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The map label for this gene is recG [H]

Identifier: 13470981

GI number: 13470981

Start: 660366

End: 662474

Strand: Direct

Name: recG [H]

Synonym: mlr0830

Alternate gene names: 13470981

Gene position: 660366-662474 (Clockwise)

Preceding gene: 13470977

Following gene: 13470984

Centisome position: 9.39

GC content: 67.61

Gene sequence:

>2109_bases
ATGCGTCCTTCCATCCTCGATCCGCTGTTTGTTCCGATCACTTCGCTTGCCGGCGTCGGACCGAAGGTCGGCGCGCTGAT
CGAGAAGGTCGTCACGGCTGATCTTGGTGATCGCGCGGCCCGCGCGGGCGACCTTCTGTTCGTGCTGCCGCATACCGTTA
TCGACCGCCGCAGCCGGCCGGGCATCGCCGGTTCCGCGGAAGGCCAGATCGTTACCCTCGACGTGCGCATCGACCGCCAC
CAGCCGCCGCCACGCGGCAACCGGTCGGTGCCCTACAGGGTCTACGCCCATGACGATACCGGCGAAATCGGCCTGACCTT
TTTCCATGCGCACGCCGCCTATCTCGAAAAGGCAATGCCCGTCGGCGAGCATGTCGTGGTCTCGGGCCGCATGGAATGGT
TCAACGGCCGCCCGACCATGGTCCACCCCGACCACATCGCGTTGGCCAGCGAGGCGGAAAACCTGCCACTGGTCGAGCCG
GTCTATCCGCTCACCGCGGGTCTCTCCGGCAAGGTGCTGCGCCGTGCGATCGGCCAGGCGTTGGCGCGGCTGCCCGAATT
GCCGGAGTGGCAGGACGTCGCCTTCATGCGCCGGCACACCTTTCCCACCTTTGCCGACGCACTCACCCGCATCCACAATC
CAGCCGACCCGATCGATGTTTCCATCGAGGGTGCGGCCTGGCGGCGTCTTGCCTATGACGAGTTGCTGGCGGGCCAGGTC
TCGCTGGCGCTGGTGCGGGCAAAGATCCGCCGCCTGTCCGGCCGCCCCCTGGTCGGCGACGGCCGCATCGTCGAGAAACT
GCGCGCAGCCCTTCCCTATTCGCTGACCGCAAGCCAGGAATTCGCGCTGGCCGAAATCAATGCCGACCTTGCCGACCCCG
AGCGCATGCTGCGGCTGCTGCAGGGCGATGTCGGATCGGGCAAGACGGTGGTCGCGCTGCTTGCCATGGCGCGCGCCGTC
GAGGCCGGCGGCCAGGCAGCGCTGATGGCGCCGACCGAAATCCTGGCGCGCCAGCACCTGGCGACGATCGCCCCGTTCGC
CGCCAAGGTCGGGCTGCGCATCGCCATCCTCACCGGCCGCGAAAAGGGCCGCGAACGCACCGAAACACTGGCCGGCCTGG
CCAGCGGCGAGATCGACATCGTCGTCGGCACCCACGCGCTGTTCCAGGAGACGGTCACCTTCCACGATCTGGTGCTGGCT
GTCATCGACGAGCAGCATCGCTTCGGCGTTCACCAGCGGCTCGCCATCACCGCCAAGGGCGATGCGCCCGACATGCTGGT
GATGACCGCCACACCGATCCCGCGCACGCTGGTGCTGACCGCCTTCGGCGACATGGACGTCTCCAAGCTGACGGAAAAAC
CGGCCGGCCGACAGCCGATCCGCACCGTCACGCTGCCGCTGGAGCGGCTCGACGAACTGGTCGCCCGCATGCAGGATGCC
GTCGCCGGCGGCCAGAAAATCTACTGGATCTGCCCGCTGGTCGAGGAATCCGAAGAGATCAAGCTGATGTCGGCCGAGGA
TCGCTTTGCCTCGCTGAAACCGCTGTTCGGCGACCGCATCGGCCTTGTCCACGGCCGCATGAAGGGCGCCGAGAAGGACG
AGGCGATGCGCGCCTTCAAGGAGGGCGAAACCCGCATCCTGATCGCCACCACGGTCATCGAGGTCGGCGTCGATGTTCCC
GACGCCACCGTCATGGTCATCGAGCATGCCGAGCGTTTCGGCCTCGCACAGCTGCACCAGCTGCGCGGGCGGGTCGGGCG
CGGCGACAAGCCCTCCTCTTGCGTGCTGCTCTACAAGGACCCGCTCGGCGAGACGGCAAAACGCCGGCTGTCGGTGATGC
GCGAGACCGAAGACGGCTTTTTGATCGCCGAGGAAGACCTCAAGCTGCGCGGTGAAGGCGAGCTGTTGGGCACCCGCCAG
TCCGGCACACCGGGTTTCCAGGTGGCGCGCATCGAGGCGCATGCCGATCTTCTGGAGGCCGCCCGCGACGATGCCAGGCT
TATCCTGTCGCGCGACCCGGAGCTGCAATCCGACCGCGGCGAGGCGCTGCGCCTGCTGCTCTATCTGTTCGGCCGCGACG
AGGCCGTGCGGCTCCTGCGGGCCGGGTGA

Upstream 100 bases:

>100_bases
CCGGTCATTGCGGCGCATCCTCACTTGCTGCGGCGCATATAGCGTGGATAGAAGGGGCTGTCAGCCTTGCGCTGTAGCCG
TCGCGACATAAGCTGACATC

Downstream 100 bases:

>100_bases
GACCCTAATGGCGGATTGGCGACCGTTCCGTTGATCGGCTCGCCATTCAGCGTCACCATCTTGCTTTTCACCTCCGGCGC
CACCAGCCCTGCAGAAACAA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MRPSILDPLFVPITSLAGVGPKVGALIEKVVTADLGDRAARAGDLLFVLPHTVIDRRSRPGIAGSAEGQIVTLDVRIDRH
QPPPRGNRSVPYRVYAHDDTGEIGLTFFHAHAAYLEKAMPVGEHVVVSGRMEWFNGRPTMVHPDHIALASEAENLPLVEP
VYPLTAGLSGKVLRRAIGQALARLPELPEWQDVAFMRRHTFPTFADALTRIHNPADPIDVSIEGAAWRRLAYDELLAGQV
SLALVRAKIRRLSGRPLVGDGRIVEKLRAALPYSLTASQEFALAEINADLADPERMLRLLQGDVGSGKTVVALLAMARAV
EAGGQAALMAPTEILARQHLATIAPFAAKVGLRIAILTGREKGRERTETLAGLASGEIDIVVGTHALFQETVTFHDLVLA
VIDEQHRFGVHQRLAITAKGDAPDMLVMTATPIPRTLVLTAFGDMDVSKLTEKPAGRQPIRTVTLPLERLDELVARMQDA
VAGGQKIYWICPLVEESEEIKLMSAEDRFASLKPLFGDRIGLVHGRMKGAEKDEAMRAFKEGETRILIATTVIEVGVDVP
DATVMVIEHAERFGLAQLHQLRGRVGRGDKPSSCVLLYKDPLGETAKRRLSVMRETEDGFLIAEEDLKLRGEGELLGTRQ
SGTPGFQVARIEAHADLLEAARDDARLILSRDPELQSDRGEALRLLLYLFGRDEAVRLLRAG

Sequences:

>Translated_702_residues
MRPSILDPLFVPITSLAGVGPKVGALIEKVVTADLGDRAARAGDLLFVLPHTVIDRRSRPGIAGSAEGQIVTLDVRIDRH
QPPPRGNRSVPYRVYAHDDTGEIGLTFFHAHAAYLEKAMPVGEHVVVSGRMEWFNGRPTMVHPDHIALASEAENLPLVEP
VYPLTAGLSGKVLRRAIGQALARLPELPEWQDVAFMRRHTFPTFADALTRIHNPADPIDVSIEGAAWRRLAYDELLAGQV
SLALVRAKIRRLSGRPLVGDGRIVEKLRAALPYSLTASQEFALAEINADLADPERMLRLLQGDVGSGKTVVALLAMARAV
EAGGQAALMAPTEILARQHLATIAPFAAKVGLRIAILTGREKGRERTETLAGLASGEIDIVVGTHALFQETVTFHDLVLA
VIDEQHRFGVHQRLAITAKGDAPDMLVMTATPIPRTLVLTAFGDMDVSKLTEKPAGRQPIRTVTLPLERLDELVARMQDA
VAGGQKIYWICPLVEESEEIKLMSAEDRFASLKPLFGDRIGLVHGRMKGAEKDEAMRAFKEGETRILIATTVIEVGVDVP
DATVMVIEHAERFGLAQLHQLRGRVGRGDKPSSCVLLYKDPLGETAKRRLSVMRETEDGFLIAEEDLKLRGEGELLGTRQ
SGTPGFQVARIEAHADLLEAARDDARLILSRDPELQSDRGEALRLLLYLFGRDEAVRLLRAG
>Mature_702_residues
MRPSILDPLFVPITSLAGVGPKVGALIEKVVTADLGDRAARAGDLLFVLPHTVIDRRSRPGIAGSAEGQIVTLDVRIDRH
QPPPRGNRSVPYRVYAHDDTGEIGLTFFHAHAAYLEKAMPVGEHVVVSGRMEWFNGRPTMVHPDHIALASEAENLPLVEP
VYPLTAGLSGKVLRRAIGQALARLPELPEWQDVAFMRRHTFPTFADALTRIHNPADPIDVSIEGAAWRRLAYDELLAGQV
SLALVRAKIRRLSGRPLVGDGRIVEKLRAALPYSLTASQEFALAEINADLADPERMLRLLQGDVGSGKTVVALLAMARAV
EAGGQAALMAPTEILARQHLATIAPFAAKVGLRIAILTGREKGRERTETLAGLASGEIDIVVGTHALFQETVTFHDLVLA
VIDEQHRFGVHQRLAITAKGDAPDMLVMTATPIPRTLVLTAFGDMDVSKLTEKPAGRQPIRTVTLPLERLDELVARMQDA
VAGGQKIYWICPLVEESEEIKLMSAEDRFASLKPLFGDRIGLVHGRMKGAEKDEAMRAFKEGETRILIATTVIEVGVDVP
DATVMVIEHAERFGLAQLHQLRGRVGRGDKPSSCVLLYKDPLGETAKRRLSVMRETEDGFLIAEEDLKLRGEGELLGTRQ
SGTPGFQVARIEAHADLLEAARDDARLILSRDPELQSDRGEALRLLLYLFGRDEAVRLLRAG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=693, Percent_Identity=41.4141414141414, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1787357, Length=418, Percent_Identity=38.9952153110048, Blast_Score=251, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76660; Mature: 76660

