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The map label for this gene is potB [H]

Identifier: 13473549

GI number: 13473549

Start: 3344372

End: 3345367

Strand: Direct

Name: potB [H]

Synonym: mlr4191

Alternate gene names: 13473549

Gene position: 3344372-3345367 (Clockwise)

Preceding gene: 13473548

Following gene: 13473550

Centisome position: 47.53

GC content: 65.36

Gene sequence:

>996_bases
ATGTCGGCGAAGCCCAGCGCGACTATTCCAAGATCGTCAGCGAGATCAAGGCCGCGCAGTAGGCGGCACGGATATCACGG
CCGGCAGTGCTTCCGCTGCCGGCCTGAGCGGTACCTTAAATTGTCCCAGACCCGACTTCGCTCCTCCCTGCTGATGGCCC
CGGCGCTGGTCTGGTTGACCGCGCTGATGGTGGTGCCGTGCGCGCTGATGCTGGCGCTCGCCTTCTTTCGACGCGGCATC
TATGGCGGTATCGACTACACCTTCACGCTGGAGAATTTCGGGCTGGTCTTCGACCCGCTCTATGCCAGCATTTTCCTGAA
GTCAGCGCGCATCGCCGGCACTGCCACGCTGATCGCGGTGGTGATCGGCTATGCCGCCGCCTACGCCATCTCCGCTGCCC
CGCGCCGCTGGCAGCCTGTGTTCCTGTTCTTTGCCGTGCTGCCGTTCTGGTCGAACTATTTGATCCGCACCTATGCCTGG
ATCGTGCTGCTCAACCGCGAAGGCCTGATCACGCAGCTCCTGCGCTGGTCGGGCTATACCGGCGAACCGCCATCGATGCT
CTACACCGAAGGCGCGGTCATCGCCGGCCTCGTCTACAATTATCTGCCCTTCGTCATCCTCGCCTGCTACGCGCCGCTGT
CGCGGCTCAACCCGGAACTGGCCGAGGCCTCACGTGATCTCGGCGCCTCGGCCGCCACCACCTTCCGCCGTGTCATCCTG
CCGCTGACCGTGCCCGGCATCGCCGCCGGCGCTGTCTTCGTCTTCGTGCTGTCGATCGGCAATTTCGTCACTCCGGCGCT
GCTCGGCGGCGGCCGCTTCCAGATGATCGGCAATCTCGTCTACGACCAGTTCCTCACCGCCAATGACTGGCCCTTTGGCG
CAGCCCTCGCCATGGCGCTGATCGCCATCATGCTGCTGGTGCTGATGGCGCAGGCGCTCGCGGCCGACCGCGCCTCGGGG
CGTGCGGCGCAGGCCGTGGGAGGCACTGATGGCTGA

Upstream 100 bases:

>100_bases
AACAAGCCGGCGATGGAAAGCCTGCCGGCCGATTTCCTGGCGAAATTCCCCAACATGGCGATGCCGGTGGCCGATCTCGT
CAAGTTTGAGCAGCTGCGCG

Downstream 100 bases:

>100_bases
GCGCTCCCCTGCGACGCGGCGCACGCTGTGGCTCGTGCTGAGCCTGGTCTTCGCCTTCCTCTACATCCCGATCGCCGTGC
TGGTGGCGCTCTCCTTCAAC

Product: spermidine/putrescine ABC transporter, permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSAKPSATIPRSSARSRPRSRRHGYHGRQCFRCRPERYLKLSQTRLRSSLLMAPALVWLTALMVVPCALMLALAFFRRGI
YGGIDYTFTLENFGLVFDPLYASIFLKSARIAGTATLIAVVIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAW
IVLLNREGLITQLLRWSGYTGEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDLGASAATTFRRVIL
PLTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQFLTANDWPFGAALAMALIAIMLLVLMAQALAADRASG
RAAQAVGGTDG

Sequences:

>Translated_331_residues
MSAKPSATIPRSSARSRPRSRRHGYHGRQCFRCRPERYLKLSQTRLRSSLLMAPALVWLTALMVVPCALMLALAFFRRGI
YGGIDYTFTLENFGLVFDPLYASIFLKSARIAGTATLIAVVIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAW
IVLLNREGLITQLLRWSGYTGEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDLGASAATTFRRVIL
PLTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQFLTANDWPFGAALAMALIAIMLLVLMAQALAADRASG
RAAQAVGGTDG
>Mature_330_residues
SAKPSATIPRSSARSRPRSRRHGYHGRQCFRCRPERYLKLSQTRLRSSLLMAPALVWLTALMVVPCALMLALAFFRRGIY
GGIDYTFTLENFGLVFDPLYASIFLKSARIAGTATLIAVVIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAWI
VLLNREGLITQLLRWSGYTGEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDLGASAATTFRRVILP
LTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQFLTANDWPFGAALAMALIAIMLLVLMAQALAADRASGR
AAQAVGGTDG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=38.7351778656126, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787080, Length=241, Percent_Identity=36.5145228215768, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787713, Length=256, Percent_Identity=32.8125, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787368, Length=123, Percent_Identity=30.0813008130081, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36092; Mature: 35961

Theoretical pI: Translated: 10.40; Mature: 10.40

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKPSATIPRSSARSRPRSRRHGYHGRQCFRCRPERYLKLSQTRLRSSLLMAPALVWLT
CCCCCCCCCCCCHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMVVPCALMLALAFFRRGIYGGIDYTFTLENFGLVFDPLYASIFLKSARIAGTATLIAV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAWIVLLNREGLITQLLRWSGYT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCC
GEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDLGASAATTFRRVIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
PLTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQFLTANDWPFGAALAMAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IAIMLLVLMAQALAADRASGRAAQAVGGTDG
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
SAKPSATIPRSSARSRPRSRRHGYHGRQCFRCRPERYLKLSQTRLRSSLLMAPALVWLT
CCCCCCCCCCCHHHCCCHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMVVPCALMLALAFFRRGIYGGIDYTFTLENFGLVFDPLYASIFLKSARIAGTATLIAV
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAWIVLLNREGLITQLLRWSGYT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHCCCC
GEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDLGASAATTFRRVIL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHH
PLTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQFLTANDWPFGAALAMAL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IAIMLLVLMAQALAADRASGRAAQAVGGTDG
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]