Click here to switch to the map view.

The map label for this gene is adhI [H]

Identifier: 13471013

GI number: 13471013

Start: 701568

End: 702695

Strand: Direct

Name: adhI [H]

Synonym: mlr0872

Alternate gene names: 13471013

Gene position: 701568-702695 (Clockwise)

Preceding gene: 13471009

Following gene: 13471014

Centisome position: 9.97

GC content: 64.01

Gene sequence:

>1128_bases
ATGAAGACCCGCGCCGCTGTCGCTGTCGCCGCCGGAAAGCCGCTGGAGATCATGGAGGTCGACCTCGACGGTCCGCGCGA
AGGCGAGGTTCTGGTCGAGATCAAGGCGACCGGCATCTGCCACACCGACGAGTTCACCTTGTCGGGCGCCGATCCCGAAG
GGCTGTTCCCGGCCATTCTCGGCCATGAGGGCGCCGGTGTCGTGGTCGATGTCGGCAAGGGTGTCACCTCGGTCAAGAAG
GGCGACCATGTCATCCCGCTCTATACGCCGGAATGCCGGCAGTGCCCGTCCTGCTTGTCGAGAAAGACCAATCTGTGCAC
GGCGATCCGCGCCACGCAGGGGCAAGGCTTGATGCCGGACGGCTCGTCGCGCTTCTCGATCGGCAAGGACAAGATCTTTC
ACTATATGGGCTGCTCGACCTTCTCCAACTTCACCGTTCTGCCCGAGATCGCGCTGGCCAAGGTTAACCCGGACGCCCCC
TTCGACAAGATCTGCTACATCGGCTGCGGCGTCACCACCGGCATCGGCGCCGTCATCAACACCGCCAAGGTTGAGCAAGG
CGCGACCGCTGTCGTCTTCGGCCTCGGCGGCATCGGCCTCAACGTCATCCAGGGCCTGAAGCTCGCCGGTGCCGACATGA
TCATCGGCGTCGACCTCAACAACGACAAGAAGGCCTGGGGCGAGCGGTTCGGCATGACGCATTTCGTCAATCCGAAGGAG
ATCGACGGCGACATCGTCCCTCACCTCGTCAACATGACCAAGCGCGGCGCCGACCAGATCGGCGGCGCCGACTACACGTT
CGACTGCACCGGCAACACCAAGGTGATGCGCCAGGCGCTGGAAGCCTCGCACCGCGGTTGGGGCAAGTCGGTCGTCATCG
GCGTCGCCGGCGCCGGCCAGGAGATCTCGACGCGTCCGTTCCAGCTGGTCACCGGTCGCACCTGGATGGGCACCGCCTTC
GGCGGCGCGCGCGGCCGCACCGACGTGCCGCGGATCGTCGACTGGTACATGGACGGCAAGATCCAGATCGATCCGATGAT
CACCCATTTGCTGAAGCTGGAAGACATCAACAAGGGGTTCGACCTCATGCATGAGGGCAAGTCGATCCGTAGCGTCGTGG
TTTACTGA

Upstream 100 bases:

>100_bases
TCCAATTCATCGCTGCGGCCCAGTCTCGGCGCCAGCTTCCAGTTGATCGTGTCGATAAAACCTGAAACCTTGGAAAAAAA
TTCGCATTGGAGGACGCCTG

Downstream 100 bases:

>100_bases
AGCAAGCCACACGCCGCCGACCGACCGGCCTGCGACCTCTGTGGTTCAAAACTGGGAGGAGAAAAGAATGGCGGAAAAAC
TGCATCCGAGAATCGACAAT

Product: glutathione dependent formaldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKTRAAVAVAAGKPLEIMEVDLDGPREGEVLVEIKATGICHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGSSRFSIGKDKIFHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEQGATAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNDKKAWGERFGMTHFVNPKE
IDGDIVPHLVNMTKRGADQIGGADYTFDCTGNTKVMRQALEASHRGWGKSVVIGVAGAGQEISTRPFQLVTGRTWMGTAF
GGARGRTDVPRIVDWYMDGKIQIDPMITHLLKLEDINKGFDLMHEGKSIRSVVVY

Sequences:

>Translated_375_residues
MKTRAAVAVAAGKPLEIMEVDLDGPREGEVLVEIKATGICHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGSSRFSIGKDKIFHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEQGATAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNDKKAWGERFGMTHFVNPKE
IDGDIVPHLVNMTKRGADQIGGADYTFDCTGNTKVMRQALEASHRGWGKSVVIGVAGAGQEISTRPFQLVTGRTWMGTAF
GGARGRTDVPRIVDWYMDGKIQIDPMITHLLKLEDINKGFDLMHEGKSIRSVVVY
>Mature_375_residues
MKTRAAVAVAAGKPLEIMEVDLDGPREGEVLVEIKATGICHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGSSRFSIGKDKIFHYMGCSTFSNFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEQGATAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNDKKAWGERFGMTHFVNPKE
IDGDIVPHLVNMTKRGADQIGGADYTFDCTGNTKVMRQALEASHRGWGKSVVIGVAGAGQEISTRPFQLVTGRTWMGTAF
GGARGRTDVPRIVDWYMDGKIQIDPMITHLLKLEDINKGFDLMHEGKSIRSVVVY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=58.1769436997319, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=43.421052631579, Blast_Score=328, Evalue=6e-90,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=43.2, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=43.4666666666667, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=42.9333333333333, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI71743840, Length=376, Percent_Identity=42.5531914893617, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI262073058, Length=376, Percent_Identity=42.5531914893617, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI156523966, Length=374, Percent_Identity=40.9090909090909, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI4501939, Length=367, Percent_Identity=41.1444141689373, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=60, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082125, Length=389, Percent_Identity=26.4781491002571, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787863, Length=355, Percent_Identity=23.6619718309859, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI226510992, Length=381, Percent_Identity=24.1469816272966, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790045, Length=356, Percent_Identity=26.123595505618, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788075, Length=377, Percent_Identity=25.9946949602122, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786825, Length=368, Percent_Identity=24.1847826086957, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081918, Length=366, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25146526, Length=376, Percent_Identity=53.9893617021277, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=378, Percent_Identity=53.7037037037037, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=346, Percent_Identity=54.3352601156069, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562584, Length=366, Percent_Identity=28.9617486338798, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=374, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=377, Percent_Identity=28.1167108753316, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=377, Percent_Identity=54.1114058355438, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319621, Length=379, Percent_Identity=27.9683377308707, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=27.1708683473389, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323099, Length=384, Percent_Identity=25.78125, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=27.1708683473389, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=379, Percent_Identity=24.2744063324538, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=366, Percent_Identity=24.3169398907104, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=372, Percent_Identity=25.2688172043011, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323729, Length=367, Percent_Identity=24.5231607629428, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=56.4171122994652, Blast_Score=439, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39994; Mature: 39994

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAVAAGKPLEIMEVDLDGPREGEVLVEIKATGICHTDEFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GSSRFSIGKDKIFHYMGCSTFSNFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCCCCCCHHHHEEEECCCCCCCCEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHH
TAKVEQGATAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNDKKAWGERFGMTHFVNPKE
HHECCCCCEEEEEECCCCHHHHHHCCEECCCEEEEEEECCCCHHHHHHHCCCEECCCHHH
IDGDIVPHLVNMTKRGADQIGGADYTFDCTGNTKVMRQALEASHRGWGKSVVIGVAGAGQ
CCCCHHHHHHHHHHHCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EISTRPFQLVTGRTWMGTAFGGARGRTDVPRIVDWYMDGKIQIDPMITHLLKLEDINKGF
CCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHHHEECCEEEECHHHHHHHHHHHCCCCH
DLMHEGKSIRSVVVY
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MKTRAAVAVAAGKPLEIMEVDLDGPREGEVLVEIKATGICHTDEFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GSSRFSIGKDKIFHYMGCSTFSNFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCCCCCCHHHHEEEECCCCCCCCEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHH
TAKVEQGATAVVFGLGGIGLNVIQGLKLAGADMIIGVDLNNDKKAWGERFGMTHFVNPKE
HHECCCCCEEEEEECCCCHHHHHHCCEECCCEEEEEEECCCCHHHHHHHCCCEECCCHHH
IDGDIVPHLVNMTKRGADQIGGADYTFDCTGNTKVMRQALEASHRGWGKSVVIGVAGAGQ
CCCCHHHHHHHHHHHCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EISTRPFQLVTGRTWMGTAFGGARGRTDVPRIVDWYMDGKIQIDPMITHLLKLEDINKGF
CCCCCCEEEEECCEEEEECCCCCCCCCCCCHHHHHEECCEEEECHHHHHHHHHHHCCCCH
DLMHEGKSIRSVVVY
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]