BacMap

Definition	Mesorhizobium loti MAFF303099 chromosome, complete genome.
Accession	NC_002678
Length	7,036,071

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The map label for this gene is yusZ [H]

Identifier: 13472244

GI number: 13472244

Start: 2009518

End: 2010348

Strand: Direct

Name: yusZ [H]

Synonym: mlr2473

Alternate gene names: 13472244

Gene position: 2009518-2010348 (Clockwise)

Preceding gene: 13472242

Following gene: 13472246

Centisome position: 28.56

GC content: 63.9

Gene sequence:

>831_bases
ATGACCAGGACATGGTTCATCACCGGTACTTCATCGGGCTTCGGACGGCTTTTGACGGACAGGCTTCTGGCCCGTGGCGA
CCGGGTCGCCGCGACAGTGCGGAGACCGCAGGCTCTCGCGGACCTGAAGGCGCTGCATGGCGATCGCCTCTGGATCGCTT
CCCTTGAAATGACCGACAGCGATGCCATCCGGGCAACGGTCGACAAGGCCTTTGGCGAGTTGGGGCGCATCGATGTCATC
GTCAGCAACGCCGGCTATGGCCTGTTCGGCGCCGCCGAGGAGCTGGAAGACAGCCAGATCCGCGACCTGATCGACACAAA
TCTCATCGGCTCCATACAGTTGATCCGGGTGGCACTGCCGCATCTGCGCAAACAGCGCGGCGGCCGCATCCTGCAAGTCT
CCTCGGAGGGCGGGCAGATGGCCTATCCCGGCTTCAGCCTCTATCACGCCAGCAAATGGGGCATTGAAGGCTTTGTCGAA
TCCGTTCGCCAGGAGGTCGCGTCGTTCGGCATCGGGCTGACGCTGGTCGAGCCAGGCCCGACACGGACGAATTTCGGCGC
CAGCCTGGTCACTTCGGGGTCCACGGATGCCTATGCGAACACGCCGGTTGGCGACCTGCACCGGGCTTTTTCCGAGGGTT
CGTTCGACATCAAGGGCGATCCCGGCAAGATGGTCGAGGCGATGATCGCCTCGGTGGAACAGGATCCCGCGCCTCGACGA
TTGACCTTCGGCGCCGCAGCCTACGCTTCGATCCGCAGCGCGCTCCAGGGCAGGCTCGCGGAACTGGAGAACCGGAAGGA
GGCGGCACTCGCCATGGATATCGACCCCTGA

Upstream 100 bases:

>100_bases
TCATGGAAAATCTTCATCAAGCCATGTGAAATTGAACGGATAGTATCATGAGTTGGATGGCGTAGTCTTGGCCGAAGTCA
GCAATGAAAGGACATTTCCC

Downstream 100 bases:

>100_bases
CGGCTTGCCGGTCGAACGGCGTCGGCCGCAGCCCTCACCCGCGCCAGCGCAACAGGCGCATGGCGTTGGCGGTGACCAGC
ACGGTGGCGCCGGTGTCGGC

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: ORFA [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTRTWFITGTSSGFGRLLTDRLLARGDRVAATVRRPQALADLKALHGDRLWIASLEMTDSDAIRATVDKAFGELGRIDVI
VSNAGYGLFGAAEELEDSQIRDLIDTNLIGSIQLIRVALPHLRKQRGGRILQVSSEGGQMAYPGFSLYHASKWGIEGFVE
SVRQEVASFGIGLTLVEPGPTRTNFGASLVTSGSTDAYANTPVGDLHRAFSEGSFDIKGDPGKMVEAMIASVEQDPAPRR
LTFGAAAYASIRSALQGRLAELENRKEAALAMDIDP

Sequences:

>Translated_276_residues
MTRTWFITGTSSGFGRLLTDRLLARGDRVAATVRRPQALADLKALHGDRLWIASLEMTDSDAIRATVDKAFGELGRIDVI
VSNAGYGLFGAAEELEDSQIRDLIDTNLIGSIQLIRVALPHLRKQRGGRILQVSSEGGQMAYPGFSLYHASKWGIEGFVE
SVRQEVASFGIGLTLVEPGPTRTNFGASLVTSGSTDAYANTPVGDLHRAFSEGSFDIKGDPGKMVEAMIASVEQDPAPRR
LTFGAAAYASIRSALQGRLAELENRKEAALAMDIDP
>Mature_275_residues
TRTWFITGTSSGFGRLLTDRLLARGDRVAATVRRPQALADLKALHGDRLWIASLEMTDSDAIRATVDKAFGELGRIDVIV
SNAGYGLFGAAEELEDSQIRDLIDTNLIGSIQLIRVALPHLRKQRGGRILQVSSEGGQMAYPGFSLYHASKWGIEGFVES
VRQEVASFGIGLTLVEPGPTRTNFGASLVTSGSTDAYANTPVGDLHRAFSEGSFDIKGDPGKMVEAMIASVEQDPAPRRL
TFGAAAYASIRSALQGRLAELENRKEAALAMDIDP

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7657478, Length=252, Percent_Identity=30.1587301587302, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI223718074, Length=228, Percent_Identity=32.8947368421053, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI22507405, Length=182, Percent_Identity=37.3626373626374, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI150247226, Length=182, Percent_Identity=36.2637362637363, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=29.2307692307692, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI19743808, Length=182, Percent_Identity=37.3626373626374, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI40548397, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI214010158, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI214010156, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI22758144, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI4503817, Length=185, Percent_Identity=29.1891891891892, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI7706318, Length=188, Percent_Identity=29.7872340425532, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI50726952, Length=182, Percent_Identity=36.8131868131868, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI44680136, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI44680133, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI17738292, Length=205, Percent_Identity=30.2439024390244, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI119392083, Length=189, Percent_Identity=30.1587301587302, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI40254992, Length=190, Percent_Identity=29.4736842105263, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI32455239, Length=188, Percent_Identity=30.3191489361702, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI5031765, Length=188, Percent_Identity=30.3191489361702, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI31542939, Length=191, Percent_Identity=26.7015706806283, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787335, Length=207, Percent_Identity=32.3671497584541, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787820, Length=177, Percent_Identity=32.2033898305085, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786701, Length=205, Percent_Identity=30.2439024390244, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI87082100, Length=191, Percent_Identity=30.3664921465969, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786812, Length=138, Percent_Identity=28.2608695652174, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367365, Length=187, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534660, Length=182, Percent_Identity=29.1208791208791, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=187, Percent_Identity=29.4117647058824, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=187, Percent_Identity=28.3422459893048, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17507613, Length=207, Percent_Identity=29.951690821256, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32563809, Length=207, Percent_Identity=29.951690821256, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557780, Length=188, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17567345, Length=192, Percent_Identity=27.0833333333333, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=194, Percent_Identity=25.2577319587629, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17570069, Length=188, Percent_Identity=27.6595744680851, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=188, Percent_Identity=25.531914893617, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=206, Percent_Identity=28.1553398058252, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=194, Percent_Identity=27.319587628866, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=205, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI193210355, Length=184, Percent_Identity=29.3478260869565, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=193, Percent_Identity=30.0518134715026, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322226, Length=168, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322861, Length=112, Percent_Identity=30.3571428571429, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358495, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI23397609, Length=229, Percent_Identity=32.3144104803493, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI116007236, Length=193, Percent_Identity=28.4974093264249, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644339, Length=259, Percent_Identity=28.957528957529, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI28571526, Length=187, Percent_Identity=29.9465240641711, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355319, Length=215, Percent_Identity=26.046511627907, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007238, Length=175, Percent_Identity=29.1428571428571, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580925, Length=191, Percent_Identity=26.7015706806283, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355631, Length=168, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356301, Length=189, Percent_Identity=24.3386243386243, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21357041, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571387, Length=193, Percent_Identity=31.0880829015544, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24643142, Length=185, Percent_Identity=25.4054054054054, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581726, Length=143, Percent_Identity=28.6713286713287, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281364494, Length=193, Percent_Identity=27.4611398963731, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 29695; Mature: 29564

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTWFITGTSSGFGRLLTDRLLARGDRVAATVRRPQALADLKALHGDRLWIASLEMTDS
CCEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCEEEEEEEEECCC
DAIRATVDKAFGELGRIDVIVSNAGYGLFGAAEELEDSQIRDLIDTNLIGSIQLIRVALP
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HLRKQRGGRILQVSSEGGQMAYPGFSLYHASKWGIEGFVESVRQEVASFGIGLTLVEPGP
HHHHHHCCEEEEEECCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
TRTNFGASLVTSGSTDAYANTPVGDLHRAFSEGSFDIKGDPGKMVEAMIASVEQDPAPRR
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCCE
LTFGAAAYASIRSALQGRLAELENRKEAALAMDIDP
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
>Mature Secondary Structure 
TRTWFITGTSSGFGRLLTDRLLARGDRVAATVRRPQALADLKALHGDRLWIASLEMTDS
CEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCCEEEEEEEEECCC
DAIRATVDKAFGELGRIDVIVSNAGYGLFGAAEELEDSQIRDLIDTNLIGSIQLIRVALP
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
HLRKQRGGRILQVSSEGGQMAYPGFSLYHASKWGIEGFVESVRQEVASFGIGLTLVEPGP
HHHHHHCCEEEEEECCCCEEECCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
TRTNFGASLVTSGSTDAYANTPVGDLHRAFSEGSFDIKGDPGKMVEAMIASVEQDPAPRR
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCCE
LTFGAAAYASIRSALQGRLAELENRKEAALAMDIDP
EEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 8396117 [H]