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The map label for this gene is ycdT [H]

Identifier: 13470977

GI number: 13470977

Start: 653485

End: 654741

Strand: Direct

Name: ycdT [H]

Synonym: mlr0825

Alternate gene names: 13470977

Gene position: 653485-654741 (Clockwise)

Preceding gene: 13470974

Following gene: 13470981

Centisome position: 9.29

GC content: 61.73

Gene sequence:

>1257_bases
GTGGAAGTCGTGCGCATTAAGGAAACTTTAGCGCTACCGCATCTATGGTCACACCAAGCGAAGATGGCGGGTGGGGACGC
GTCTCTTCGCAAGACGTTCTGGGCTATCGGGTCGCGCTCCCCATGTCGGCGATCAACGGAACCCGCAAATTCCGCGCTGG
GATCCGAGGGGAAGAGTGGATCAGACATGCAGCCGGCAGCCGCCACGACCGAACGAAGCACCGACATCGCAGCCACGGTT
GTTGCCACCATGCGCCAGCTTGGCGTCCTCGGCCTGCCGCGCAACTACGAAATCTTCTACGAGGCATTGAGCGGCACCAA
CCGCGAGCTCAGCCTCGCCGTCGTGTCACTGAGCAGCCGGCCGACGCAGGATGAGCTGGATAAGATCGGACGCGCCTTCT
TTGCCCAGAACCACGGCCCCGGCATCGTCGAGCATGCGCGGGACGTCATCGCCCGGGAACTCGAGGAGGTCGCGGCGCTG
CTGCGCAGCGAACGCAGCCACATCGAAAAATACGGCAGGATCCTCGACGAGACATCGAACGGCCTGAGTGGCCGCAGCCT
GCTCTCCCAGGATCTCCTGCAAAAAATCGCCAACGCCATGGCCGTTGCCACCAATTCGACGATCGATCACGGCAAGCAGA
TCGCCTCGACGCTCAGCGACAAGACGGCCGAACTCGAAAGCGTCAAGTCGAAGCTGGAGGAATACAAGCGGCTGGCCGAT
ACGGATCCGCTGACCCAGATCCGGAACCGCCGCGCCTTCGACAAGGAAATCACCCGGATCTACAACAGCAACAAGGGTAT
CCTGTTCAATGCTTTGATCCTTGCCGACATCGATCGGTTCAAGGACATCAACGACCGCTACGGCCATCCTGTCGGCGACA
AGATCATCCAGATCATCGCCGAGATTTTCCAGACCAGCATTCGCGGCGACATGTTCGTCGCCCGCACCGGCGGCGAGGAG
TTCGCGCTGATCATCGAGGGCGCCAGCGAAGACGCCACCTACGAGATCGCCGAGCGCATTCGCGCCCTGATCGAGCAGAC
GCCGTTCACCAGCAGCCAGACCGGCATGAATTACGGAACCGTCACCGTCTCGATGGGCATCTGCATGGCATCCGAGGCCG
AAGGCCCGGAAGACCTCTACACCAAGGCCGACCGGGCGCTCTACCGTTCGAAAGTGAGCGGCCGCAACCGCGTCACCAAG
CATTCGACGATGGCCGGCCGCGCCGGCAAGAGCTGGCTGCTCTACAAGAAGGACTGA

Upstream 100 bases:

>100_bases
ACAACACGCGGATCTCGAAGTCAAAACCGCCAAACTGGGTGTTGTCCCGCGACGGTGGTACAAAATATATGCTTCTACAG
CCATAGGTAGAGAAAAACCT

Downstream 100 bases:

>100_bases
CGCCTTCTATCAGCTGATCGCGCTGGCAGCTGCCGAGAACACAAACGTGAGCTAAAACGAAGAAGGCGCCGGATTTCTCC
AGCGCCTTTCCTTAATCGGG

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MEVVRIKETLALPHLWSHQAKMAGGDASLRKTFWAIGSRSPCRRSTEPANSALGSEGKSGSDMQPAAATTERSTDIAATV
VATMRQLGVLGLPRNYEIFYEALSGTNRELSLAVVSLSSRPTQDELDKIGRAFFAQNHGPGIVEHARDVIARELEEVAAL
LRSERSHIEKYGRILDETSNGLSGRSLLSQDLLQKIANAMAVATNSTIDHGKQIASTLSDKTAELESVKSKLEEYKRLAD
TDPLTQIRNRRAFDKEITRIYNSNKGILFNALILADIDRFKDINDRYGHPVGDKIIQIIAEIFQTSIRGDMFVARTGGEE
FALIIEGASEDATYEIAERIRALIEQTPFTSSQTGMNYGTVTVSMGICMASEAEGPEDLYTKADRALYRSKVSGRNRVTK
HSTMAGRAGKSWLLYKKD

Sequences:

>Translated_418_residues
MEVVRIKETLALPHLWSHQAKMAGGDASLRKTFWAIGSRSPCRRSTEPANSALGSEGKSGSDMQPAAATTERSTDIAATV
VATMRQLGVLGLPRNYEIFYEALSGTNRELSLAVVSLSSRPTQDELDKIGRAFFAQNHGPGIVEHARDVIARELEEVAAL
LRSERSHIEKYGRILDETSNGLSGRSLLSQDLLQKIANAMAVATNSTIDHGKQIASTLSDKTAELESVKSKLEEYKRLAD
TDPLTQIRNRRAFDKEITRIYNSNKGILFNALILADIDRFKDINDRYGHPVGDKIIQIIAEIFQTSIRGDMFVARTGGEE
FALIIEGASEDATYEIAERIRALIEQTPFTSSQTGMNYGTVTVSMGICMASEAEGPEDLYTKADRALYRSKVSGRNRVTK
HSTMAGRAGKSWLLYKKD
>Mature_418_residues
MEVVRIKETLALPHLWSHQAKMAGGDASLRKTFWAIGSRSPCRRSTEPANSALGSEGKSGSDMQPAAATTERSTDIAATV
VATMRQLGVLGLPRNYEIFYEALSGTNRELSLAVVSLSSRPTQDELDKIGRAFFAQNHGPGIVEHARDVIARELEEVAAL
LRSERSHIEKYGRILDETSNGLSGRSLLSQDLLQKIANAMAVATNSTIDHGKQIASTLSDKTAELESVKSKLEEYKRLAD
TDPLTQIRNRRAFDKEITRIYNSNKGILFNALILADIDRFKDINDRYGHPVGDKIIQIIAEIFQTSIRGDMFVARTGGEE
FALIIEGASEDATYEIAERIRALIEQTPFTSSQTGMNYGTVTVSMGICMASEAEGPEDLYTKADRALYRSKVSGRNRVTK
HSTMAGRAGKSWLLYKKD

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=193, Percent_Identity=37.3056994818653, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=37.2670807453416, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787802, Length=176, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=266, Percent_Identity=28.9473684210526, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=36.3095238095238, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=35.1190476190476, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788085, Length=202, Percent_Identity=32.1782178217822, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=238, Percent_Identity=26.890756302521, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786584, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=33.7423312883436, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=29.7619047619048, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788956, Length=199, Percent_Identity=31.1557788944724, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=27.3291925465839, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46050; Mature: 46050

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVRIKETLALPHLWSHQAKMAGGDASLRKTFWAIGSRSPCRRSTEPANSALGSEGKSG
CCEEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCC
SDMQPAAATTERSTDIAATVVATMRQLGVLGLPRNYEIFYEALSGTNRELSLAVVSLSSR
CCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCC
PTQDELDKIGRAFFAQNHGPGIVEHARDVIARELEEVAALLRSERSHIEKYGRILDETSN
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLSGRSLLSQDLLQKIANAMAVATNSTIDHGKQIASTLSDKTAELESVKSKLEEYKRLAD
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDPLTQIRNRRAFDKEITRIYNSNKGILFNALILADIDRFKDINDRYGHPVGDKIIQIIA
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHH
EIFQTSIRGDMFVARTGGEEFALIIEGASEDATYEIAERIRALIEQTPFTSSQTGMNYGT
HHHHHHHCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VTVSMGICMASEAEGPEDLYTKADRALYRSKVSGRNRVTKHSTMAGRAGKSWLLYKKD
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure
MEVVRIKETLALPHLWSHQAKMAGGDASLRKTFWAIGSRSPCRRSTEPANSALGSEGKSG
CCEEEEHHHHHCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCC
SDMQPAAATTERSTDIAATVVATMRQLGVLGLPRNYEIFYEALSGTNRELSLAVVSLSSR
CCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCC
PTQDELDKIGRAFFAQNHGPGIVEHARDVIARELEEVAALLRSERSHIEKYGRILDETSN
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLSGRSLLSQDLLQKIANAMAVATNSTIDHGKQIASTLSDKTAELESVKSKLEEYKRLAD
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TDPLTQIRNRRAFDKEITRIYNSNKGILFNALILADIDRFKDINDRYGHPVGDKIIQIIA
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHH
EIFQTSIRGDMFVARTGGEEFALIIEGASEDATYEIAERIRALIEQTPFTSSQTGMNYGT
HHHHHHHCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCE
VTVSMGICMASEAEGPEDLYTKADRALYRSKVSGRNRVTKHSTMAGRAGKSWLLYKKD
EEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]