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The map label for this gene is pksI [H]

Identifier: 126445871

GI number: 126445871

Start: 1051858

End: 1052640

Strand: Reverse

Name: pksI [H]

Synonym: BMA10247_A1116

Alternate gene names: 126445871

Gene position: 1052640-1051858 (Counterclockwise)

Preceding gene: 126447145

Following gene: 126445712

Centisome position: 44.74

GC content: 65.26

Gene sequence:

>783_bases
GTGAGCGCGCATGAACGAGTCGTCGAAGTCCGCGAGGTGGCGGACTGCGTAGTCCAGGTCGTCATGCAGGATCGCGAGAA
CAAGAACACGTTCTCCGACGCGCTGATCCGGGGGCTCGGCGAGGCATTCGACGCGATCCGCCAGAACGGCCGGTGCAAGG
CCGCGATCCTCACCGGCTACGGCAACTATTTCGCGAGCGGCGGCACGCAGGAAAGCCTCATGTACCTGTCCGAGGGCAAG
GGCACGTTCGCGGAGATCGCCGCGAGCGCGAGCCGCAACGGCGCGAACCTGTACAGCCTCGCGCTGAACTGCCCCGTCCC
CGTCATTGCGGCGATGCAGGGGCACGGCATCGGCGGCGGCTTCGTGATGGGCCTGTTCGCGGACTTCGTCGTGCTCGGCA
AGGAATGCCTGTACACCACCAATTTCATGAAGTACGGCTTCACGCCGGGCATGGGCGCGACGCTCGTGCTGCCGCACAAG
CTCGGCCTGCCGCTCGCGCAGAACATGCTGATGACCGCCCGTTCGTTCTACGGCGACGAGCTGCAAAAGCTCGGTGTGCC
GTTTCCGGTGGTGCCGCGCGCGGAAGTGGCCGCGTGTGCGCTCGAGATCGCGAAGAATCTCGCCGAAAAGCCGCGGCTGT
CGCTCGTCACGCTGAAGGATCATCTGGTGGCCGAGCTGCGCTCGAGACTGCCGGCCGTGATCGAGCAGGAGCTCGCGATG
CATGAAAAGACCTTCGGCCAGCCCGAGGTCAAGGAGCGCATCCGCTCGCTCTTCGGACAATAA

Upstream 100 bases:

>100_bases
CGCCGTGCGCGTGAGCATCGAGATGTTCGCCGATCCGGAGAACCGCCGGAAGATCGCGCGGTACGTCGACACCGGAAAAT
TTCCCTGGGAGGCGGACTAA

Downstream 100 bases:

>100_bases
GTCATCCAACCCGATCGCCGCCCGTCGGCGACACCCGTCAAGGAAGCGCTGCCATGGACCTGCAGCAGGTATTCGACATT
ATCGTTCGGCATACGAAAGA

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSAHERVVEVREVADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGK
GTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHK
LGLPLAQNMLMTARSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAM
HEKTFGQPEVKERIRSLFGQ

Sequences:

>Translated_260_residues
MSAHERVVEVREVADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGK
GTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHK
LGLPLAQNMLMTARSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAM
HEKTFGQPEVKERIRSLFGQ
>Mature_259_residues
SAHERVVEVREVADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGKG
TFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKL
GLPLAQNMLMTARSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMH
EKTFGQPEVKERIRSLFGQ

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=224, Percent_Identity=23.2142857142857, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787659, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24653139, Length=184, Percent_Identity=28.804347826087, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 28187; Mature: 28056

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHERVVEVREVADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGY
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GNYFASGGTQESLMYLSEGKGTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGG
CHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCHH
FVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMTARSFYGDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHHHH
LQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAM
HHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HEKTFGQPEVKERIRSLFGQ
HHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAHERVVEVREVADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGY
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
GNYFASGGTQESLMYLSEGKGTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGG
CHHHCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCHH
FVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMTARSFYGDE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHHHH
LQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAM
HHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
HEKTFGQPEVKERIRSLFGQ
HHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]