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The map label for this gene is lutR [H]

Identifier: 13471000

GI number: 13471000

Start: 683342

End: 684124

Strand: Reverse

Name: lutR [H]

Synonym: mll0857

Alternate gene names: 13471000

Gene position: 684124-683342 (Counterclockwise)

Preceding gene: 13471001

Following gene: 13470999

Centisome position: 9.72

GC content: 63.73

Gene sequence:

>783_bases
ATGCAGAAATTGGTTGGACCATTGGACCACTTGAATTCGGGAGGATTTGTGGACCAGAACAGCCTGCCACCCATTCAGAC
GACGGCGCGCGATGACGCCGTGCTGAAGGCGTTGGTGGGCTTTGTTCGCGCCGAGGCCCTGCAGCCCGGCAATCGGCTTC
CGACCGAACGCATCCTGGCCGAGCGGCTGAAAGTCAGCCGCAACACAGTGCGCGAGGCGCTGACGCGGTGGGAGGGGCTT
GGCCTGGTCGAGCGCCGGCAAGGCAGCGGCACCTATCTCAAGGCGGCTGTCTCGCCCGACATGCTGCATATGCCGCTGAC
GCTTGCAGGCGGCAACGACTTCACCAGCCTGATGCATACGCTTGAAATTCGCCGCGCGCTGGAAGCGGAGGCGGCGGCCC
TTTGCGCCGAACGCGCCAGCCCGGCGGACATTGCCGAGATCAAGCGCAAGCTCGACATCATGGAGCAGGCCTTCCGCACC
CGCGACGGCATGTCGTCGGAAGAGGACTGGGAGTTCCACCAGGCGATCTACCGGGTCTCCGGCAATCCGCTGTTCGAGCA
GATCATCGCAGCCATGCACGAACTGTTCCATCGCTTCTGGGAGCATCCGCTCGGCGTGCGTGATTTCGGCCACGCCAGCT
TTCCCTATCACCGCACCATCTACGAATGCATTGCCGCACGCGACCCGCAAGGCGCCCGCGCCGAGGCGCTCAAGCTGATC
GCCACCGTCGAGGACGATCTCAAGCGCGGTGCGGCCAAACTCAAACTTTCGAGCCAGACATGA

Upstream 100 bases:

>100_bases
AGGCGGACCACGCGATGTCCTCAAACGCATCGCGTGGCAGCTTGCGACAAGGCAAAAGCCGACTGCTCTCCTGACAATCG
GTTGACAGGAGGTTTTATCT

Downstream 100 bases:

>100_bases
ACGAGCATCCGAGCGGTTTCGATCACGATTATCTTGCCGAGGCGGCGACGCTTTTGGCGCATGACGAGCCGTTCCCGGGT
GGCGCGGTGGTGCCGCCGAT

Product: transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQKLVGPLDHLNSGGFVDQNSLPPIQTTARDDAVLKALVGFVRAEALQPGNRLPTERILAERLKVSRNTVREALTRWEGL
GLVERRQGSGTYLKAAVSPDMLHMPLTLAGGNDFTSLMHTLEIRRALEAEAAALCAERASPADIAEIKRKLDIMEQAFRT
RDGMSSEEDWEFHQAIYRVSGNPLFEQIIAAMHELFHRFWEHPLGVRDFGHASFPYHRTIYECIAARDPQGARAEALKLI
ATVEDDLKRGAAKLKLSSQT

Sequences:

>Translated_260_residues
MQKLVGPLDHLNSGGFVDQNSLPPIQTTARDDAVLKALVGFVRAEALQPGNRLPTERILAERLKVSRNTVREALTRWEGL
GLVERRQGSGTYLKAAVSPDMLHMPLTLAGGNDFTSLMHTLEIRRALEAEAAALCAERASPADIAEIKRKLDIMEQAFRT
RDGMSSEEDWEFHQAIYRVSGNPLFEQIIAAMHELFHRFWEHPLGVRDFGHASFPYHRTIYECIAARDPQGARAEALKLI
ATVEDDLKRGAAKLKLSSQT
>Mature_260_residues
MQKLVGPLDHLNSGGFVDQNSLPPIQTTARDDAVLKALVGFVRAEALQPGNRLPTERILAERLKVSRNTVREALTRWEGL
GLVERRQGSGTYLKAAVSPDMLHMPLTLAGGNDFTSLMHTLEIRRALEAEAAALCAERASPADIAEIKRKLDIMEQAFRT
RDGMSSEEDWEFHQAIYRVSGNPLFEQIIAAMHELFHRFWEHPLGVRDFGHASFPYHRTIYECIAARDPQGARAEALKLI
ATVEDDLKRGAAKLKLSSQT

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=226, Percent_Identity=28.7610619469027, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790780, Length=217, Percent_Identity=29.9539170506912, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786303, Length=221, Percent_Identity=28.5067873303167, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=207, Percent_Identity=27.0531400966184, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789353, Length=218, Percent_Identity=28.4403669724771, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29022; Mature: 29022

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50949 HTH_GNTR ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLVGPLDHLNSGGFVDQNSLPPIQTTARDDAVLKALVGFVRAEALQPGNRLPTERILA
CCCHHCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ERLKVSRNTVREALTRWEGLGLVERRQGSGTYLKAAVSPDMLHMPLTLAGGNDFTSLMHT
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCEECCEEEECCCHHHHHHHH
LEIRRALEAEAAALCAERASPADIAEIKRKLDIMEQAFRTRDGMSSEEDWEFHQAIYRVS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
GNPLFEQIIAAMHELFHRFWEHPLGVRDFGHASFPYHRTIYECIAARDPQGARAEALKLI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
ATVEDDLKRGAAKLKLSSQT
HHHHHHHHCCHHHCCCCCCC
>Mature Secondary Structure
MQKLVGPLDHLNSGGFVDQNSLPPIQTTARDDAVLKALVGFVRAEALQPGNRLPTERILA
CCCHHCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
ERLKVSRNTVREALTRWEGLGLVERRQGSGTYLKAAVSPDMLHMPLTLAGGNDFTSLMHT
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCEECCEEEECCCHHHHHHHH
LEIRRALEAEAAALCAERASPADIAEIKRKLDIMEQAFRTRDGMSSEEDWEFHQAIYRVS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
GNPLFEQIIAAMHELFHRFWEHPLGVRDFGHASFPYHRTIYECIAARDPQGARAEALKLI
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
ATVEDDLKRGAAKLKLSSQT
HHHHHHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]