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The map label for this gene is truB

Identifier: 13357914

GI number: 13357914

Start: 401965

End: 402648

Strand: Direct

Name: truB

Synonym: UU354

Alternate gene names: 13357914

Gene position: 401965-402648 (Clockwise)

Preceding gene: 13357911

Following gene: 13357915

Centisome position: 53.47

GC content: 26.61

Gene sequence:

>684_bases
ATGAGTGCTATTAGTAAAAATATTATTATCATTAACAAACCAATAAAATGAACTTCTAATGATGTTGTTCAAAAGGTTAA
ACGGGTTATTGGTGCTAAAAAAGTTGGACATGCTGGAACTTTAGATCCTAATGCTTCAGGTGTTTTAGTATTGGGAATTA
ATGAAGGAACAAAACTATTAACAAAATTAATACTAGATAATAAATCATATATTGCTGAAATTAAATTTGGAACATCAACA
AATACTTATGATGCAGCAGGTGAAATTGTTTCATCTACAAACCGAATGGTTACATTAACTGAGGTTACTAAAATAGTTAA
AGATTTTTATAAAAATGATTATTGACAAAAACCCCCACAATTTAGCGCTTTAAAAATCAACGGGCAAAAAGCTTATGTTT
TAGCACGTCAAAAAGTTGATTTTGAAATAGCACCACGATTAGTTAAAATATTTAAATATCAAATTATGGATTTCAATTAT
GAAAAACAAATTTTAAAAATTTCTTTACATGTTTCTAAAGGTTTTTATGTTCGTTCTTTTGCTGTTGATTTAGCTACTAA
AATTAATAATCTAGCTCATTTATTGACATTAATAAGGACGCAAAGTGGACCTTTTGAGATTAAAGATGCAATTGAAATTG
AACAAGTCTATGATTATTGAAATAACATCAACAAATATTTATAA

Upstream 100 bases:

>100_bases
ATTTTCCTTTATCTTAATTAATACTATATAAACTTTATTTATTAATATTAACAAATTATTATTAATAATTTATTCTTATT
ACTGATAAAATAATAACATT

Downstream 100 bases:

>100_bases
AATTAAGCAACAATTCGTCATTAATGAATTAATTATTGGTTTTTTTGATGGAATTCATTTAGGTCATATGAATTTATTAT
CAGATCCTAATAACCAAACT

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSAISKNIIIINKPIKWTSNDVVQKVKRVIGAKKVGHAGTLDPNASGVLVLGINEGTKLLTKLILDNKSYIAEIKFGTST
NTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDYWQKPPQFSALKINGQKAYVLARQKVDFEIAPRLVKIFKYQIMDFNY
EKQILKISLHVSKGFYVRSFAVDLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDYWNNINKYL

Sequences:

>Translated_227_residues
MSAISKNIIIINKPIK*TSNDVVQKVKRVIGAKKVGHAGTLDPNASGVLVLGINEGTKLLTKLILDNKSYIAEIKFGTST
NTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDY*QKPPQFSALKINGQKAYVLARQKVDFEIAPRLVKIFKYQIMDFNY
EKQILKISLHVSKGFYVRSFAVDLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDY*NNINKYL
>Mature_226_residues
SAISKNIIIINKPIK*TSNDVVQKVKRVIGAKKVGHAGTLDPNASGVLVLGINEGTKLLTKLILDNKSYIAEIKFGTSTN
TYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDY*QKPPQFSALKINGQKAYVLARQKVDFEIAPRLVKIFKYQIMDFNYE
KQILKISLHVSKGFYVRSFAVDLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDY*NNINKYL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=194, Percent_Identity=32.4742268041237, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI2367200, Length=213, Percent_Identity=32.3943661971831, Blast_Score=117, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324037, Length=142, Percent_Identity=36.6197183098592, Blast_Score=79, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323204, Length=231, Percent_Identity=23.8095238095238, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25153; Mature: 25021

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAISKNIIIINKPIKTSNDVVQKVKRVIGAKKVGHAGTLDPNASGVLVLGINEGTKLLT
CCCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEEECCCHHHHH
KLILDNKSYIAEIKFGTSTNTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDYQKPPQFS
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCE
ALKINGQKAYVLARQKVDFEIAPRLVKIFKYQIMDFNYEKQILKISLHVSKGFYVRSFAV
EEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEHHHHH
DLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDYNNINKYL
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAISKNIIIINKPIKTSNDVVQKVKRVIGAKKVGHAGTLDPNASGVLVLGINEGTKLLT
CCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHCCCCCCCCCCCCEEEEEEECCCHHHHH
KLILDNKSYIAEIKFGTSTNTYDAAGEIVSSTNRMVTLTEVTKIVKDFYKNDYQKPPQFS
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCE
ALKINGQKAYVLARQKVDFEIAPRLVKIFKYQIMDFNYEKQILKISLHVSKGFYVRSFAV
EEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCEEEHHHHH
DLATKINNLAHLLTLIRTQSGPFEIKDAIEIEQVYDYNNINKYL
HHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA