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The map label for this gene is pur8 [H]

Identifier: 126447639

GI number: 126447639

Start: 1113068

End: 1114504

Strand: Direct

Name: pur8 [H]

Synonym: BMA10247_A1136

Alternate gene names: 126447639

Gene position: 1113068-1114504 (Clockwise)

Preceding gene: 126447716

Following gene: 126446301

Centisome position: 47.31

GC content: 71.61

Gene sequence:

>1437_bases
ATGACATCCGAGATTTCCGGCAAACGGCGCTGGTATGCGCTCGTGGTTCTGTGTCTGGGCGTGCTGATGATCGTGCTCGA
CACGACCATCGTGAACGTCGCGCTGCCGTCGATCGCGGCCGAGCTGCGTTTTTCCGAGAACGCGCTCGTCTGGGTCGTCA
ACGCCTACATGCTGACGTTCGGCGGCTGCCTGCTGCTCGGCGGCAGGCTCGGCGACCTGTACGGGCACCGGCGCCTCTTC
CTGGCCGGCGTGACGGTGTTCACGGCCGCGTCGCTCGCCTGCGGACTCGCGCAGACGCAGCTCATGCTGATCGCCGCGCG
CGCCGCGCAGGGCATCGGCGGCGCGGTCGTCTCCTCGGTCGCGCTGTCGCTCATCATGACGCTGTTCGTCGAGCCGGGCG
ACTGGGCGAAGGCGATGGGCGTCTACGGCTTCGTCTATGCGGGCGGCGGCAGCCTCGGCGTGCTGCTCGGCGGGCTCCTG
ACGAGCGCGTTGAGCTGGCACTGGGTCTTTCTCGTCAACCTGCCGCTCGGCGCCGTGGTCTATCTGCTGTCGTCGGCGCT
GCTGCCGAAGGCGCGCGCGCAGGCAACGCGCGCGCCGCTCGACATCGCCGGCGCCGTGACCGCCGCGCTGATGTTGGCCG
TCTACGCGGTGGTGAACGGCAACGCGGCCGGCTGGCTGTCCGCGCGCACGCTGAGCTGGCTCGGCGGCGCGGCGGCGCTG
TTCGCCGCGTTCGTCGCGATCGAGGCGCGCGTCGCGCATCCGCTGATGCCGCTCGCGCTCTGCATGAAGCGCAACGTCGC
GATCGCGAACCTGATCGGCATCCTGTGGGCCGCCGCGATGTTCGCGTGGTTCTTCCTCGCCGCGCTGTATCTGCAGCGCG
TGCTCGGCTACGGGCCGTTGCAGATCGGCCTCGCGTTCCTGCCGGCGAACCTGATCATGGGCGTGTTCTCGCTCATCGTC
TCCGCGCTGCTCGTGGTGCGCTTCGGGATCCGCGTGCCGATCGCGGCCGGACTGCTCGCGGCGGCGGCCGGCCTGATGCT
GTTCGCGCGGGCGCCCGTCGGCGGGGACTTCGTCGCCGACGTGCTGCCGGGCATGATCCTGCTGGGCACCGGCGCGGGCA
TCGCGTTCAACCCGGTCCTGCTCGCGGCGATGAGCGGCGTCGATCCGGCGGACTCGGGGCTCGCCTCGGGCATCGTCAAC
ACGTCGTTCATGATGGGCGGCGCGCTCGGCCTCGCGGTGCTCGCGAGCGTCGCGACGGCGCGCACCGATGCGCTGCTCGC
CGCGCGCACGGCCGTGCCCGCCGCGATGGCGGGCGGCTACCATGCGGCGTTCATGCTCGGCGCGGCGTTCGCGATCGTCG
CCGCGCTGATCGGGATGCGGCTGCGGGCCGCCCCGAATCCGGCGGCGATGCAGGGGCTGCAAGGCGCGACGCAGTAG

Upstream 100 bases:

>100_bases
ACCGCGGACGTCGCGTGAGGCCGCGCCGCGCGCCGGCGCCGCGCGGCGTCGGCCGCCGCCGCCGCGTGCGCATCCCCCAT
CACTTCATGGAGTTCCATCG

Downstream 100 bases:

>100_bases
CGCGGTCCCGTCGCGCGGCGAGCTTGGTCGGTGTGTTCGACCGGCGAGTTCGATTGGTGTGTTCGACCGGCAAGTGTGAT
CGACGAATTTGAGCGGCGAG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLF
LAGVTVFTAASLACGLAQTQLMLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDWAKAMGVYGFVYAGGGSLGVLLGGLL
TSALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAAL
FAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSLIV
SALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVN
TSFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ

Sequences:

>Translated_478_residues
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLF
LAGVTVFTAASLACGLAQTQLMLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDWAKAMGVYGFVYAGGGSLGVLLGGLL
TSALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAAL
FAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSLIV
SALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVN
TSFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
>Mature_477_residues
TSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLFL
AGVTVFTAASLACGLAQTQLMLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDWAKAMGVYGFVYAGGGSLGVLLGGLLT
SALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAALF
AAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSLIVS
ALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVNT
SFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=416, Percent_Identity=30.0480769230769, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789042, Length=423, Percent_Identity=26.4775413711584, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788392, Length=358, Percent_Identity=30.7262569832402, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788710, Length=420, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790195, Length=255, Percent_Identity=31.7647058823529, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=391, Percent_Identity=25.5754475703325, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323520, Length=462, Percent_Identity=25.5411255411255, Blast_Score=77, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=30.9210526315789, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=30.0751879699248, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48839; Mature: 48708

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEHHHHHHHHHH
GGCLLLGGRLGDLYGHRRLFLAGVTVFTAASLACGLAQTQLMLIAARAAQGIGGAVVSSV
CHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALSLIMTLFVEPGDWAKAMGVYGFVYAGGGSLGVLLGGLLTSALSWHWVFLVNLPLGAVV
HHHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
YLLSSALLPKARAQATRAPLDIAGAVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAAL
HHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QIGLAFLPANLIMGVFSLIVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVNTSFMMGGALGLAVLASVATA
HHCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEHHHHHHHHHH
GGCLLLGGRLGDLYGHRRLFLAGVTVFTAASLACGLAQTQLMLIAARAAQGIGGAVVSSV
CHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALSLIMTLFVEPGDWAKAMGVYGFVYAGGGSLGVLLGGLLTSALSWHWVFLVNLPLGAVV
HHHHHHHHHCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
YLLSSALLPKARAQATRAPLDIAGAVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAAL
HHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
FAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QIGLAFLPANLIMGVFSLIVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVNTSFMMGGALGLAVLASVATA
HHCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]