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The map label for this gene is potD-B [H]

Identifier: 13473548

GI number: 13473548

Start: 3343369

End: 3344433

Strand: Direct

Name: potD-B [H]

Synonym: mlr4190

Alternate gene names: 13473548

Gene position: 3343369-3344433 (Clockwise)

Preceding gene: 13473546

Following gene: 13473549

Centisome position: 47.52

GC content: 62.44

Gene sequence:

>1065_bases
ATGATCGCTACCAGCCCGCGGCGCCGTGCGCTCAAGGTGTCTTCAATCGCTCTCGGCCTTGCCCTCGCGCTGTCGGCGCC
GGCAATGGCCGCCGATCTCGTCATCTCCAACTGGGACGGCTACATGGCGCCCGACGCCATGGCGGCCTTCAAGACCGCGA
CCGGCGTATCCGGGGAAGTGGTGGTGCACGCCACCAATGAGGAGATCATGGGCAAGCTGATTGCCGCCGGTGGCAAGGGC
TATGACGTCGTCTTCGTCTCTTCGCCCTTCGCCGAGGTGCTGAACAAGCTCGGCCTGACCGAGCCGATGGACCACGCCAA
GATCCCCAATCTCGCCAACCTCTATCCGGAAGCGACCAAGCTGCCGCACGATGTCGGCAACTCGTTCTCCGTGCCCTATA
CCTGGGGCACGACCGGCCTCTGCTACCGCTCGGACCTGGTCAAGACGGCGCCGACGAGCTGGAGCGACCTGCTCGCGCCT
TCAGACGCACTGAAGGGCAAGACCACCATGCTGGCGACCGACCGCTGGCTGCTGGCAGCCGGGCAGCTCGATAAGGGCTT
CTCCGTCAACGAGACCGATCCGGCCAAGATGGCCGAGGTCAAGGACCTCTTGATCTCGGCCAAGAAGACCCTGCTCGCCT
ATGACGACACCACCTTCTATTCCAAGCTGGTTTCGGGCGAGGCGCTGATGGTGCAGGCCTGGGATGGCTGGTGCAATTAC
GGCATCGCCGAGAAGCCGGAGATCAAATACGTCATTCCGAAGGAAGGTTCGGACCTCTGGGTCGACACGATGGTGGTGAT
GAAGGCCTCCGAGCACAAGGACGACGCCTTCAAGTTCATCAACTTCATGCTCGACGCCAAGAACCACGCCTGGGCGGCGC
AGAACATCGACTACAAGGTGCCGAACAAGCCGGCGATGGAAAGCCTGCCGGCCGATTTCCTGGCGAAATTCCCCAACATG
GCGATGCCGGTGGCCGATCTCGTCAAGTTTGAGCAGCTGCGCGATGTCGGCGAAGCCCAGCGCGACTATTCCAAGATCGT
CAGCGAGATCAAGGCCGCGCAGTAG

Upstream 100 bases:

>100_bases
CGGCGACAAAAAAGACCGAGGGAACAGGATCGAAGGCAGCGGCATGGGTCGCGCCCGGTCCAGCACAGAATTCGACGATC
AACCGGAATGGGAGTCTGCC

Downstream 100 bases:

>100_bases
GCGGCACGGATATCACGGCCGGCAGTGCTTCCGCTGCCGGCCTGAGCGGTACCTTAAATTGTCCCAGACCCGACTTCGCT
CCTCCCTGCTGATGGCCCCG

Product: spermidine/putrescine ABC transporter, periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MIATSPRRRALKVSSIALGLALALSAPAMAADLVISNWDGYMAPDAMAAFKTATGVSGEVVVHATNEEIMGKLIAAGGKG
YDVVFVSSPFAEVLNKLGLTEPMDHAKIPNLANLYPEATKLPHDVGNSFSVPYTWGTTGLCYRSDLVKTAPTSWSDLLAP
SDALKGKTTMLATDRWLLAAGQLDKGFSVNETDPAKMAEVKDLLISAKKTLLAYDDTTFYSKLVSGEALMVQAWDGWCNY
GIAEKPEIKYVIPKEGSDLWVDTMVVMKASEHKDDAFKFINFMLDAKNHAWAAQNIDYKVPNKPAMESLPADFLAKFPNM
AMPVADLVKFEQLRDVGEAQRDYSKIVSEIKAAQ

Sequences:

>Translated_354_residues
MIATSPRRRALKVSSIALGLALALSAPAMAADLVISNWDGYMAPDAMAAFKTATGVSGEVVVHATNEEIMGKLIAAGGKG
YDVVFVSSPFAEVLNKLGLTEPMDHAKIPNLANLYPEATKLPHDVGNSFSVPYTWGTTGLCYRSDLVKTAPTSWSDLLAP
SDALKGKTTMLATDRWLLAAGQLDKGFSVNETDPAKMAEVKDLLISAKKTLLAYDDTTFYSKLVSGEALMVQAWDGWCNY
GIAEKPEIKYVIPKEGSDLWVDTMVVMKASEHKDDAFKFINFMLDAKNHAWAAQNIDYKVPNKPAMESLPADFLAKFPNM
AMPVADLVKFEQLRDVGEAQRDYSKIVSEIKAAQ
>Mature_354_residues
MIATSPRRRALKVSSIALGLALALSAPAMAADLVISNWDGYMAPDAMAAFKTATGVSGEVVVHATNEEIMGKLIAAGGKG
YDVVFVSSPFAEVLNKLGLTEPMDHAKIPNLANLYPEATKLPHDVGNSFSVPYTWGTTGLCYRSDLVKTAPTSWSDLLAP
SDALKGKTTMLATDRWLLAAGQLDKGFSVNETDPAKMAEVKDLLISAKKTLLAYDDTTFYSKLVSGEALMVQAWDGWCNY
GIAEKPEIKYVIPKEGSDLWVDTMVVMKASEHKDDAFKFINFMLDAKNHAWAAQNIDYKVPNKPAMESLPADFLAKFPNM
AMPVADLVKFEQLRDVGEAQRDYSKIVSEIKAAQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=326, Percent_Identity=27.9141104294479, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787078, Length=314, Percent_Identity=30.5732484076433, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787711, Length=282, Percent_Identity=26.9503546099291, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38510; Mature: 38510

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATSPRRRALKVSSIALGLALALSAPAMAADLVISNWDGYMAPDAMAAFKTATGVSGEV
CCCCCCCHHHEEHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCEE
VVHATNEEIMGKLIAAGGKGYDVVFVSSPFAEVLNKLGLTEPMDHAKIPNLANLYPEATK
EEEECCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCHHCCCCCHHHCCCCHHC
LPHDVGNSFSVPYTWGTTGLCYRSDLVKTAPTSWSDLLAPSDALKGKTTMLATDRWLLAA
CCHHCCCCCCCCEECCCCCCEEHHHHHHCCCCCHHHHCCCCHHHCCCEEEEECCCEEEEE
GQLDKGFSVNETDPAKMAEVKDLLISAKKTLLAYDDTTFYSKLVSGEALMVQAWDGWCNY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCEEEEEECCCHHCC
GIAEKPEIKYVIPKEGSDLWVDTMVVMKASEHKDDAFKFINFMLDAKNHAWAAQNIDYKV
CCCCCCCEEEECCCCCCCEEHHHEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCEEC
PNKPAMESLPADFLAKFPNMAMPVADLVKFEQLRDVGEAQRDYSKIVSEIKAAQ
CCCCHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIATSPRRRALKVSSIALGLALALSAPAMAADLVISNWDGYMAPDAMAAFKTATGVSGEV
CCCCCCCHHHEEHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCEE
VVHATNEEIMGKLIAAGGKGYDVVFVSSPFAEVLNKLGLTEPMDHAKIPNLANLYPEATK
EEEECCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCHHCCCCCHHHCCCCHHC
LPHDVGNSFSVPYTWGTTGLCYRSDLVKTAPTSWSDLLAPSDALKGKTTMLATDRWLLAA
CCHHCCCCCCCCEECCCCCCEEHHHHHHCCCCCHHHHCCCCHHHCCCEEEEECCCEEEEE
GQLDKGFSVNETDPAKMAEVKDLLISAKKTLLAYDDTTFYSKLVSGEALMVQAWDGWCNY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHCCCEEEEEECCCHHCC
GIAEKPEIKYVIPKEGSDLWVDTMVVMKASEHKDDAFKFINFMLDAKNHAWAAQNIDYKV
CCCCCCCEEEECCCCCCCEEHHHEEEEECCCCCHHHHHHHHHHHCCCCCCEEECCCCEEC
PNKPAMESLPADFLAKFPNMAMPVADLVKFEQLRDVGEAQRDYSKIVSEIKAAQ
CCCCHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]