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The map label for this gene is gfa [H]

Identifier: 13471014

GI number: 13471014

Start: 702658

End: 703332

Strand: Direct

Name: gfa [H]

Synonym: mlr0874

Alternate gene names: 13471014

Gene position: 702658-703332 (Clockwise)

Preceding gene: 13471013

Following gene: 13471015

Centisome position: 9.99

GC content: 62.81

Gene sequence:

>675_bases
ATGAGGGCAAGTCGATCCGTAGCGTCGTGGTTTACTGAAGCAAGCCACACGCCGCCGACCGACCGGCCTGCGACCTCTGT
GGTTCAAAACTGGGAGGAGAAAAGAATGGCGGAAAAACTGCATCCGAGAATCGACAATGGCCTGCCCAAGGAGAGCGCGA
GCTTTGCCGGCGGCACGCTGGTCTGCGCCTGCACCAGCAAGCCGGTCAAGGTGAAGGTCAAGGGGCAGATCGCCCACAAC
CATGCCTGCGGCTGCACCAAATGCTGGAAGCCGGAAGGTGCTATATTCTCGGTGGTTGCCGTTGCCGGCACCGGCGACGT
CACTGTCACCGAGAACGGCGACAAGCTGAAAGTGGTCGACGCTAGCGCGCTGATCCAGCGCCATGCCTGCACCGGCTGCG
GCGTCCACATGCACGGACCGGTCGAGCGCGACCATGCGTTCAAAGGCCTGACCTTCATCCACCCCGAGCGCTTCGTCGAG
GATGGTTGGTCGCCACCGGGCTTCACCGCCTTCGTCTCGTCGATCATCGAATCCGGCGTCGATCCAAAGCGGATGGACGG
CATCCGGGCGCAGCTAAGGACGATCGGGCTGGAGCCCTATGACTGCCTCAACCCCGGCCTGATGGACTATATCGCCACCT
GGACGGCGAAGAAGTCCGGCGCCTTGCCGGCCTGA

Upstream 100 bases:

>100_bases
CGGATCGTCGACTGGTACATGGACGGCAAGATCCAGATCGATCCGATGATCACCCATTTGCTGAAGCTGGAAGACATCAA
CAAGGGGTTCGACCTCATGC

Downstream 100 bases:

>100_bases
ACCCTGTAAGATTTCAGGCAGGGGCCGGACATGTTTCCGGCCCCTGTCCGTTTCGGAGAGATTATGCCCGCCCCCACCAT
TTACGTCGATGCCGACGCCT

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRASRSVASWFTEASHTPPTDRPATSVVQNWEEKRMAEKLHPRIDNGLPKESASFAGGTLVCACTSKPVKVKVKGQIAHN
HACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVE
DGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYDCLNPGLMDYIATWTAKKSGALPA

Sequences:

>Translated_224_residues
MRASRSVASWFTEASHTPPTDRPATSVVQNWEEKRMAEKLHPRIDNGLPKESASFAGGTLVCACTSKPVKVKVKGQIAHN
HACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVE
DGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYDCLNPGLMDYIATWTAKKSGALPA
>Mature_224_residues
MRASRSVASWFTEASHTPPTDRPATSVVQNWEEKRMAEKLHPRIDNGLPKESASFAGGTLVCACTSKPVKVKVKGQIAHN
HACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVDASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVE
DGWSPPGFTAFVSSIIESGVDPKRMDGIRAQLRTIGLEPYDCLNPGLMDYIATWTAKKSGALPA

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 24124; Mature: 24124

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASRSVASWFTEASHTPPTDRPATSVVQNWEEKRMAEKLHPRIDNGLPKESASFAGGTL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEE
VCACTSKPVKVKVKGQIAHNHACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVD
EEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCEEEEEC
ASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVEDGWSPPGFTAFVSSIIESGV
HHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEECHHHHHHCCCCCCCHHHHHHHHHHCCC
DPKRMDGIRAQLRTIGLEPYDCLNPGLMDYIATWTAKKSGALPA
CHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRASRSVASWFTEASHTPPTDRPATSVVQNWEEKRMAEKLHPRIDNGLPKESASFAGGTL
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEE
VCACTSKPVKVKVKGQIAHNHACGCTKCWKPEGAIFSVVAVAGTGDVTVTENGDKLKVVD
EEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCEEEECCCCEEEEEC
ASALIQRHACTGCGVHMHGPVERDHAFKGLTFIHPERFVEDGWSPPGFTAFVSSIIESGV
HHHHHHHHCCCCCCEEECCCCCHHHHHCCCEEECHHHHHHCCCCCCCHHHHHHHHHHCCC
DPKRMDGIRAQLRTIGLEPYDCLNPGLMDYIATWTAKKSGALPA
CHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]