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The map label for this gene is topA

Identifier: 13358155

GI number: 13358155

Start: 738155

End: 740188

Strand: Reverse

Name: topA

Synonym: UU590

Alternate gene names: 13358155

Gene position: 740188-738155 (Counterclockwise)

Preceding gene: 13358156

Following gene: 13358154

Centisome position: 98.47

GC content: 27.34

Gene sequence:

>2034_bases
ATGCAAAAAGATTTAATAGTAGTAGAGTCACCAAATAAAATTAAATCAATTCAGTCTTATTTAGGTGATAGTTATAATGT
AATAGCTACATGTGGACATTTACGTGAGCTCGCAAAAAAATCTGGTTATGATCCAAAAACTTATGATTTAAAATGAGAAA
TAATAAAAACAACAAATAAAAAATTATCGAAAAAACAACAAATAAAAACAATTATTGATTTAGCAAAAAAATCAAATAAT
ATCTATTTAGCAACTGACCCTGATCGTGAAGGTGAAGCTATTAGTTGACATGTTTATGATTTATTACCAAAAAAAGATAA
AGAACGTGTTAAAAGAATAACTTTTAATGAAATAACACAGAAAGCTATTTTAAAATCTATTGATGAAAAACATGATATTG
ATTTTAATTTGGTAGATTCGCAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCTGGATTAGTTAAA
AAAAACTTACATGGCTTATCTGCTGGGCGAGTACAATCGATCGCTTTATTATTTGTAGTTGAGAGAGAGTTAGAAAGACG
TTCTTTTGTTAAAGAAGAATGATATCAAATTGAAGGAATAATTGAAAAAAACATTTTTGTATCTTTAAGAAAAATTCCCT
ATGATGTTGAATTATATAATCAGAAAGATAATAGCGATAGTGATTTAAAATTTCTACACAAAAAAGATGCACAGCGTGTG
ATTGATGATTTAAGTGATATTTTTAAAGTTTATAGTATTGATGATTTGAAAATCACCAAAGGCGATGTTCAATTACCATT
AACAACTGATAAATTATTACAAATCGCATCCAATAATTTTGGTTGAAGTGCTAATAAAACAACTTTAATAGCTCAACAAA
TGTTTGAAGGATTAGAAATTAATAATCAGCAATTAGCTTTAATAACTTATCCAAGAACAGATAGCGAGCGATTAAGTGAT
GATTTTTTACAAGAAGCTAATGCCTTTATTGAAAATAATTTTGGTAAAGAATATCTAGAAAATTTTAAAAATAAAAAAAT
TAATAGAAAAGAAGTAAATATTCAAGACGCACATGAAGCAATTCGCCCTGTTGATATTAATTTAAAACCTGAAGAAATTA
AAGATTATGTTTCATTAGATATTTATAAGCTGTATAACTTAGTTTGAAATAAAACACTATCAGCTTTAATGAAAGTGCCA
ACTTTTAATAAGCAAGTAATTCGTTTTATTAATAATGAATATAAATTTTATACAGCTTATAAAACAATTCGAACTTTAGG
ATATTGAATTTTAGATTTTTATAAAAAGCAACGTGATGAATTAGAATTCCAACCTCCTATAATTAATGTTGGTGATGAAT
TTAAAAAAGAAAAGATCGATTTAATACAAAAAGAAACTACGCCACCGCCTTATTACACAGAAGCCTCTTTAATTGCAGCG
CTAAAAAATGCCGGAGTAGGTCGGCCTTCAACATATGCAACAATGGCAACTATTGGCACCACTAGGGGTTACATCAATAA
AGATAAGAGTAAATTAATTCCAACAGAATTAGGGATTAAAGTCATCCAAGAGTTAAAAGAAGCCTTTCCAAAAGTGATTT
CTATTAAATTTACATCTGAGATGGAAGAACGTTTAGATGTTATAGCTAATGGCAAATTATTTTGAAAAGATTTAGTTAGC
ACTTTTATTCCTATTTTTGAAGAAGAAGTAAAACAAGCATACACTAAAATCGAAAAAGTTCCTGATGAAAAAATTAATCG
ATTATGTCCTATATGTGCTCATGATTTATTGATTAAAAGATCTAGAATGAACACTCGGTTCATTGCGTGTTCAAATTTCC
CTACATGTCGTTTTACAGAATCGTTAGAGAAACCAGAAATTCTTAACGAATTATGTCCTAACTGTTCAAAATCATTGGTT
AAACGTAAAAATCGTAAAAATAAATATTTTATTGGATGCACAGGTTATCCTGAATGTAATTTTATTCGTAATTTAGATGA
ATCAAACAATGATAAAAACGATTTTAATCAATAA

