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The map label for this gene is tsf

Identifier: 13358078

GI number: 13358078

Start: 635625

End: 636500

Strand: Reverse

Name: tsf

Synonym: UU514

Alternate gene names: 13358078

Gene position: 636500-635625 (Counterclockwise)

Preceding gene: 13358083

Following gene: 13358077

Centisome position: 84.67

GC content: 29.34

Gene sequence:

>876_bases
ATGACAAAAGCTGAATTAGTTAAAGAATTAAGAATAAGAACACAAGCTAGTATGAGTGAATGTATTAAAGCTTTAGATGC
GTCAGAAAATGATATTGAAAAAGCAATAGTATGATTACGAGAAAATGGTGCTATTAAGGCTGCTAATAAACTAAAAAATG
CAGCGACAGATGGTGTTGTATTGGCCAAAAAAATAAATAATAAAGCAATTCTTATTGAGGTTAATTGTCAAACTGACTTT
GTTGCTAAGAATGAAAATTTTTTAGCTTATGCTAATCAAATTTTAGAAGAAGCATTAGCTAAGGTTGAAAATAAAGAAGA
TTTTGATAAATTAATTATAAATGGAAAACCAATTGCTGAATCAGGATTAGATTTAACAGCATATATTGGTGAAAAAATCG
TTTTTAGACGTGGAGAAATTTTAAAAGCTAATGATGACCAAACTTTAGGTGTTTATACTCACAATAACAACAGAGTTGCT
GCTATTATTTTAGTTGATGGAAAAGTTGAAGATGAAGTTGTTCGAAATGTAGCAATGCATGCTGCAGCAATGCGTCCACG
TTATTTAAATGAAAAAGTTGTTGATAAGTTATGATTAGCGAAAGAACGTGAAATTATTGTTAATCAATTAGAACATGAAG
GTAAACCAGCAGCTTTTGCTGCAAAAATTATTGACGGGCGTTTAAATAAAATTTTAAAAGAAAATTGTTTAGTAGATCAA
TCATATTTTAAACAACCAGAATTAACAATTGAAAAATATTTAAAGAATAATAACGCTGTTGCAGTAGGTTATTTCTCATA
TGAAGTAGGCGAAGGAATTGAAAAAGCTCCTCAAATGAGTTTTGCTGATGAAGTAGCAGCACAAATGAAAAAATAA

Upstream 100 bases:

>100_bases
TTTTTATTCATTTAATCAATATATATTATGAAAACAATAATAAATTATATATAATATATAAGATATGCTAAAAACATGTA
TTTAATAAAGGTGAATATAA

Downstream 100 bases:

>100_bases
TTTTTAAATGTAAAAATAGAAGGAAACAAAGTTTCTTTCTATTTTTATAGTGTAGTCAATAAGGAATATGAAAGGAAATT
CATATGCGAAAACAACGAAT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTKAELVKELRIRTQASMSECIKALDASENDIEKAIVWLRENGAIKAANKLKNAATDGVVLAKKINNKAILIEVNCQTDF
VAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVA
AIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKLWLAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQ
SYFKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK

Sequences:

>Translated_291_residues
MTKAELVKELRIRTQASMSECIKALDASENDIEKAIV*LRENGAIKAANKLKNAATDGVVLAKKINNKAILIEVNCQTDF
VAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVA
AIILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKL*LAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQ
SYFKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK
>Mature_290_residues
TKAELVKELRIRTQASMSECIKALDASENDIEKAIV*LRENGAIKAANKLKNAATDGVVLAKKINNKAILIEVNCQTDFV
AKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAESGLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAA
IILVDGKVEDEVVRNVAMHAAAMRPRYLNEKVVDKL*LAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQS
YFKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Escherichia coli, GI1786366, Length=289, Percent_Identity=40.1384083044983, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32127; Mature: 31996

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAELVKELRIRTQASMSECIKALDASENDIEKAIVLRENGAIKAANKLKNAATDGVVL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCEEHHHHHHHCCCCCEEE
AKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAES
EEECCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCC
GLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHA
CCEEEEHHCCHHEEECCCEEECCCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHH
AAMRPRYLNEKVVDKLLAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQSY
HHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
FKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK
HCCCCHHHHHHHCCCCEEEEEEEEEHHCCCHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TKAELVKELRIRTQASMSECIKALDASENDIEKAIVLRENGAIKAANKLKNAATDGVVL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCEEHHHHHHHCCCCCEEE
AKKINNKAILIEVNCQTDFVAKNENFLAYANQILEEALAKVENKEDFDKLIINGKPIAES
EEECCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCC
GLDLTAYIGEKIVFRRGEILKANDDQTLGVYTHNNNRVAAIILVDGKVEDEVVRNVAMHA
CCEEEEHHCCHHEEECCCEEECCCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHH
AAMRPRYLNEKVVDKLLAKEREIIVNQLEHEGKPAAFAAKIIDGRLNKILKENCLVDQSY
HHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
FKQPELTIEKYLKNNNAVAVGYFSYEVGEGIEKAPQMSFADEVAAQMKK
HCCCCHHHHHHHCCCCEEEEEEEEEHHCCCHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA