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The map label for this gene is pksH [H]

Identifier: 126447145

GI number: 126447145

Start: 1052641

End: 1053411

Strand: Reverse

Name: pksH [H]

Synonym: BMA10247_A1117

Alternate gene names: 126447145

Gene position: 1053411-1052641 (Counterclockwise)

Preceding gene: 126446077

Following gene: 126445871

Centisome position: 44.77

GC content: 66.15

Gene sequence:

>771_bases
ATGCTGAGCCGGCCCGCCGACTACCGGACCATCCGCGTCCGGTACGACGGCGACATCTGCTTCCTGCAGCTTCATCGCCC
CGACGCGCAGAACGCGATCAACAACCGCATGATCGCGGAGTGCATGGACGTGCTCGATCGCTGCGAGCACGCGGCGAAGA
TCGTCGTGTTGCAAGGGCTGCCCGACGTGTTCTGCTTCGGCGCGGACTTCTCCGACATTGCCGAAAAGCCGGATGCGCTG
ATCGATTCCGATGCGATCTACGGCTTATGGCACCGGCTCGCGACGGGGCCGTACATCACGATCGCGCACGTGCAGGGCAA
GGTCAACGCGGGCGGCATCGGCTTCGTCGCCGCGTCCGACATCGCGATCTGCGACGAGAACGTCCCGTTCAGCCTGTCGG
AGCTGCTGTTCGGCCTGATCCCGGCGTGCGTGCTGCCGTTCCTCGCGCGCCGCATCGGCACGCCGAAAGCGCATTACATG
ACGCTGATGACGCAGCCCGTCACCGCGCAGCAGGCCTTCTCCTGGGGGCTCGTCGACGCGATCGGCGCCAATACGGACAC
GCTCGTGCGCAAGCATCTGCTGCGCCTGCGATGCCTGAACAAGGCGTCCGTCGCGCGATACAAGTCGTACGCGGCGACGC
TCGACGACACGCTCGCTGCGGCGCGCCCGGGCGCCGTGCGCGTGAGCATCGAGATGTTCGCCGATCCGGAGAACCGCCGG
AAGATCGCGCGGTACGTCGACACCGGAAAATTTCCCTGGGAGGCGGACTAA

Upstream 100 bases:

>100_bases
AAATCGACGACGGCTTCATCCCCGCGGCGCGGCGCGCGCACGGCCGCGAAACGCTCTTCCTGAGCCGGATCAACGAATAC
CATCGGGAATACGAATGGAT

Downstream 100 bases:

>100_bases
GTGAGCGCGCATGAACGAGTCGTCGAAGTCCGCGAGGTGGCGGACTGCGTAGTCCAGGTCGTCATGCAGGATCGCGAGAA
CAAGAACACGTTCTCCGACG

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: trans(or 3)-enoyl-CoA; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDTLVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD

Sequences:

>Translated_256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDTLVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD
>Mature_256_residues
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGLPDVFCFGADFSDIAEKPDAL
IDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASDIAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYM
TLMTQPVTAQQAFSWGLVDAIGANTDTLVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
KIARYVDTGKFPWEAD

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably catalyzes the dehydration of the (S)-3-hydroxy-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787659, Length=167, Percent_Identity=33.5329341317365, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17 [C]

Molecular weight: Translated: 28271; Mature: 28271

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGL
CCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PDVFCFGADFSDIAEKPDALIDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASD
CCEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECC
IAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYMTLMTQPVTAQQAFSWGLVDA
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHCCCCHHH
IGANTDTLVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
KIARYVDTGKFPWEAD
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLSRPADYRTIRVRYDGDICFLQLHRPDAQNAINNRMIAECMDVLDRCEHAAKIVVLQGL
CCCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
PDVFCFGADFSDIAEKPDALIDSDAIYGLWHRLATGPYITIAHVQGKVNAGGIGFVAASD
CCEEEECCCHHHHHCCCCHHHCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEEECC
IAICDENVPFSLSELLFGLIPACVLPFLARRIGTPKAHYMTLMTQPVTAQQAFSWGLVDA
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCCCHHHHHCCCCHHH
IGANTDTLVRKHLLRLRCLNKASVARYKSYAATLDDTLAAARPGAVRVSIEMFADPENRR
HCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHH
KIARYVDTGKFPWEAD
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-hydroxyacyl-CoA [C]

Specific reaction: (3S)-hydroxyacyl-CoA = trans(or 3)-enoyl-CoA + H2O [C]

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]