Click here to switch to the map view.

The map label for this gene is def

Identifier: 13358028

GI number: 13358028

Start: 528910

End: 529506

Strand: Direct

Name: def

Synonym: UU465

Alternate gene names: 13358028

Gene position: 528910-529506 (Clockwise)

Preceding gene: 13358019

Following gene: 13358029

Centisome position: 70.36

GC content: 28.14

Gene sequence:

>597_bases
ATGTACAATATTAAATTTTTAGATTTATTAAATTCAAATTTAAAGCCTAACCCTCAATGAATTTTTAAAGATCCACATCC
CATTTTACGCGAAGTAACTCAAGATATAGAAGGTAATGAATTATCAAAAGATGATATTTATTATCTTAAAAAAATGGTCC
GTTATATTGATGTTTGTTATCATAATCAAGCCAAAAAATATAAAATTCGTTCAGGAATTGCGATCGCTGCAAATCAAGTT
GGTTGAAACAAACGCGCAACTTATATCCATTTTAATGATGAAGCCAAAGAACATCATTATTTATTAATTAATCCTCATAT
TATTAAACGTTCATCTGAAATAGCTTATTTAAATCCAGGTGAAGGATGTTTATCTGTTGATGATGATCGTTCTGGATATG
TTATTAGAAATAAAAAAGTTCATGTTAAAGCATATGATCTAATTAGCGAACAATTTATTGATCAAGAATTTAGTGGTATT
ATTGCAATTTGCATCCAACATGAAATTGGTCATTTAGATGCTGGTTTATATTATGACAATATCAATCAACAGCAACCATT
TTATGCCGATCCTAGTTGAACAAAGATAGGAAGATAA

Upstream 100 bases:

>100_bases
TTATAAACTTTTATATCATTTATAATTATTATTAAAACATGGTTAATAAAATAAAAACCAATTAGTTATGATTTCATTTC
AAAAAATAGGAAGTAAGTAA

Downstream 100 bases:

>100_bases
AATCTTATGGCAAATAAATACGATGGGAATGCTATTAAAATCTTAGAAGGGTTGGAAGCTGTTCGTAAGCGTCCTGGAAT
GTATATTGGCTCTACAAGTA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MYNIKFLDLLNSNLKPNPQWIFKDPHPILREVTQDIEGNELSKDDIYYLKKMVRYIDVCYHNQAKKYKIRSGIAIAANQV
GWNKRATYIHFNDEAKEHHYLLINPHIIKRSSEIAYLNPGEGCLSVDDDRSGYVIRNKKVHVKAYDLISEQFIDQEFSGI
IAICIQHEIGHLDAGLYYDNINQQQPFYADPSWTKIGR

Sequences:

>Translated_198_residues
MYNIKFLDLLNSNLKPNPQ*IFKDPHPILREVTQDIEGNELSKDDIYYLKKMVRYIDVCYHNQAKKYKIRSGIAIAANQV
G*NKRATYIHFNDEAKEHHYLLINPHIIKRSSEIAYLNPGEGCLSVDDDRSGYVIRNKKVHVKAYDLISEQFIDQEFSGI
IAICIQHEIGHLDAGLYYDNINQQQPFYADPS*TKIGR
>Mature_198_residues
MYNIKFLDLLNSNLKPNPQ*IFKDPHPILREVTQDIEGNELSKDDIYYLKKMVRYIDVCYHNQAKKYKIRSGIAIAANQV
G*NKRATYIHFNDEAKEHHYLLINPHIIKRSSEIAYLNPGEGCLSVDDDRSGYVIRNKKVHVKAYDLISEQFIDQEFSGI
IAICIQHEIGHLDAGLYYDNINQQQPFYADPS*TKIGR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=108, Percent_Identity=36.1111111111111, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22531; Mature: 22531

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNIKFLDLLNSNLKPNPQIFKDPHPILREVTQDIEGNELSKDDIYYLKKMVRYIDVCYH
CCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
NQAKKYKIRSGIAIAANQVGNKRATYIHFNDEAKEHHYLLINPHIIKRSSEIAYLNPGEG
CCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCEEEECCCCC
CLSVDDDRSGYVIRNKKVHVKAYDLISEQFIDQEFSGIIAICIQHEIGHLDAGLYYDNIN
CEEECCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEECCCC
QQQPFYADPSTKIGR
CCCCEECCCCCCCCC
>Mature Secondary Structure
MYNIKFLDLLNSNLKPNPQIFKDPHPILREVTQDIEGNELSKDDIYYLKKMVRYIDVCYH
CCCEEEEHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
NQAKKYKIRSGIAIAANQVGNKRATYIHFNDEAKEHHYLLINPHIIKRSSEIAYLNPGEG
CCCCEEEECCCEEEEECCCCCCEEEEEEECCCCCCCEEEEECCCEEECCCCEEEECCCCC
CLSVDDDRSGYVIRNKKVHVKAYDLISEQFIDQEFSGIIAICIQHEIGHLDAGLYYDNIN
CEEECCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEECCCC
QQQPFYADPSTKIGR
CCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA