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The map label for this gene is argT [H]

Identifier: 126445539

GI number: 126445539

Start: 430894

End: 431676

Strand: Reverse

Name: argT [H]

Synonym: BURPS668_A0474

Alternate gene names: 126445539

Gene position: 431676-430894 (Counterclockwise)

Preceding gene: 126443064

Following gene: 126445374

Centisome position: 13.8

GC content: 63.22

Gene sequence:

>783_bases
GTGAAAAAAATCTTGGCGGCTTTGACCGTGGCCCTGCTGGCGGTGTCGGCAGGCGGCGTGCAGGCGAAGGATTGGTCGAC
GATCCGCTTTGGCGTCGACGCGAGCTACCCGCCTTTCGAATCGAAAGGTCCGGACGGCAAGGTGGTCGGCTTCGACGTCG
ACCTCGGCAACGAAATCTGCAAGCGCGTGAAGGCAAAGTGCGTGTGGATCGAGAACGACTTCGACGGCATGATCCCGGCG
CTGAAGGCGCGCAAGTTCGACGGCGTGCTGTCGTCGATGTCGATGACGCCGCAGCGTGAAGAGCAGATCGCGTTCTCGTC
GAAGCTGTTCAACACGCCGACGCGCCTCGTCACGAAGAAGGGCGCGAACCTGATGCCGACGGCCGAATCGCTCAAGGGCA
AGACGGTCGGCGTCGAGCAGGGCACGATCCAGGAAACCTACGCGAAGGTGCACTGGGCGCCGATGGGCGTGAAGGTGCAA
CCGTACCAGAACCAGGACCAGGTCTACGCCGACCTGATCGCGGGCCGCCTCGACGCGGCGCTGCAGGACGCGGTGCAGGC
CGACATCGGCTTCCTGAAGACGCCGCGCGGCAAGGACTACGCGTTCGCCGGCACCGACCTCAACGATCCGAAGACGCTCG
GCGAAGGCGCGGGCATCGGCCTGCGCAAGGAAGACACCGATCTGAAGGCGAAGATCGACAAGGCGATCGCCGACATGCAC
AAGGACGGCACGTACGACAAGATCGCGAAGAAGTACTTCGACTTCGACGTCTACGGCAAGTAA

Upstream 100 bases:

>100_bases
ACCGGACGAGGGCACCAGTCAGCCCGGCCGCAGCCGCGCCGGGCCGAAGCGCACTACGGCACGACCGCGCGCAACCATCG
AACTACAACGAGGAATGACA

Downstream 100 bases:

>100_bases
CGGCAAGCCACGCCGGACCGCGGGCGACACGTCCATGCGGCGCGCCGCGACCACGGCGGAAACGGGCTTCGCTTCGCGGC
GAAGCCCGTTTTTTCATGCG

Product: putative lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKILAALTVALLAVSAGGVQAKDWSTIRFGVDASYPPFESKGPDGKVVGFDVDLGNEICKRVKAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPQREEQIAFSSKLFNTPTRLVTKKGANLMPTAESLKGKTVGVEQGTIQETYAKVHWAPMGVKVQ
PYQNQDQVYADLIAGRLDAALQDAVQADIGFLKTPRGKDYAFAGTDLNDPKTLGEGAGIGLRKEDTDLKAKIDKAIADMH
KDGTYDKIAKKYFDFDVYGK

Sequences:

>Translated_260_residues
MKKILAALTVALLAVSAGGVQAKDWSTIRFGVDASYPPFESKGPDGKVVGFDVDLGNEICKRVKAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPQREEQIAFSSKLFNTPTRLVTKKGANLMPTAESLKGKTVGVEQGTIQETYAKVHWAPMGVKVQ
PYQNQDQVYADLIAGRLDAALQDAVQADIGFLKTPRGKDYAFAGTDLNDPKTLGEGAGIGLRKEDTDLKAKIDKAIADMH
KDGTYDKIAKKYFDFDVYGK
>Mature_260_residues
MKKILAALTVALLAVSAGGVQAKDWSTIRFGVDASYPPFESKGPDGKVVGFDVDLGNEICKRVKAKCVWIENDFDGMIPA
LKARKFDGVLSSMSMTPQREEQIAFSSKLFNTPTRLVTKKGANLMPTAESLKGKTVGVEQGTIQETYAKVHWAPMGVKVQ
PYQNQDQVYADLIAGRLDAALQDAVQADIGFLKTPRGKDYAFAGTDLNDPKTLGEGAGIGLRKEDTDLKAKIDKAIADMH
KDGTYDKIAKKYFDFDVYGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=53.6679536679537, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=54.9356223175966, Blast_Score=287, Evalue=6e-79,
Organism=Escherichia coli, GI1787085, Length=254, Percent_Identity=36.2204724409449, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=33.9920948616601, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1788228, Length=255, Percent_Identity=30.1960784313725, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=32.4110671936759, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786876, Length=235, Percent_Identity=28.5106382978723, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28334; Mature: 28334

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAALTVALLAVSAGGVQAKDWSTIRFGVDASYPPFESKGPDGKVVGFDVDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
KRVKAKCVWIENDFDGMIPALKARKFDGVLSSMSMTPQREEQIAFSSKLFNTPTRLVTKK
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHC
GANLMPTAESLKGKTVGVEQGTIQETYAKVHWAPMGVKVQPYQNQDQVYADLIAGRLDAA
CCCCCCCHHHHCCCEECCCCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHHHHHH
LQDAVQADIGFLKTPRGKDYAFAGTDLNDPKTLGEGAGIGLRKEDTDLKAKIDKAIADMH
HHHHHHHHHCCEECCCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKILAALTVALLAVSAGGVQAKDWSTIRFGVDASYPPFESKGPDGKVVGFDVDLGNEIC
CHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHH
KRVKAKCVWIENDFDGMIPALKARKFDGVLSSMSMTPQREEQIAFSSKLFNTPTRLVTKK
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHC
GANLMPTAESLKGKTVGVEQGTIQETYAKVHWAPMGVKVQPYQNQDQVYADLIAGRLDAA
CCCCCCCHHHHCCCEECCCCCHHHHHHHHEEECCCCEEECCCCCHHHHHHHHHHHHHHHH
LQDAVQADIGFLKTPRGKDYAFAGTDLNDPKTLGEGAGIGLRKEDTDLKAKIDKAIADMH
HHHHHHHHHCCEECCCCCCEEEECCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KDGTYDKIAKKYFDFDVYGK
CCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]