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The map label for this gene is hyi [C]

Identifier: 13471116

GI number: 13471116

Start: 826759

End: 827616

Strand: Reverse

Name: hyi [C]

Synonym: mll1001

Alternate gene names: 13471116

Gene position: 827616-826759 (Counterclockwise)

Preceding gene: 13471117

Following gene: 13471115

Centisome position: 11.76

GC content: 64.8

Gene sequence:

>858_bases
ATGTCGGTGCGGCGCAATCGCGCCGCACCACTCATCGTCATACCCACAATTCGAGAACCGCCAATGAGTGTATCCACGAC
CGCTCCCTTTCCCCTCGCAGCCTGCGCCGAGATGCTGTGGCGCGACAAGCCGATCGAATGGCGTGCCTCGCGCCTCAGGG
AGATGGGCTTCGGCGTCGGCCTCTGGAATTGGCCGGAGCACGATCTCGCCAAGCTCGAGGCGACCGGAGCGACCTTCACG
ATCATGAACGGCTACCTTGCGGGCCGTCTGACCGATGATGAAGGTGCGGCCGAACTGATCAGAACGGCCCGCGAGACCGC
ACAGGTTGGCAAGCGCCTCGGGGTGCAGCGCCTCAACCTGCACGGCACGGGGCTTGGGGACAGGGGCCTCCCAGTTCAGC
CCATCGAAGTCGTCACCGGTGCAATGTGGCTGAAGGCGCGTGACACTCTCTGCCGCGTCGTCGACCTAGCCGAGCAGGAA
GGTGTCACCTTCACGATTGAAAATCTCAACCTGCCGGTCGACCACCCAGGCGTGCCCTTTGGCCGTGCCGAGGACACGCT
CGCCCTGGTGTCGAGCATCGGCCATGCGCGACTTCGCCTGAACCTCGATCTCTACCACGCCCAGATCGGCGAGGGGAATT
TGATCGAACTCTGCCGCAAGTGCCTGCCCTGGATCGGCGAGATCCAGGTGGCGGACGTTCCGGGCCGCTGCGAACCCGGC
ACCGGCGAGATTAACTGGCGTGGTGTCGCCAAAGCCCTGAAGGCAATGGATTATTCCGGCCCTGTCGGCATGGAAGCCTG
GGCCGCCGGCGATCCCGACGCCGCGCTCGAAGCCTTCCGCGAGGCCTTCACGGTCTGA

Upstream 100 bases:

>100_bases
GATCGGCATCGACGAGCTCCAGAACCTGATGGCAGGCGGCAAGGAGCTGCAGTCGCTCTCAGAGGAACTCGGCGGAACGA
TCTGATCGAGAGCTTTTCAC

Downstream 100 bases:

>100_bases
CTTACCTCTCCAACCCATTTTTAGGATTGCTCGAATGCCCAAGAATAAGCCTGTCAGTGTCGGACTCATCGGCGCCGGTC
GCATCGGTTCTTTCCACGGT

Product: hypothetical protein

Products: 2-Hydroxy-3-oxopropanoate [C]

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSVRRNRAAPLIVIPTIREPPMSVSTTAPFPLAACAEMLWRDKPIEWRASRLREMGFGVGLWNWPEHDLAKLEATGATFT
IMNGYLAGRLTDDEGAAELIRTARETAQVGKRLGVQRLNLHGTGLGDRGLPVQPIEVVTGAMWLKARDTLCRVVDLAEQE
GVTFTIENLNLPVDHPGVPFGRAEDTLALVSSIGHARLRLNLDLYHAQIGEGNLIELCRKCLPWIGEIQVADVPGRCEPG
TGEINWRGVAKALKAMDYSGPVGMEAWAAGDPDAALEAFREAFTV

Sequences:

>Translated_285_residues
MSVRRNRAAPLIVIPTIREPPMSVSTTAPFPLAACAEMLWRDKPIEWRASRLREMGFGVGLWNWPEHDLAKLEATGATFT
IMNGYLAGRLTDDEGAAELIRTARETAQVGKRLGVQRLNLHGTGLGDRGLPVQPIEVVTGAMWLKARDTLCRVVDLAEQE
GVTFTIENLNLPVDHPGVPFGRAEDTLALVSSIGHARLRLNLDLYHAQIGEGNLIELCRKCLPWIGEIQVADVPGRCEPG
TGEINWRGVAKALKAMDYSGPVGMEAWAAGDPDAALEAFREAFTV
>Mature_284_residues
SVRRNRAAPLIVIPTIREPPMSVSTTAPFPLAACAEMLWRDKPIEWRASRLREMGFGVGLWNWPEHDLAKLEATGATFTI
MNGYLAGRLTDDEGAAELIRTARETAQVGKRLGVQRLNLHGTGLGDRGLPVQPIEVVTGAMWLKARDTLCRVVDLAEQEG
VTFTIENLNLPVDHPGVPFGRAEDTLALVSSIGHARLRLNLDLYHAQIGEGNLIELCRKCLPWIGEIQVADVPGRCEPGT
GEINWRGVAKALKAMDYSGPVGMEAWAAGDPDAALEAFREAFTV

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=125, Percent_Identity=32.8, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI300068964, Length=117, Percent_Identity=32.4786324786325, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1786718, Length=118, Percent_Identity=34.7457627118644, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22 [C]

Molecular weight: Translated: 31110; Mature: 30979

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRNRAAPLIVIPTIREPPMSVSTTAPFPLAACAEMLWRDKPIEWRASRLREMGFGVG
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
LWNWPEHDLAKLEATGATFTIMNGYLAGRLTDDEGAAELIRTARETAQVGKRLGVQRLNL
CCCCCCHHHHHEECCCCEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEE
HGTGLGDRGLPVQPIEVVTGAMWLKARDTLCRVVDLAEQEGVTFTIENLNLPVDHPGVPF
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GRAEDTLALVSSIGHARLRLNLDLYHAQIGEGNLIELCRKCLPWIGEIQVADVPGRCEPG
CCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCC
TGEINWRGVAKALKAMDYSGPVGMEAWAAGDPDAALEAFREAFTV
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SVRRNRAAPLIVIPTIREPPMSVSTTAPFPLAACAEMLWRDKPIEWRASRLREMGFGVG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
LWNWPEHDLAKLEATGATFTIMNGYLAGRLTDDEGAAELIRTARETAQVGKRLGVQRLNL
CCCCCCHHHHHEECCCCEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEE
HGTGLGDRGLPVQPIEVVTGAMWLKARDTLCRVVDLAEQEGVTFTIENLNLPVDHPGVPF
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
GRAEDTLALVSSIGHARLRLNLDLYHAQIGEGNLIELCRKCLPWIGEIQVADVPGRCEPG
CCCHHHHHHHHHCCCEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCC
TGEINWRGVAKALKAMDYSGPVGMEAWAAGDPDAALEAFREAFTV
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: Hydroxypyruvate [C]

Specific reaction: Hydroxypyruvate <==> 2-Hydroxy-3-oxopropanoate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]