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The map label for this gene is hemL2 [H]

Identifier: 13474293

GI number: 13474293

Start: 4085617

End: 4086999

Strand: Reverse

Name: hemL2 [H]

Synonym: mll5149

Alternate gene names: 13474293

Gene position: 4086999-4085617 (Counterclockwise)

Preceding gene: 13474295

Following gene: 13474292

Centisome position: 58.09

GC content: 67.32

Gene sequence:

>1383_bases
ATGCACAACAGCGACAGTTCAAAGACGCCCATCGGCGGCATCAGCCGTGACCGCATCGCGCAATTGCGCGAGACCGAAGG
CGCGGCCTTCCGCAAGGCGCGGCCGAAATCGCAGGCCAAGGTCGGCAACGGCCTGCCCGGCTTCTTCGGCGGCGTGCCGA
TGCACTGGATGAACGACTGGCCGACGCCGTTTCCGATCCTGGTCGACAGCGCCAAAGGCGCTGCGATCACCGACATCGAT
GGCAACAGGCTCGACGATTTCTGCCTTGGCGACACCGGCTCGATGTTCGGCCACTCGCCACCGCCGGTGGCGCGCGCGAT
CCGCCGCCAGGCCGGGCGCGGCCTGACCTATATGCTGCCTTCGGAAGACGCGCTTGCCATCGGGCCGCTGCTGCAGCAGC
GTTTCGGCCTGCCATTCTGGCAGATCGCGACGACGGCGACCGACGCCAACCGCTTCTCGCTGCGCGTCGCCCGCGCCGTC
ACCGGGCGCGAAAAGATCCTGGTCTTCAACGGCTGCTACCACGGTTCGGTCGACGAGACGATGGTGCGGCTGATCGGCGG
CAAGCCGGTCAACCGGCCGGGACTGGCGGGCGAGTTCCGCGACCTGACCCGTACGGCGAAAGTCATCGAATTCAACGATG
TTGCAGCCCTTGAAGCAGCCCTCAAGGACAGGGATGTGGCCTGCGTCATCGCCGAGCCGGTGCTGACCAATTCCTGCATG
GTGCTGGCCGACCCAGGCTTCCATGACGCGCTGCGCAGACTGACCCGCGAGACCGGCACGCTGCTCCTGATCGACGAGAC
GCACACGATCTCGACAGGTCCGGGCGGCTACACCAGGAAATACGGGCTGGAGCCCGACTTCTTCGTGCTGGGCAAGCCGA
TCGCCGGCGGCGTGCCGGCAAGTGTGTGGGGCATGAGCGACGAGGTCGCCTCGCGCTACGCCGACTACAACAGGACCAAG
GAGCCTGGCTATTCCGGCATGGGCACGACGCTGTCGGCCAACCCGCTGCAGTTCGCGGCGATGCGCGCCACGCTGGAAGA
GGTGATGACGGCGGAGAATTATGACCGCATGGACCATCTGGCGGAGCGGCTCGATGCCGGGCTGACCGGGGTGATCGACC
GCTATCGCCTGCCCTGGCACGTCGCGCGCGTCGGCGCCCGCGTCGAGTTCATCTGCGCACCCGGACCGTTGCGCAATGGC
GCCGAAGCCGAGACCGCGCATGCGCCGGAGCTCGAGGCCGCCATCCACGTCGCGCTGGTCAATCGCGGCGTGCTGATCGC
GCCCTTCCACAACATGATGCTGATCTCGCCGGCGACGTCAGGCGCCCAGGTCAACCGGTTGATCGCCGCCTTCGGCGCGG
TTGCGGCGAAGCTTGCGGCGTGA

Upstream 100 bases:

>100_bases
AAGCGGCGCCGCAGGCGGCTGAGCCAATGCAGGGTAGACGCGCGGCCAATTGCGGTTGCGGACATGGATTGGCTAAGAGG
GACGGGTGAGAGGGGTTTTC

Downstream 100 bases:

>100_bases
GACAAGCGGCCATGGACAATGCCATCGTGACCTCCCCTTCGGGGTCCTCGCCCGCCGAGGCGCAAGCCTTTCTCGACGCC
CATCCCGAGATCGAAGCCTT

Product: aminotransferase

Products: NA

Alternate protein names: GSA 2; Glutamate-1-semialdehyde aminotransferase 2; GSA-AT 2 [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHNSDSSKTPIGGISRDRIAQLRETEGAAFRKARPKSQAKVGNGLPGFFGGVPMHWMNDWPTPFPILVDSAKGAAITDID
GNRLDDFCLGDTGSMFGHSPPPVARAIRRQAGRGLTYMLPSEDALAIGPLLQQRFGLPFWQIATTATDANRFSLRVARAV
TGREKILVFNGCYHGSVDETMVRLIGGKPVNRPGLAGEFRDLTRTAKVIEFNDVAALEAALKDRDVACVIAEPVLTNSCM
VLADPGFHDALRRLTRETGTLLLIDETHTISTGPGGYTRKYGLEPDFFVLGKPIAGGVPASVWGMSDEVASRYADYNRTK
EPGYSGMGTTLSANPLQFAAMRATLEEVMTAENYDRMDHLAERLDAGLTGVIDRYRLPWHVARVGARVEFICAPGPLRNG
AEAETAHAPELEAAIHVALVNRGVLIAPFHNMMLISPATSGAQVNRLIAAFGAVAAKLAA

Sequences:

>Translated_460_residues
MHNSDSSKTPIGGISRDRIAQLRETEGAAFRKARPKSQAKVGNGLPGFFGGVPMHWMNDWPTPFPILVDSAKGAAITDID
GNRLDDFCLGDTGSMFGHSPPPVARAIRRQAGRGLTYMLPSEDALAIGPLLQQRFGLPFWQIATTATDANRFSLRVARAV
TGREKILVFNGCYHGSVDETMVRLIGGKPVNRPGLAGEFRDLTRTAKVIEFNDVAALEAALKDRDVACVIAEPVLTNSCM
VLADPGFHDALRRLTRETGTLLLIDETHTISTGPGGYTRKYGLEPDFFVLGKPIAGGVPASVWGMSDEVASRYADYNRTK
EPGYSGMGTTLSANPLQFAAMRATLEEVMTAENYDRMDHLAERLDAGLTGVIDRYRLPWHVARVGARVEFICAPGPLRNG
AEAETAHAPELEAAIHVALVNRGVLIAPFHNMMLISPATSGAQVNRLIAAFGAVAAKLAA
>Mature_460_residues
MHNSDSSKTPIGGISRDRIAQLRETEGAAFRKARPKSQAKVGNGLPGFFGGVPMHWMNDWPTPFPILVDSAKGAAITDID
GNRLDDFCLGDTGSMFGHSPPPVARAIRRQAGRGLTYMLPSEDALAIGPLLQQRFGLPFWQIATTATDANRFSLRVARAV
TGREKILVFNGCYHGSVDETMVRLIGGKPVNRPGLAGEFRDLTRTAKVIEFNDVAALEAALKDRDVACVIAEPVLTNSCM
VLADPGFHDALRRLTRETGTLLLIDETHTISTGPGGYTRKYGLEPDFFVLGKPIAGGVPASVWGMSDEVASRYADYNRTK
EPGYSGMGTTLSANPLQFAAMRATLEEVMTAENYDRMDHLAERLDAGLTGVIDRYRLPWHVARVGARVEFICAPGPLRNG
AEAETAHAPELEAAIHVALVNRGVLIAPFHNMMLISPATSGAQVNRLIAAFGAVAAKLAA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=412, Percent_Identity=31.7961165048544, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789016, Length=348, Percent_Identity=28.1609195402299, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789759, Length=314, Percent_Identity=25.796178343949, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788044, Length=329, Percent_Identity=26.4437689969605, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787560, Length=327, Percent_Identity=26.9113149847095, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693181, Length=309, Percent_Identity=25.8899676375405, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21357415, Length=314, Percent_Identity=24.8407643312102, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 49531; Mature: 49531

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNSDSSKTPIGGISRDRIAQLRETEGAAFRKARPKSQAKVGNGLPGFFGGVPMHWMNDW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHHHCCCCCCHHHCCCCCHHCCCC
PTPFPILVDSAKGAAITDIDGNRLDDFCLGDTGSMFGHSPPPVARAIRRQAGRGLTYMLP
CCCCCEEEECCCCCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEECC
SEDALAIGPLLQQRFGLPFWQIATTATDANRFSLRVARAVTGREKILVFNGCYHGSVDET
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHEEEEEHHHCCCCEEEEEECCCCCCHHHH
MVRLIGGKPVNRPGLAGEFRDLTRTAKVIEFNDVAALEAALKDRDVACVIAEPVLTNSCM
HHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHCCCCEEEEEECHHHCCCEE
VLADPGFHDALRRLTRETGTLLLIDETHTISTGPGGYTRKYGLEPDFFVLGKPIAGGVPA
EEECCCHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCH
SVWGMSDEVASRYADYNRTKEPGYSGMGTTLSANPLQFAAMRATLEEVMTAENYDRMDHL
HHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AERLDAGLTGVIDRYRLPWHVARVGARVEFICAPGPLRNGAEAETAHAPELEAAIHVALV
HHHHCCCHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEC
NRGVLIAPFHNMMLISPATSGAQVNRLIAAFGAVAAKLAA
CCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHNSDSSKTPIGGISRDRIAQLRETEGAAFRKARPKSQAKVGNGLPGFFGGVPMHWMNDW
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHHHCCCCCCHHHCCCCCHHCCCC
PTPFPILVDSAKGAAITDIDGNRLDDFCLGDTGSMFGHSPPPVARAIRRQAGRGLTYMLP
CCCCCEEEECCCCCEEEECCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCEEEECC
SEDALAIGPLLQQRFGLPFWQIATTATDANRFSLRVARAVTGREKILVFNGCYHGSVDET
CCCCHHHHHHHHHHHCCCCEEEEEECCCCCHHEEEEEHHHCCCCEEEEEECCCCCCHHHH
MVRLIGGKPVNRPGLAGEFRDLTRTAKVIEFNDVAALEAALKDRDVACVIAEPVLTNSCM
HHHHHCCCCCCCCCCCHHHHHHHHHHHEEEECHHHHHHHHHCCCCEEEEEECHHHCCCEE
VLADPGFHDALRRLTRETGTLLLIDETHTISTGPGGYTRKYGLEPDFFVLGKPIAGGVPA
EEECCCHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCH
SVWGMSDEVASRYADYNRTKEPGYSGMGTTLSANPLQFAAMRATLEEVMTAENYDRMDHL
HHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
AERLDAGLTGVIDRYRLPWHVARVGARVEFICAPGPLRNGAEAETAHAPELEAAIHVALV
HHHHCCCHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHEEEEEC
NRGVLIAPFHNMMLISPATSGAQVNRLIAAFGAVAAKLAA
CCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]