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The map label for this gene is irlS [H]

Identifier: 13473758

GI number: 13473758

Start: 3545087

End: 3546463

Strand: Direct

Name: irlS [H]

Synonym: mlr4459

Alternate gene names: 13473758

Gene position: 3545087-3546463 (Clockwise)

Preceding gene: 13473757

Following gene: 13473759

Centisome position: 50.38

GC content: 63.98

Gene sequence:

>1377_bases
ATGACCGGAAGCTGGTCTCGCCTGCTGCGCAGCACGCCGTTCCGGCTCGCCCTGACATTCGGCTTCCTGTTCATGCTGGC
CTTCGTGCTGTCGGGCGCCATCGTCTACCAGATGATGAGCGCCGATCTTGCCGAACGGCTCGACGAGAGCATCAAGGAAA
CCTATTCGGTCGTCGCCGCCACCTATGCCGCGAACGATCTGGAGGACCTTGTCGCCACGATCGAAAGCCATGCGAAGCTC
AGCCCGAAGAAAGAACAGCTGTTTTCGCTGACCGACCCGGCCGGGAACCACCTGGCCGGCAACTTCACCGCCACCGGGCT
TCCCGACGGGTTCTCGGGGTTCGACGCCGTGTTGCCAGGCGTGCCTCCGGATACGGAGTATCGCGCCTTTTCCGGTTCGG
TCGGCGGCAACAATCTGACGGTCGCCTTCAGCCTTTCCGAAACGGAGGAGCTGGAAACAGTGGCGATGATGAGCTTCGGC
TGGGCAACCCTGATCATCACCGGACTGGCGGTCGCCGGCGGCGCCCTGCTTGCCTCGCGCGTCCAGCTCCGTCTCGATGG
CATTGCCGCCACCATGGTCGATGTCTCGCATGGCCGGCTCGATACCCGCATTCCGCTGACCGGCACGGGCGACGACATCG
ACATCGTCTCCAGCCAGGTCAACGCGGCGCTCGATCGCCTGTCGGGCCTGGTGGACGGCATGAAGCAGGTCAGCGCCAAC
ATCGCGCATGATCTGAAGACGCCGCTCAACCGGCTGCAGATGATCCTGGAAGGCGCCGCCGACAAGGCAGCGCGGGATCA
GGACGTCTCCGACGATCTGGCCGACGCGCGTGCGGAGGGCCATCAGATCAACGAGACCTTCGATGCGTTGTTGCGCATCG
CTCAGATCGAGGCCGGTGCCCGCAAGGCGCGGTTCACCGATCTCGATCTCGGCGAGGTGCTGGAAATCATAGCCGAGATC
TACACCGATGTAGCCGAGGACGACGGAAAGTCGCTGGTGTCGACGCAACTGCGTGAGACAGCAGACCCGATTCATGGCGA
TCGGGAACTGTTGACGCAGATGTTCGCCAATCTGGTCGAGAATGCGCTGCGCCATTGCCCGCCCGGGACGACCATCAAAC
TGTCGGTGACGCGCCAGGCCGAGCGTGTCGTTGCCAGCGTCGCCGACAATGGACCCGGCATTCCTCCGGACGAGCGCGAA
CAGGTGTTTCAGCGGCTCTACCGGCTGGACCACAGCCGCTCGACGCCGGGCAACGGCCTTGGCCTCAGCCTGGTGCGGGC
CATTGCCGATTTGCATGGCGCATCCATCGCCCTTGACGATTGTCAGCCTGGCCTCGCCGTGGTGGTGAGCTTTCCGCTGG
TAAGATCGGCGGCGTGA

Upstream 100 bases:

>100_bases
ATATCAGCCGGCTGCGGGCCAAGGTCGACAAGCCGTTCGAGGCCCAGCTCATCCATACCATCCGCAACACCGGCTACAGC
CTGCACGCACCGCTTGCGCC

Downstream 100 bases:

>100_bases
TAGGGCCGAAAGCTGGTGCCAGAAACTTACCAGAGCGCCAATAGCTTACGAAAGGGTATGAGGCCGGCGACCCGCCCGTA
AGCTTGCAGCGGCATAATGA

Product: sensory histidine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTGSWSRLLRSTPFRLALTFGFLFMLAFVLSGAIVYQMMSADLAERLDESIKETYSVVAATYAANDLEDLVATIESHAKL
SPKKEQLFSLTDPAGNHLAGNFTATGLPDGFSGFDAVLPGVPPDTEYRAFSGSVGGNNLTVAFSLSETEELETVAMMSFG
WATLIITGLAVAGGALLASRVQLRLDGIAATMVDVSHGRLDTRIPLTGTGDDIDIVSSQVNAALDRLSGLVDGMKQVSAN
IAHDLKTPLNRLQMILEGAADKAARDQDVSDDLADARAEGHQINETFDALLRIAQIEAGARKARFTDLDLGEVLEIIAEI
YTDVAEDDGKSLVSTQLRETADPIHGDRELLTQMFANLVENALRHCPPGTTIKLSVTRQAERVVASVADNGPGIPPDERE
QVFQRLYRLDHSRSTPGNGLGLSLVRAIADLHGASIALDDCQPGLAVVVSFPLVRSAA

Sequences:

>Translated_458_residues
MTGSWSRLLRSTPFRLALTFGFLFMLAFVLSGAIVYQMMSADLAERLDESIKETYSVVAATYAANDLEDLVATIESHAKL
SPKKEQLFSLTDPAGNHLAGNFTATGLPDGFSGFDAVLPGVPPDTEYRAFSGSVGGNNLTVAFSLSETEELETVAMMSFG
WATLIITGLAVAGGALLASRVQLRLDGIAATMVDVSHGRLDTRIPLTGTGDDIDIVSSQVNAALDRLSGLVDGMKQVSAN
IAHDLKTPLNRLQMILEGAADKAARDQDVSDDLADARAEGHQINETFDALLRIAQIEAGARKARFTDLDLGEVLEIIAEI
YTDVAEDDGKSLVSTQLRETADPIHGDRELLTQMFANLVENALRHCPPGTTIKLSVTRQAERVVASVADNGPGIPPDERE
QVFQRLYRLDHSRSTPGNGLGLSLVRAIADLHGASIALDDCQPGLAVVVSFPLVRSAA
>Mature_457_residues
TGSWSRLLRSTPFRLALTFGFLFMLAFVLSGAIVYQMMSADLAERLDESIKETYSVVAATYAANDLEDLVATIESHAKLS
PKKEQLFSLTDPAGNHLAGNFTATGLPDGFSGFDAVLPGVPPDTEYRAFSGSVGGNNLTVAFSLSETEELETVAMMSFGW
ATLIITGLAVAGGALLASRVQLRLDGIAATMVDVSHGRLDTRIPLTGTGDDIDIVSSQVNAALDRLSGLVDGMKQVSANI
AHDLKTPLNRLQMILEGAADKAARDQDVSDDLADARAEGHQINETFDALLRIAQIEAGARKARFTDLDLGEVLEIIAEIY
TDVAEDDGKSLVSTQLRETADPIHGDRELLTQMFANLVENALRHCPPGTTIKLSVTRQAERVVASVADNGPGIPPDEREQ
VFQRLYRLDHSRSTPGNGLGLSLVRAIADLHGASIALDDCQPGLAVVVSFPLVRSAA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=282, Percent_Identity=30.4964539007092, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786783, Length=368, Percent_Identity=25, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=27.6752767527675, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=330, Percent_Identity=28.4848484848485, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=205, Percent_Identity=27.8048780487805, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790346, Length=270, Percent_Identity=28.5185185185185, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788279, Length=208, Percent_Identity=27.8846153846154, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790551, Length=233, Percent_Identity=30.0429184549356, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790861, Length=304, Percent_Identity=25.6578947368421, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789403, Length=255, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48915; Mature: 48784

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSWSRLLRSTPFRLALTFGFLFMLAFVLSGAIVYQMMSADLAERLDESIKETYSVVAA
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYAANDLEDLVATIESHAKLSPKKEQLFSLTDPAGNHLAGNFTATGLPDGFSGFDAVLPG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHCCC
VPPDTEYRAFSGSVGGNNLTVAFSLSETEELETVAMMSFGWATLIITGLAVAGGALLASR
CCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQLRLDGIAATMVDVSHGRLDTRIPLTGTGDDIDIVSSQVNAALDRLSGLVDGMKQVSAN
HHHHHHHHHHHHHECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHDLKTPLNRLQMILEGAADKAARDQDVSDDLADARAEGHQINETFDALLRIAQIEAGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
RKARFTDLDLGEVLEIIAEIYTDVAEDDGKSLVSTQLRETADPIHGDRELLTQMFANLVE
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
NALRHCPPGTTIKLSVTRQAERVVASVADNGPGIPPDEREQVFQRLYRLDHSRSTPGNGL
HHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
GLSLVRAIADLHGASIALDDCQPGLAVVVSFPLVRSAA
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGSWSRLLRSTPFRLALTFGFLFMLAFVLSGAIVYQMMSADLAERLDESIKETYSVVAA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYAANDLEDLVATIESHAKLSPKKEQLFSLTDPAGNHLAGNFTATGLPDGFSGFDAVLPG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHCCC
VPPDTEYRAFSGSVGGNNLTVAFSLSETEELETVAMMSFGWATLIITGLAVAGGALLASR
CCCCCCCEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQLRLDGIAATMVDVSHGRLDTRIPLTGTGDDIDIVSSQVNAALDRLSGLVDGMKQVSAN
HHHHHHHHHHHHHECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAHDLKTPLNRLQMILEGAADKAARDQDVSDDLADARAEGHQINETFDALLRIAQIEAGA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
RKARFTDLDLGEVLEIIAEIYTDVAEDDGKSLVSTQLRETADPIHGDRELLTQMFANLVE
HHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
NALRHCPPGTTIKLSVTRQAERVVASVADNGPGIPPDEREQVFQRLYRLDHSRSTPGNGL
HHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
GLSLVRAIADLHGASIALDDCQPGLAVVVSFPLVRSAA
HHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]