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The map label for this gene is yqhT [H]

Identifier: 13473788

GI number: 13473788

Start: 3578898

End: 3580109

Strand: Reverse

Name: yqhT [H]

Synonym: mll4500

Alternate gene names: 13473788

Gene position: 3580109-3578898 (Counterclockwise)

Preceding gene: 13473789

Following gene: 13473787

Centisome position: 50.88

GC content: 64.03

Gene sequence:

>1212_bases
ATGTGGCTGTCGGACACCGAGCCGTCCTTCATCGATGCCGACGCGCTGCGCAAGGGCCGGCTGGCCAAACTGCGGGGCTG
GATGCGCGACGCCGGCTATGGCGGGGTGGTGCTGTTCGACCCGTACAACCAGCGCTATGCCACCGGCTCGCGCAACATGT
TCGGCTATTTCCTGCGCAACTCGACCCGCTATTTCTTCATCCCGACCGAAGGGCCGATCGTGCTGTTCGAGTACCCGCAG
AGCTACCATGTCTCGATGGTGCTCGACACGATCGACGAGGCGCGGCCTTCCAAGCTGGTGTGGTCGTCGGTCTCCGGTCG
CGACGACGAGACCGCCGGCCCCTTCGCCGACGAGATCGCCGAGCTCCTGAAGCAGCACGGCGGCGGCTCGATGAAGCTGG
GTCTCGACCGCTGCAGCCATCTGCTGGCGCTGGCGCTGGAAAAGCGCGGCTGCCAGGTCAAGGATTGCCAGGGCGAAATC
CTGGCGGTGCGCGCGGTGAAGACGCTGGAGGAGGTGAAATGCCTGCAGGTGTCGATGGCCGGCGCCGAGGCCGCCGTCTA
CGCCGTGCGCGAGGCGATCAAGCCCGGCGTCTCCGAAAACGACCTGTTCGCCATCATGTATGGCGAGGTCATCCGCCAGG
GCGGCGAGTTCATCGAGACGCGGCTGCTCACCTCCGGCCAGCGCACCAACCCGTGGTTCAACGAGGCCAGCGGCCGCAAG
ATCCGGCCCGGCGAGCTGCTTGCGCTCGATACCGACACGATCGGCTGCTACGGCTATTATTCCGATTTTTCGCGCACCTT
CCGCTGCGGACCGGGCAAGCCGACCGATTACCAGAAATCGCTCTACCGCATGGCGCATGACCAGGTTCAGCACAACATCT
CGATCGTCAAGCCTGGCATGGCGTTTCGCGAAATCGCCGAGAAGGCCTGGAAGATCCCCGACCGCTTCGTCGACCAGCGC
TATACCTCGGTGATGCACGGCGTCGGCATGCATGGCGAGACACCGTTCATCGCGCATGCCATGGATTACGAGACCTATGG
CCGCGACGGCCATATCGTGCCAGGCATGGTGGTGAGCGTCGAAAGCTATATCGGCGAGAAGGGCGGCCGCGAGGGCGTCA
AGCTGGAGGACGAGATCCTGATCACCGAGACGGGTACGGAGCTGCTGTCGCGCTTTCCCTATGAGGACGAGTTTCTGGAG
AAGCAGGTTTGA

Upstream 100 bases:

>100_bases
GAACCCCATGAGCATCGTCGTATTCGACCCCGACAGCACCGAGGACGTCGACTTCAAGGACCGCATGCGCCACCCGGTGG
CGGCCGATCCGGCGGGCGGA

Downstream 100 bases:

>100_bases
TGGTCTTTGAACCACCCTTGTCAACCAGCGCAGACCCCCACCGCTTCGTCATCCTAGGGCGAAGCAAGGAGCGCAGCGAC
GCGGCGCAGACCCTAGGATC

Product: proline dipeptidase

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MWLSDTEPSFIDADALRKGRLAKLRGWMRDAGYGGVVLFDPYNQRYATGSRNMFGYFLRNSTRYFFIPTEGPIVLFEYPQ
SYHVSMVLDTIDEARPSKLVWSSVSGRDDETAGPFADEIAELLKQHGGGSMKLGLDRCSHLLALALEKRGCQVKDCQGEI
LAVRAVKTLEEVKCLQVSMAGAEAAVYAVREAIKPGVSENDLFAIMYGEVIRQGGEFIETRLLTSGQRTNPWFNEASGRK
IRPGELLALDTDTIGCYGYYSDFSRTFRCGPGKPTDYQKSLYRMAHDQVQHNISIVKPGMAFREIAEKAWKIPDRFVDQR
YTSVMHGVGMHGETPFIAHAMDYETYGRDGHIVPGMVVSVESYIGEKGGREGVKLEDEILITETGTELLSRFPYEDEFLE
KQV

Sequences:

>Translated_403_residues
MWLSDTEPSFIDADALRKGRLAKLRGWMRDAGYGGVVLFDPYNQRYATGSRNMFGYFLRNSTRYFFIPTEGPIVLFEYPQ
SYHVSMVLDTIDEARPSKLVWSSVSGRDDETAGPFADEIAELLKQHGGGSMKLGLDRCSHLLALALEKRGCQVKDCQGEI
LAVRAVKTLEEVKCLQVSMAGAEAAVYAVREAIKPGVSENDLFAIMYGEVIRQGGEFIETRLLTSGQRTNPWFNEASGRK
IRPGELLALDTDTIGCYGYYSDFSRTFRCGPGKPTDYQKSLYRMAHDQVQHNISIVKPGMAFREIAEKAWKIPDRFVDQR
YTSVMHGVGMHGETPFIAHAMDYETYGRDGHIVPGMVVSVESYIGEKGGREGVKLEDEILITETGTELLSRFPYEDEFLE
KQV
>Mature_403_residues
MWLSDTEPSFIDADALRKGRLAKLRGWMRDAGYGGVVLFDPYNQRYATGSRNMFGYFLRNSTRYFFIPTEGPIVLFEYPQ
SYHVSMVLDTIDEARPSKLVWSSVSGRDDETAGPFADEIAELLKQHGGGSMKLGLDRCSHLLALALEKRGCQVKDCQGEI
LAVRAVKTLEEVKCLQVSMAGAEAAVYAVREAIKPGVSENDLFAIMYGEVIRQGGEFIETRLLTSGQRTNPWFNEASGRK
IRPGELLALDTDTIGCYGYYSDFSRTFRCGPGKPTDYQKSLYRMAHDQVQHNISIVKPGMAFREIAEKAWKIPDRFVDQR
YTSVMHGVGMHGETPFIAHAMDYETYGRDGHIVPGMVVSVESYIGEKGGREGVKLEDEILITETGTELLSRFPYEDEFLE
KQV

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

Organism=Escherichia coli, GI1789275, Length=257, Percent_Identity=25.6809338521401, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 45263; Mature: 45263

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLSDTEPSFIDADALRKGRLAKLRGWMRDAGYGGVVLFDPYNQRYATGSRNMFGYFLRN
CCCCCCCCCEECHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEC
STRYFFIPTEGPIVLFEYPQSYHVSMVLDTIDEARPSKLVWSSVSGRDDETAGPFADEIA
CCEEEEEECCCCEEEEECCCCCEEEHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
ELLKQHGGGSMKLGLDRCSHLLALALEKRGCQVKDCQGEILAVRAVKTLEEVKCLQVSMA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHC
GAEAAVYAVREAIKPGVSENDLFAIMYGEVIRQGGEFIETRLLTSGQRTNPWFNEASGRK
CHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCE
IRPGELLALDTDTIGCYGYYSDFSRTFRCGPGKPTDYQKSLYRMAHDQVQHNISIVKPGM
ECCCCEEEEECCCCEEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCH
AFREIAEKAWKIPDRFVDQRYTSVMHGVGMHGETPFIAHAMDYETYGRDGHIVPGMVVSV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHH
ESYIGEKGGREGVKLEDEILITETGTELLSRFPYEDEFLEKQV
HHHHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCCHHHHHCCC
>Mature Secondary Structure
MWLSDTEPSFIDADALRKGRLAKLRGWMRDAGYGGVVLFDPYNQRYATGSRNMFGYFLRN
CCCCCCCCCEECHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEC
STRYFFIPTEGPIVLFEYPQSYHVSMVLDTIDEARPSKLVWSSVSGRDDETAGPFADEIA
CCEEEEEECCCCEEEEECCCCCEEEHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
ELLKQHGGGSMKLGLDRCSHLLALALEKRGCQVKDCQGEILAVRAVKTLEEVKCLQVSMA
HHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCEEECCCCCEEEHHHHHHHHHHHHHHHHHC
GAEAAVYAVREAIKPGVSENDLFAIMYGEVIRQGGEFIETRLLTSGQRTNPWFNEASGRK
CHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCE
IRPGELLALDTDTIGCYGYYSDFSRTFRCGPGKPTDYQKSLYRMAHDQVQHNISIVKPGM
ECCCCEEEEECCCCEEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCEEECCCH
AFREIAEKAWKIPDRFVDQRYTSVMHGVGMHGETPFIAHAMDYETYGRDGHIVPGMVVSV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHH
ESYIGEKGGREGVKLEDEILITETGTELLSRFPYEDEFLEKQV
HHHHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]