Upstream 100 bases:

>100_bases
TTAAAAATTCAAAATTAATAAATTAAACAACAACTATAAAATATTATAATTATTTACAATTGTTAAAACGAGCAAATAAT
TGTAGTAAATGGGAGAAAAA

Downstream 100 bases:

>100_bases
TAATATAATATTAAAAAGAGGAAAATTTATGTTAATTAAATTAGAAAGTAACCAAAATTTAAATGAATTATTAAAGGAAA
ATCATTCTAAACCAATATTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKWEIIKTTNKKLSKKQQIKTIIDLAKKSNN
IYLATDPDREGEAISWHVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRSFVKEEWYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQRV
IDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGWSANKTTLIAQQMFEGLEINNQQLALITYPRTDSERLSD
DFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLVWNKTLSALMKVP
TFNKQVIRFINNEYKFYTAYKTIRTLGYWILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLFWKDLVS
TFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLV
KRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ

Sequences:

>Translated_677_residues
MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK*EIIKTTNKKLSKKQQIKTIIDLAKKSNN
IYLATDPDREGEAIS*HVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRSFVKEE*YQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQRV
IDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG*SANKTTLIAQQMFEGLEINNQQLALITYPRTDSERLSD
DFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV*NKTLSALMKVP
TFNKQVIRFINNEYKFYTAYKTIRTLGY*ILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF*KDLVS
TFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLV
KRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ
>Mature_677_residues
MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK*EIIKTTNKKLSKKQQIKTIIDLAKKSNN
IYLATDPDREGEAIS*HVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVK
KNLHGLSAGRVQSIALLFVVERELERRSFVKEE*YQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQRV
IDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG*SANKTTLIAQQMFEGLEINNQQLALITYPRTDSERLSD
DFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV*NKTLSALMKVP
TFNKQVIRFINNEYKFYTAYKTIRTLGY*ILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAA
LKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF*KDLVS
TFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLV
KRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=584, Percent_Identity=24.486301369863, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=21.9903691813804, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1787529, Length=694, Percent_Identity=30.835734870317, Blast_Score=289, Evalue=5e-79,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=24.6835443037975, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI48994931, Length=80, Percent_Identity=38.75, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563869, Length=584, Percent_Identity=22.6027397260274, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=483, Percent_Identity=23.8095238095238, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77330; Mature: 77330

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKEIIKTTNKK
CCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LSKKQQIKTIIDLAKKSNNIYLATDPDREGEAISHVYDLLPKKDKERVKRITFNEITQKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVER
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELERRSFVKEEYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQRVIDD
HHHHHHHHHHHHHHHHEEHHHHHEEHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGSANKTTLIAQQMFEGLEINNQQ
HHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
LALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPV
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCHHHHHHCCCCC
DINLKPEEIKDYVSLDIYKLYNLVNKTLSALMKVPTFNKQVIRFINNEYKFYTAYKTIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHH
LGYILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAALKNAGV
HHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
GRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLD
CCCCHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
VIANGKLFKDLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IACSNFPTCRFTESLEKPEILNELCPNCSKSLVKRKNRKNKYFIGCTGYPECNFIRNLDE
EEECCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCC
SNNDKNDFNQ
CCCCCCCCCC
>Mature Secondary Structure
MQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKEIIKTTNKK
CCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
LSKKQQIKTIIDLAKKSNNIYLATDPDREGEAISHVYDLLPKKDKERVKRITFNEITQKA
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLFVVER
HHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ELERRSFVKEEYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQRVIDD
HHHHHHHHHHHHHHHHEEHHHHHEEHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGSANKTTLIAQQMFEGLEINNQQ
HHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCE
LALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPV
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCHHHHHHCCCCC
DINLKPEEIKDYVSLDIYKLYNLVNKTLSALMKVPTFNKQVIRFINNEYKFYTAYKTIRT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHH
LGYILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAALKNAGV
HHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
GRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLD
CCCCHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHH
VIANGKLFKDLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRF
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IACSNFPTCRFTESLEKPEILNELCPNCSKSLVKRKNRKNKYFIGCTGYPECNFIRNLDE
EEECCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCC
SNNDKNDFNQ
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]