Click here to switch to the map view.

The map label for this gene is parE/gyrB

Identifier: 13358029

GI number: 13358029

Start: 529513

End: 531441

Strand: Direct

Name: parE/gyrB

Synonym: UU466

Alternate gene names: 13358029

Gene position: 529513-531441 (Clockwise)

Preceding gene: 13358028

Following gene: 13358030

Centisome position: 70.44

GC content: 28.36

Gene sequence:

>1929_bases
ATGGCAAATAAATACGATGGGAATGCTATTAAAATCTTAGAAGGGTTGGAAGCTGTTCGTAAGCGTCCTGGAATGTATAT
TGGCTCTACAAGTAGCACTGGATTACATCATTTAATTTGAGAAATTGTAGATAATTCAATTGATGAAGTTATGAATGCTA
ACGCAAAAAACATTAATGTTATTTTGCATGAAGATAATTCAATTAGTGTTTTAGATGATGGGCGTGGTATACCAGTTGAC
ATTAATTCTCAAACAAAAATTTCTACTGTTGAAACTGTTCTAACAGTTTTGCATGCTGGAGGTAAGTTTGATGAAAGTGC
ATACAAAACTGCTGGTGGTTTACATGGTGTTGGTTCATCAGTAGTTAATGCTTTAAGTTCTTGATTAATTTGTGAAGTTT
ATCGTGATCAAAAAATTTACCAAGCGAAATTTATTAATGGAGGTCATATTAATCAATCTTTAAAAGTAATTGGAACAACT
AAAAAAACAGGAACATTAATTCATTTCTTACCCGATCCTCTAATTTTTAAAAATTTAGTTTTTAATCCTAACATAATAAA
AGAGCGTCTACATGAATCAACTTTTTTAATAAAAGATTTAAAAATTATTTTTGAAGATAAAATTAATAAAAAAAAGTACG
AATTTATTAATAATCAAGGTTTAATTGATTTTATTAAATTTATTAATGAAGCTAAAAAGACCTTTTCTGATGTTATTTAT
TTTAGAAGTAATATTAACAAAATAGATGTTGAAATTGCTTTTCAATATAGTGATCAAAATAATGAAATTATGGTTTCATT
TGCCAATTCTGTGAAAACTAGTGAAGGTGGTGTACACGAAAATGCTTTTAAAAACGCTTTAACAAGTGTTGTTAATAATT
ATGCAAGAAAACATAATTTACTTAAAGAAAAAGACAAAAATTTAGAAGGTGATGATATACGTGAGGGTTTATCAAGTGTA
ATTTCACTTCGAATCCCCGAAAGCTTAATTAGCTATGAGGGTCAAACTAAAAACAAATTATTCACACCAGAAGCAAATGA
AGCAGTAAAAAAAACAATTGAAGATAATTTTAGTTTTTGATTAGAAGAAAATAAAACACAAGCTTTAGATTTAGTTAATC
GAGCAATTCTTGCACGTGATGCTAAATTGGCTGCAAAGCGTGCTCGTGAAGAAACTAAAAAAGTTAAAAAAATTAAAGAA
GAGCGAGGAATGGGCGGAAAATTAACGCCAGCACAAAGTAAAGATCCAGTTTTAAATGAATTATTTTTGGTTGAAGGTGA
TTCAGCTGGTGGATCAGCTAAACTAGGACGAAATAAAAAATACCAGGCTATTTTGCCCTTAAGAGGTAAGGTTCTTAATG
TTTTAAAAGCTCGTTTAGTTGATGTTTTAAAAAATGAAGAAATAGCATCAATCTTTACTTGTTTAGGAACAGGAATTGGT
GCAGAATTTGATTTAAAAAAATTAAAATATCATAAAATTATTATTATGACCGATGCAGATACCGATGGTAGTCACATCCA
AGTTTTATTATTAACTTTATTTTATCGTTTTATGCGACCTTTAATTGAAAATGGTAATATTTACATAGCTTTACCTCCGC
TTTATAAATTAACAAATAAAAATAACAAAAAATTCTTTTATGCTTGGGATGATGTTGAATTAGATCAACTAAAAAAAGAG
CAAAAAAATTATGAAATCCAACGTTATAAAGGTCTTGGCGAAATGAACGCTGATCAATTATTTGAAACAACAATGGATCC
AAACAATCGTTTATTATTAAAAATTAATATTAATGACATTTTACAAGCTGAACGGCAAATCAATACTTTAATGGGTAATG
ACGTAAGTATTCGAAAGCAGTGAATTGATAATAATATTGATTTTAGTGTCATTGACGAATTACAAATCAATAATGAGGAG
TCTAAATAA

Upstream 100 bases:

>100_bases
AATTGGTCATTTAGATGCTGGTTTATATTATGACAATATCAATCAACAGCAACCATTTTATGCCGATCCTAGTTGAACAA
AGATAGGAAGATAAAATCTT

Downstream 100 bases:

>100_bases
AAAATATGAGTGTAAATCATCAAAAAATTATTAATACACCTTTAGACAACATTGTTGGTGAAAGTTATGCAAAATATGCA
AAATACATTATTCAAGATCG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B; TopIIB [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLIWEIVDNSIDEVMNANAKNINVILHEDNSISVLDDGRGIPVD
INSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSVVNALSSWLICEVYRDQKIYQAKFINGGHINQSLKVIGTT
KKTGTLIHFLPDPLIFKNLVFNPNIIKERLHESTFLIKDLKIIFEDKINKKKYEFINNQGLIDFIKFINEAKKTFSDVIY
FRSNINKIDVEIAFQYSDQNNEIMVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLKEKDKNLEGDDIREGLSSV
ISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSFWLEENKTQALDLVNRAILARDAKLAAKRAREETKKVKKIKE
ERGMGGKLTPAQSKDPVLNELFLVEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIG
AEFDLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNKKFFYAWDDVELDQLKKE
QKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQAERQINTLMGNDVSIRKQWIDNNIDFSVIDELQINNEE
SK

Sequences:

>Translated_642_residues
MANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLI*EIVDNSIDEVMNANAKNINVILHEDNSISVLDDGRGIPVD
INSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSVVNALSS*LICEVYRDQKIYQAKFINGGHINQSLKVIGTT
KKTGTLIHFLPDPLIFKNLVFNPNIIKERLHESTFLIKDLKIIFEDKINKKKYEFINNQGLIDFIKFINEAKKTFSDVIY
FRSNINKIDVEIAFQYSDQNNEIMVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLKEKDKNLEGDDIREGLSSV
ISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSF*LEENKTQALDLVNRAILARDAKLAAKRAREETKKVKKIKE
ERGMGGKLTPAQSKDPVLNELFLVEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIG
AEFDLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNKKFFYAWDDVELDQLKKE
QKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQAERQINTLMGNDVSIRKQ*IDNNIDFSVIDELQINNEE
SK
>Mature_641_residues
ANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLI*EIVDNSIDEVMNANAKNINVILHEDNSISVLDDGRGIPVDI
NSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSVVNALSS*LICEVYRDQKIYQAKFINGGHINQSLKVIGTTK
KTGTLIHFLPDPLIFKNLVFNPNIIKERLHESTFLIKDLKIIFEDKINKKKYEFINNQGLIDFIKFINEAKKTFSDVIYF
RSNINKIDVEIAFQYSDQNNEIMVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLKEKDKNLEGDDIREGLSSVI
SLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSF*LEENKTQALDLVNRAILARDAKLAAKRAREETKKVKKIKEE
RGMGGKLTPAQSKDPVLNELFLVEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIGA
EFDLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNKKFFYAWDDVELDQLKKEQ
KNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQAERQINTLMGNDVSIRKQ*IDNNIDFSVIDELQINNEES
K

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=637, Percent_Identity=25.1177394034537, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI19913406, Length=608, Percent_Identity=23.3552631578947, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI48994957, Length=563, Percent_Identity=46.1811722912966, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI1789408, Length=609, Percent_Identity=38.0952380952381, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI212645845, Length=562, Percent_Identity=26.3345195729537, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535065, Length=650, Percent_Identity=23.2307692307692, Blast_Score=115, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=179, Percent_Identity=29.608938547486, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=625, Percent_Identity=25.44, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17136538, Length=638, Percent_Identity=26.0188087774295, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: 5.99.1.-

Molecular weight: Translated: 71834; Mature: 71703

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLIEIVDNSIDEVMNANAKNINVI
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LHEDNSISVLDDGRGIPVDINSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSV
EECCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHH
VNALSSLICEVYRDQKIYQAKFINGGHINQSLKVIGTTKKTGTLIHFLPDPLIFKNLVFN
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCEEEECCCHHHHHHHCCC
PNIIKERLHESTFLIKDLKIIFEDKINKKKYEFINNQGLIDFIKFINEAKKTFSDVIYFR
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SNINKIDVEIAFQYSDQNNEIMVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLK
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKDKNLEGDDIREGLSSVISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSFLEE
HHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHC
NKTQALDLVNRAILARDAKLAAKRAREETKKVKKIKEERGMGGKLTPAQSKDPVLNELFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
VEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIGAEF
EECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
DLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNK
CHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCCC
KFFYAWDDVELDQLKKEQKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQA
EEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHH
ERQINTLMGNDVSIRKQIDNNIDFSVIDELQINNEESK
HHHHHHHHCCCCCHHHHHCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
ANKYDGNAIKILEGLEAVRKRPGMYIGSTSSTGLHHLIEIVDNSIDEVMNANAKNINVI
CCCCCCCHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LHEDNSISVLDDGRGIPVDINSQTKISTVETVLTVLHAGGKFDESAYKTAGGLHGVGSSV
EECCCCEEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHH
VNALSSLICEVYRDQKIYQAKFINGGHINQSLKVIGTTKKTGTLIHFLPDPLIFKNLVFN
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCEEEECCCHHHHHHHCCC
PNIIKERLHESTFLIKDLKIIFEDKINKKKYEFINNQGLIDFIKFINEAKKTFSDVIYFR
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SNINKIDVEIAFQYSDQNNEIMVSFANSVKTSEGGVHENAFKNALTSVVNNYARKHNLLK
CCCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKDKNLEGDDIREGLSSVISLRIPESLISYEGQTKNKLFTPEANEAVKKTIEDNFSFLEE
HHCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHC
NKTQALDLVNRAILARDAKLAAKRAREETKKVKKIKEERGMGGKLTPAQSKDPVLNELFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEE
VEGDSAGGSAKLGRNKKYQAILPLRGKVLNVLKARLVDVLKNEEIASIFTCLGTGIGAEF
EECCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
DLKKLKYHKIIIMTDADTDGSHIQVLLLTLFYRFMRPLIENGNIYIALPPLYKLTNKNNK
CHHEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHEECCCCCC
KFFYAWDDVELDQLKKEQKNYEIQRYKGLGEMNADQLFETTMDPNNRLLLKININDILQA
EEEEEECCCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHCCCCCCEEEEEECHHHHHHH
ERQINTLMGNDVSIRKQIDNNIDFSVIDELQINNEESK
HHHHHHHHCCCCCHHHHHCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7783737; 12949158 [H]