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The map label for this gene is ribF

Identifier: 13357915

GI number: 13357915

Start: 402614

End: 403465

Strand: Direct

Name: ribF

Synonym: UU355

Alternate gene names: 13357915

Gene position: 402614-403465 (Clockwise)

Preceding gene: 13357914

Following gene: 13357916

Centisome position: 53.56

GC content: 21.83

Gene sequence:

>852_bases
ATGATTATTGAAATAACATCAACAAATATTTATAAAATTAAGCAACAATTCGTCATTAATGAATTAATTATTGGTTTTTT
TGATGGAATTCATTTAGGTCATATGAATTTATTATCAGATCCTAATAACCAAACTATTTTAACTTTTAAAAATATCCCAC
GTAAAATAAAAAAATTATATGATTTTAATGAGCGAATTCAACAATTAGAAGATTTAGGATTTAAAAGGATTTTTATTTAT
GATATTGATCAAAACAATTTAAGTGGGGAAGAATTTATTGATCAAATTTTAAAGCCACTAACTCCAAAAAAAATTATTGT
TGGTGCTAATTTTACTTATGGCAATAATTTTTGTAATGCATCTAGTCTAAAACAATATTTTAATGTTGAAATTAAAATTA
TTACTAATGATGTTAGTACAACTAAAATTAAAGAATTAATTATAAATAAACAGGTTGAAATAGCTAATAAATTATTAATT
AAACCATATTATCGTGTGGGAAATGTTGTACGTGGCGATCAAATAGCTCGTAATATTGGTTTTAATACTGCTAATATTTT
ATGTGATAATAATTTAATTGATATTGCTGAAGGTGTTTACAAAGCACAAGTAATTTTTAATAATAAAAAGTATGATAGTG
TAGTTTATTTAGGAATCCCTAAAACAATTAATACTCGCTCTTTTTCAATGATTGAAGCACATATTTTAGACTTTAATCAA
AATATTTATGACGAAAGAATTAAGATTGTTTTTTTGAAATATTTAGCTCCTAATTTAAAATTTAATAATATAGATGAATT
AATCACTGCAATTAAAAATTATATTAAATTGGTTCTTGATAAAACAAATTAG

Upstream 100 bases:

>100_bases
TTAGCTACTAAAATTAATAATCTAGCTCATTTATTGACATTAATAAGGACGCAAAGTGGACCTTTTGAGATTAAAGATGC
AATTGAAATTGAACAAGTCT

Downstream 100 bases:

>100_bases
GTATATAAACCAATAAATAATTTTTTATAGTAGTAAAATCTTAATGGCTATTATAAAAAAAACATAAACTTTCATGGTTT
ATTTTTTGTTTAAAGTAGTT

Product: riboflavin kinase/FAD synthetase

Products: NA

Alternate protein names: FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase; Riboflavin kinase; Flavokinase; tRNA-specific 2-thiouridylase mnmA [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLYDFNERIQQLEDLGFKRIFIY
DIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNASSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLI
KPYYRVGNVVRGDQIARNIGFNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQ
NIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN

Sequences:

>Translated_283_residues
MIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLYDFNERIQQLEDLGFKRIFIY
DIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNASSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLI
KPYYRVGNVVRGDQIARNIGFNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQ
NIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN
>Mature_283_residues
MIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLYDFNERIQQLEDLGFKRIFIY
DIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNASSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLI
KPYYRVGNVVRGDQIARNIGFNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQ
NIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN

Specific function: Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 [H]

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the mnmA/TRMU family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=295, Percent_Identity=25.7627118644068, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729
- InterPro:   IPR004506 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase; PF03054 tRNA_Me_trans [H]

EC number: =2.7.7.2; =2.7.1.26 [H]

Molecular weight: Translated: 32770; Mature: 32770

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLY
CEEEEECCCHHHHHHHHHHHHHHHHHHCCEEECCCHHCCCCCCCEEEEEHHHHHHHHHHH
DFNERIQQLEDLGFKRIFIYDIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNA
HHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCEECCCCCCCH
SSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLIKPYYRVGNVVRGDQIARNIG
HHHHHHHCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHCCEECHHHHHHHCC
FNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQ
CCCEEEEECCCHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHEEEEECCCC
NIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN
CHHHHHHHEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MIIEITSTNIYKIKQQFVINELIIGFFDGIHLGHMNLLSDPNNQTILTFKNIPRKIKKLY
CEEEEECCCHHHHHHHHHHHHHHHHHHCCEEECCCHHCCCCCCCEEEEEHHHHHHHHHHH
DFNERIQQLEDLGFKRIFIYDIDQNNLSGEEFIDQILKPLTPKKIIVGANFTYGNNFCNA
HHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCEECCCCCCCH
SSLKQYFNVEIKIITNDVSTTKIKELIINKQVEIANKLLIKPYYRVGNVVRGDQIARNIG
HHHHHHHCEEEEEEECCCCHHHHHHHHHCCHHHHHHHHHCCCHHHHCCEECHHHHHHHCC
FNTANILCDNNLIDIAEGVYKAQVIFNNKKYDSVVYLGIPKTINTRSFSMIEAHILDFNQ
CCCEEEEECCCHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCCCCCHHHHEEEEECCCC
NIYDERIKIVFLKYLAPNLKFNNIDELITAIKNYIKLVLDKTN
CHHHHHHHEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949158 [H]