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The map label for this gene is leuB [H]

Identifier: 126698576

GI number: 126698576

Start: 1156470

End: 1157540

Strand: Direct

Name: leuB [H]

Synonym: CD0992

Alternate gene names: 126698576

Gene position: 1156470-1157540 (Clockwise)

Preceding gene: 126698575

Following gene: 126698577

Centisome position: 26.96

GC content: 32.96

Gene sequence:

>1071_bases
ATGAATTGTAATATAGCAGTCATAAAAGGTGATGGTGTTGGACCGGAAATTATAGATGAAGGTATAAAGGTTCTAAATAA
AATTTGTTGTAAATTTAATCATAGATTCGATTGTGAATATGTATTAGCTGGAGGATGCGCGATTGATGAAACTGGAGAAC
CTCTACCTAATAAAACTGTTGAAATTTGTCGAAAAAACGAAGCTGTATTGCTTGGTGCTGTTGGAGGACCAAAGTGGGAT
AAATGTAAGGGAGATAAAAGACCAGAGTCTGGTCTTTTAAAATTAAGAGAGTCATTGGGTTTATTTGCAAATTTAAGACC
AGCTACAATGTATGAAAGTATAAAAGAAGCTTCACCACTTAGAACGGATATAGTTGAGAAAGGCATTGATTTTGTTGTTG
TAAGAGAATTAACAGGGGGCATTTATTTTGGAGAACGTGGAAGAAAAATCATTGATGGTATCGAAAATGCTTATGATGTA
GAAATATATAACGAAAATGAAATAAGAAGAATTGGTAAGAGAGCTTTTGAAATAGCCAGAAATAGAAATAAAAAACTTAT
AAGTGTAGATAAGGCAAATGTGCTTGAGAGTTCAAGACTTTGGAGAAGTATAATGGAAGACTTAGCTAAAGAGTTTGAAG
ATGTAGAACTGTCACATATGTATGTTGATAATGCTGCTATGCAAGTTGTAAAAGACCCTAGCCAATTTGATGTAATAGTG
ACTAATAATATATTTGGAGATATAATATCTGATGAAGCATCTATGATTACGGGTTCTATAGGTATGTTACCATCAGCATC
ACTTAGAGAAGATAGTTTTGGTATGTATGAACCAATACATGGAAGTGCACCTGATATAGCTGGAAAAGATATTGTAAATC
CAATTGCGACTATTCTATCTGTAAGCATGATGTTAAGACATAGTTTTAACTTAGAAGAAGAAGCTAAGTGTATTGAAGAT
GCTGTACAAAGTGTACTCAATAAAGGTTATAGAACTATTGATATCTATAATGGTGTGGGAAATGTTGTAGGAACAAGAGC
AATGGGCGAATTAATAGTAAATGAAATTTAG

Upstream 100 bases:

>100_bases
AAAAGAATATAAAGCAGAACCATTTCCTGAGTTTATGCAAAATATTATATTAAATGAAGGCTTAATTAACTCAATAAAAG
CAAATAGGGGGTAGTGGAGT

Downstream 100 bases:

>100_bases
AATTATAGTTTAATTTGAATAATATAATTTAAATATATAACTTGTAGAATTAGTAAAAATCTATTGTAGTATAATTTGTT
TTATGAGGGGTGTCTCAAAA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAGGCAIDETGEPLPNKTVEICRKNEAVLLGAVGGPKWD
KCKGDKRPESGLLKLRESLGLFANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELSHMYVDNAAMQVVKDPSQFDVIV
TNNIFGDIISDEASMITGSIGMLPSASLREDSFGMYEPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIED
AVQSVLNKGYRTIDIYNGVGNVVGTRAMGELIVNEI

Sequences:

>Translated_356_residues
MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAGGCAIDETGEPLPNKTVEICRKNEAVLLGAVGGPKWD
KCKGDKRPESGLLKLRESLGLFANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELSHMYVDNAAMQVVKDPSQFDVIV
TNNIFGDIISDEASMITGSIGMLPSASLREDSFGMYEPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIED
AVQSVLNKGYRTIDIYNGVGNVVGTRAMGELIVNEI
>Mature_356_residues
MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAGGCAIDETGEPLPNKTVEICRKNEAVLLGAVGGPKWD
KCKGDKRPESGLLKLRESLGLFANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELSHMYVDNAAMQVVKDPSQFDVIV
TNNIFGDIISDEASMITGSIGMLPSASLREDSFGMYEPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIED
AVQSVLNKGYRTIDIYNGVGNVVGTRAMGELIVNEI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=33.6363636363636, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI28178816, Length=326, Percent_Identity=29.1411042944785, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178821, Length=326, Percent_Identity=29.1411042944785, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=29.4985250737463, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178838, Length=330, Percent_Identity=28.7878787878788, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI28178819, Length=166, Percent_Identity=34.3373493975904, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=52.3809523809524, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788101, Length=343, Percent_Identity=34.6938775510204, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1787381, Length=381, Percent_Identity=25.9842519685039, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=30.9248554913295, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=31.3609467455621, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=28.698224852071, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=28.955223880597, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=50.6887052341598, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6322097, Length=342, Percent_Identity=35.672514619883, Blast_Score=162, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=31.3218390804598, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=30.3571428571429, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24643270, Length=355, Percent_Identity=32.112676056338, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24643268, Length=355, Percent_Identity=32.112676056338, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=29.2397660818713, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281362242, Length=337, Percent_Identity=29.673590504451, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648872, Length=337, Percent_Identity=29.673590504451, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=247, Percent_Identity=31.5789473684211, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078635, Length=247, Percent_Identity=31.5789473684211, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078633, Length=247, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24650122, Length=247, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161078637, Length=247, Percent_Identity=31.5789473684211, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=336, Percent_Identity=22.6190476190476, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39370; Mature: 39370

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAGGCAIDETGEPLPNKTV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEECCCCCCCCCHHH
EICRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLFANLRPATMYESIKEASPL
HHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCH
RTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDVEIYNENEIRRIGKRAFEIAR
HHHHHHCCCCEEEEEEHHCCEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
NRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELSHMYVDNAAMQVVKDPSQFDVIV
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCCCEEEEE
TNNIFGDIISDEASMITGSIGMLPSASLREDSFGMYEPIHGSAPDIAGKDIVNPIATILS
ECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTIDIYNGVGNVVGTRAMGELIVNEI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAGGCAIDETGEPLPNKTV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEECCCCCCCCCHHH
EICRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLFANLRPATMYESIKEASPL
HHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCH
RTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDVEIYNENEIRRIGKRAFEIAR
HHHHHHCCCCEEEEEEHHCCEEECCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHH
NRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELSHMYVDNAAMQVVKDPSQFDVIV
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHHHHHCCCCCEEEEE
TNNIFGDIISDEASMITGSIGMLPSASLREDSFGMYEPIHGSAPDIAGKDIVNPIATILS
ECCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTIDIYNGVGNVVGTRAMGELIVNEI
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]