVKGLDEGLDPGPETTGNG
YEAAVTRTTMPADWRAAIEAAKASSFLKGALGEDLHRTFVAIKQSEYLRVARTVSELDYHLYLHEV

Sequences:

>Translated_466_residues
MDNAIVTSPSGSSPAEAQAFLDAHPEIEAFDIVLTDANGVGRGKIVRRHELKSIFEGGRHMPISILGLDITGEDVHETGL
IWTTGDGDLRAWPIPGTLVPLYGTNPPRGQVLMAMYHLDGQPMSSDPRLALARQVDILAAKGLYPAGAFELEFFLLANER
DADGKVQPARAVLDGRVSGKTEVYSVDHLHGMEPLFSDIYAAAKAQGIPAETVISEYAPGQYELTLNYRKDVMRAADDLV
MLKRLVRAQARRHGVTACFMAKPIEKYAGSGMHFHVSLQDKAGRNVFAEAGGESWSLPLLQGLGGLIQTMAESMLVFAPH
ANSWRRFVSQSYAPVAPTWGVNNRSVALRVPAGDAKNRRIEHRPSGVDANPYLIAATVLAGIVKGLDEGLDPGPETTGNG
YEAAVTRTTMPADWRAAIEAAKASSFLKGALGEDLHRTFVAIKQSEYLRVARTVSELDYHLYLHEV
>Mature_466_residues
MDNAIVTSPSGSSPAEAQAFLDAHPEIEAFDIVLTDANGVGRGKIVRRHELKSIFEGGRHMPISILGLDITGEDVHETGL
IWTTGDGDLRAWPIPGTLVPLYGTNPPRGQVLMAMYHLDGQPMSSDPRLALARQVDILAAKGLYPAGAFELEFFLLANER
DADGKVQPARAVLDGRVSGKTEVYSVDHLHGMEPLFSDIYAAAKAQGIPAETVISEYAPGQYELTLNYRKDVMRAADDLV
MLKRLVRAQARRHGVTACFMAKPIEKYAGSGMHFHVSLQDKAGRNVFAEAGGESWSLPLLQGLGGLIQTMAESMLVFAPH
ANSWRRFVSQSYAPVAPTWGVNNRSVALRVPAGDAKNRRIEHRPSGVDANPYLIAATVLAGIVKGLDEGLDPGPETTGNG
YEAAVTRTTMPADWRAAIEAAKASSFLKGALGEDLHRTFVAIKQSEYLRVARTVSELDYHLYLHEV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=421, Percent_Identity=24.7030878859857, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87081870, Length=441, Percent_Identity=40.3628117913832, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1790301, Length=370, Percent_Identity=28.1081081081081, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50529; Mature: 50529

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNAIVTSPSGSSPAEAQAFLDAHPEIEAFDIVLTDANGVGRGKIVRRHELKSIFEGGRH
CCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCC
MPISILGLDITGEDVHETGLIWTTGDGDLRAWPIPGTLVPLYGTNPPRGQVLMAMYHLDG
CEEEEEEEECCCCCHHHCCCEEECCCCCEEEECCCCEEEEECCCCCCCCCEEEEEEECCC
QPMSSDPRLALARQVDILAAKGLYPAGAFELEFFLLANERDADGKVQPARAVLDGRVSGK
CCCCCCCCEEHHHHHHHHEECCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCC
TEVYSVDHLHGMEPLFSDIYAAAKAQGIPAETVISEYAPGQYELTLNYRKDVMRAADDLV
EEEEEEHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
MLKRLVRAQARRHGVTACFMAKPIEKYAGSGMHFHVSLQDKAGRNVFAEAGGESWSLPLL
HHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHH
QGLGGLIQTMAESMLVFAPHANSWRRFVSQSYAPVAPTWGVNNRSVALRVPAGDAKNRRI
HHHHHHHHHHHHCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
EHRPSGVDANPYLIAATVLAGIVKGLDEGLDPGPETTGNGYEAAVTRTTMPADWRAAIEA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
AKASSFLKGALGEDLHRTFVAIKQSEYLRVARTVSELDYHLYLHEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC
>Mature Secondary Structure
MDNAIVTSPSGSSPAEAQAFLDAHPEIEAFDIVLTDANGVGRGKIVRRHELKSIFEGGRH
CCCCEEECCCCCCCHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCC
MPISILGLDITGEDVHETGLIWTTGDGDLRAWPIPGTLVPLYGTNPPRGQVLMAMYHLDG
CEEEEEEEECCCCCHHHCCCEEECCCCCEEEECCCCEEEEECCCCCCCCCEEEEEEECCC
QPMSSDPRLALARQVDILAAKGLYPAGAFELEFFLLANERDADGKVQPARAVLDGRVSGK
CCCCCCCCEEHHHHHHHHEECCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHCCCCCCC
TEVYSVDHLHGMEPLFSDIYAAAKAQGIPAETVISEYAPGQYELTLNYRKDVMRAADDLV
EEEEEEHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
MLKRLVRAQARRHGVTACFMAKPIEKYAGSGMHFHVSLQDKAGRNVFAEAGGESWSLPLL
HHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHH
QGLGGLIQTMAESMLVFAPHANSWRRFVSQSYAPVAPTWGVNNRSVALRVPAGDAKNRRI
HHHHHHHHHHHHCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCEE
EHRPSGVDANPYLIAATVLAGIVKGLDEGLDPGPETTGNGYEAAVTRTTMPADWRAAIEA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
AKASSFLKGALGEDLHRTFVAIKQSEYLRVARTVSELDYHLYLHEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]