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSILDPLFVPITSLAGVGPKVGALIEKVVTADLGDRAARAGDLLFVLPHTVIDRRSRP
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEECCHHHHCCCCCC
GIAGSAEGQIVTLDVRIDRHQPPPRGNRSVPYRVYAHDDTGEIGLTFFHAHAAYLEKAMP
CCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCC
VGEHVVVSGRMEWFNGRPTMVHPDHIALASEAENLPLVEPVYPLTAGLSGKVLRRAIGQA
CCCEEEEECCEEEECCCCCEECCCCEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
LARLPELPEWQDVAFMRRHTFPTFADALTRIHNPADPIDVSIEGAAWRRLAYDELLAGQV
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
SLALVRAKIRRLSGRPLVGDGRIVEKLRAALPYSLTASQEFALAEINADLADPERMLRLL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHH
QGDVGSGKTVVALLAMARAVEAGGQAALMAPTEILARQHLATIAPFAAKVGLRIAILTGR
HCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
EKGRERTETLAGLASGEIDIVVGTHALFQETVTFHDLVLAVIDEQHRFGVHQRLAITAKG
CCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEECC
DAPDMLVMTATPIPRTLVLTAFGDMDVSKLTEKPAGRQPIRTVTLPLERLDELVARMQDA
CCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
VAGGQKIYWICPLVEESEEIKLMSAEDRFASLKPLFGDRIGLVHGRMKGAEKDEAMRAFK
HCCCCEEEEEECCCCCCCCEEEEECCCHHHHCCCHHCCCEEEEECCCCCCCHHHHHHHHH
EGETRILIATTVIEVGVDVPDATVMVIEHAERFGLAQLHQLRGRVGRGDKPSSCVLLYKD
CCCCEEEEEEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEC
PLGETAKRRLSVMRETEDGFLIAEEDLKLRGEGELLGTRQSGTPGFQVARIEAHADLLEA
CCCHHHHHHHHHHHCCCCCEEEEECCCEEECCCCEEEECCCCCCCEEEEEEHHHHHHHHH
ARDDARLILSRDPELQSDRGEALRLLLYLFGRDEAVRLLRAG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MRPSILDPLFVPITSLAGVGPKVGALIEKVVTADLGDRAARAGDLLFVLPHTVIDRRSRP
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCEEEECCHHHHCCCCCC
GIAGSAEGQIVTLDVRIDRHQPPPRGNRSVPYRVYAHDDTGEIGLTFFHAHAAYLEKAMP
CCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEEHHHHHHHHHCCC
VGEHVVVSGRMEWFNGRPTMVHPDHIALASEAENLPLVEPVYPLTAGLSGKVLRRAIGQA
CCCEEEEECCEEEECCCCCEECCCCEEECCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
LARLPELPEWQDVAFMRRHTFPTFADALTRIHNPADPIDVSIEGAAWRRLAYDELLAGQV
HHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
SLALVRAKIRRLSGRPLVGDGRIVEKLRAALPYSLTASQEFALAEINADLADPERMLRLL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEECCCCCEEEEECCCCCCCHHHHHHHH
QGDVGSGKTVVALLAMARAVEAGGQAALMAPTEILARQHLATIAPFAAKVGLRIAILTGR
HCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
EKGRERTETLAGLASGEIDIVVGTHALFQETVTFHDLVLAVIDEQHRFGVHQRLAITAKG
CCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEECC
DAPDMLVMTATPIPRTLVLTAFGDMDVSKLTEKPAGRQPIRTVTLPLERLDELVARMQDA
CCCCEEEEECCCCCCEEEEEEECCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
VAGGQKIYWICPLVEESEEIKLMSAEDRFASLKPLFGDRIGLVHGRMKGAEKDEAMRAFK
HCCCCEEEEEECCCCCCCCEEEEECCCHHHHCCCHHCCCEEEEECCCCCCCHHHHHHHHH
EGETRILIATTVIEVGVDVPDATVMVIEHAERFGLAQLHQLRGRVGRGDKPSSCVLLYKD
CCCCEEEEEEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEEC
PLGETAKRRLSVMRETEDGFLIAEEDLKLRGEGELLGTRQSGTPGFQVARIEAHADLLEA
CCCHHHHHHHHHHHCCCCCEEEEECCCEEECCCCEEEECCCCCCCEEEEEEHHHHHHHHH
ARDDARLILSRDPELQSDRGEALRLLLYLFGRDEAVRLLRAG
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]