Click here to switch to the map view.

The map label for this gene is rpoD4 [H]

Identifier: 126696812

GI number: 126696812

Start: 1242742

End: 1243683

Strand: Reverse

Name: rpoD4 [H]

Synonym: P9301_14741

Alternate gene names: 126696812

Gene position: 1243683-1242742 (Counterclockwise)

Preceding gene: 126696813

Following gene: 126696810

Centisome position: 75.75

GC content: 35.67

Gene sequence:

>942_bases
ATGATTTCATCATTACCAACACAAGCAGAACCGCCTAAAAGGAGAAGCAATGATCCTATTAGCTGGTATTTGCAAAATAT
CGGTAGAGTTCCTTTATTAACACCTGCCGAGGAGATTGAGTTGGGTAATCAAGTCCAGAAGATGATGATTCTTACAGAAG
ATGGACAATTAAATGAAAAGACTAATGAATTTACAACTCAGCAAAAAAGAACAATAAAAATTGGTCGAAGAGCTAAAGAA
AGAATGATGAAAGCTAATTTAAGATTAGTTGTCAGTGTTGCAAAAAAATATCAAGGTAAAGGACTGGAACTTCTTGATTT
AGTACAAGAAGGTTCTCTTGGGTTGGAGAGGGCTGTTGAAAAATTTGATCCGACAAGGGGGTATAAGTTTTCTACATACG
CTTTTTGGTGGATTAGACAGAGTATGACAAGAGCAATTGCTTGTCAATCAAGAACAATTCGTTTACCTGTTCACTTAAGT
GAAAGGTTAGCTTCAATTAGAAAAGTTAGTAGAGATTTGGCTCATAAACTTGGTGCTATGCCCAGCAGGATTGAAATTGC
AGAGGCTATGGAAATTGATGTAGAAGAATTGGATTCTGTCTTAAGACAAGCTTTATCGACAAGTAGTTTAGATGCTCCAG
TAAATGGCGATGACGGCAGAAGCTTTTTAGGTGATTTAATTGCTGATAGTAATAATGAAGAACCTTTAGATCAAGTTGAA
CAAAAAATGCATCAAGAGCAACTTGGTAAGTGGTTAAGTCATTTGAGCGAGCAAGAACAACATGTTCTCAAATTAAGATT
TGGGCTTGATGCAAATGAGAGACACACACTTGCTGAAATTGGAAGATTATTAGAAGTTTCCAGAGAAAGAGTAAGACAAG
TTGAACTTAAGGCGCTAAGAAAATTAAGGAATTTAACTAGAAAATTACCTAGCAGTATTTAA

Upstream 100 bases:

>100_bases
TAAGTGTACAAATATTACTTGAAATCTGAAGCGCGCATTAAATATTTGGCTTTTTCATTAAAAGTGTAGGAAAGTTTTAA
TACCTAAATTAGGTCAAAAA

Downstream 100 bases:

>100_bases
TCTAATCTTCTGCCCAAATATATTTGGTTAATTCTTCTGAAGGTGGTTCGGGAGCTTCAATAGCATTTTTAACTGACTCA
GATATCTCTGCATCAATTTT

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MISSLPTQAEPPKRRSNDPISWYLQNIGRVPLLTPAEEIELGNQVQKMMILTEDGQLNEKTNEFTTQQKRTIKIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLASIRKVSRDLAHKLGAMPSRIEIAEAMEIDVEELDSVLRQALSTSSLDAPVNGDDGRSFLGDLIADSNNEEPLDQVE
QKMHQEQLGKWLSHLSEQEQHVLKLRFGLDANERHTLAEIGRLLEVSRERVRQVELKALRKLRNLTRKLPSSI

Sequences:

>Translated_313_residues
MISSLPTQAEPPKRRSNDPISWYLQNIGRVPLLTPAEEIELGNQVQKMMILTEDGQLNEKTNEFTTQQKRTIKIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLASIRKVSRDLAHKLGAMPSRIEIAEAMEIDVEELDSVLRQALSTSSLDAPVNGDDGRSFLGDLIADSNNEEPLDQVE
QKMHQEQLGKWLSHLSEQEQHVLKLRFGLDANERHTLAEIGRLLEVSRERVRQVELKALRKLRNLTRKLPSSI
>Mature_313_residues
MISSLPTQAEPPKRRSNDPISWYLQNIGRVPLLTPAEEIELGNQVQKMMILTEDGQLNEKTNEFTTQQKRTIKIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLASIRKVSRDLAHKLGAMPSRIEIAEAMEIDVEELDSVLRQALSTSSLDAPVNGDDGRSFLGDLIADSNNEEPLDQVE
QKMHQEQLGKWLSHLSEQEQHVLKLRFGLDANERHTLAEIGRLLEVSRERVRQVELKALRKLRNLTRKLPSSI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=259, Percent_Identity=46.3320463320463, Blast_Score=227, Evalue=9e-61,
Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=39.9305555555556, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1789871, Length=247, Percent_Identity=30.3643724696356, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788231, Length=219, Percent_Identity=30.1369863013699, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35798; Mature: 35798

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSLPTQAEPPKRRSNDPISWYLQNIGRVPLLTPAEEIELGNQVQKMMILTEDGQLNEK
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
TNEFTTQQKRTIKIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLASIRKVSRDLAHKLGAM
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
PSRIEIAEAMEIDVEELDSVLRQALSTSSLDAPVNGDDGRSFLGDLIADSNNEEPLDQVE
CCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
QKMHQEQLGKWLSHLSEQEQHVLKLRFGLDANERHTLAEIGRLLEVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRKLPSSI
HHHHHHHHCCCCC
>Mature Secondary Structure
MISSLPTQAEPPKRRSNDPISWYLQNIGRVPLLTPAEEIELGNQVQKMMILTEDGQLNEK
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
TNEFTTQQKRTIKIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLASIRKVSRDLAHKLGAM
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
PSRIEIAEAMEIDVEELDSVLRQALSTSSLDAPVNGDDGRSFLGDLIADSNNEEPLDQVE
CCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
QKMHQEQLGKWLSHLSEQEQHVLKLRFGLDANERHTLAEIGRLLEVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRKLPSSI
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA