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The map label for this gene is ampD [H]

Identifier: 126207800

GI number: 126207800

Start: 353304

End: 353852

Strand: Reverse

Name: ampD [H]

Synonym: APL_0316

Alternate gene names: 126207800

Gene position: 353852-353304 (Counterclockwise)

Preceding gene: 126207804

Following gene: 126207796

Centisome position: 15.56

GC content: 41.71

Gene sequence:

>549_bases
ATGGATCTCACGATACAAAACGGCATACTTATTCATCAAAAACAAGTTCCTTCTCCTCACTTTAACGCTCGCCCGATAGC
GAACGATATTTCACTGTTAGTGATTCACTATATCAGCCTGCCTCCGGAACAGTTCGGCGGTAATTTTATCGACCAATTTT
TCCAAGGTAATTTAGATCCGACCGTTCATCCTTATTTTGAAGAAATCAAAGACTTACGCGTTTCGGCACACTGCTTAATT
AACCGTAAAGGCGAAATTACTCAATATGTCAATTTTAACGATATGGCATGGCACGCCGGACTTTCCGAATTTAACGGGCG
AGATAAATGTAATGAATATTCGATTGGGATTGAGCTGGAAGGCAGTAATCATCAGCCGTTTACCGAAGCGCAATACCAAT
CCTTGGCGCACTTAACTCAAGCACTGATGACGGAATATCCTGAAATCACCTTACAACGTATCGCCGGACATTGCGATATT
GCACCGGAACGTAAAATCGATCCCGGACAATATTTCGATTGGGGTTATTATAAAACACTGATTGCTTAA

Upstream 100 bases:

>100_bases
ATATGTTATGAATTGTGATTTTAACACGGAACAAAGCGGAAAACACGGAAAATAGCATAAGTTATCATTCACCGGCTCAA
TAGAAACAGAAAGGAAATAT

Downstream 100 bases:

>100_bases
CGATTTAGCTCGTTTGGCATAAAAAACGGCCAGGAATAAATCCTAGCCAATATATTAGGAATGAATAAAATTTTCTAAGT
CATTTGGATGATGACCCGTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MDLTIQNGILIHQKQVPSPHFNARPIANDISLLVIHYISLPPEQFGGNFIDQFFQGNLDPTVHPYFEEIKDLRVSAHCLI
NRKGEITQYVNFNDMAWHAGLSEFNGRDKCNEYSIGIELEGSNHQPFTEAQYQSLAHLTQALMTEYPEITLQRIAGHCDI
APERKIDPGQYFDWGYYKTLIA

Sequences:

>Translated_182_residues
MDLTIQNGILIHQKQVPSPHFNARPIANDISLLVIHYISLPPEQFGGNFIDQFFQGNLDPTVHPYFEEIKDLRVSAHCLI
NRKGEITQYVNFNDMAWHAGLSEFNGRDKCNEYSIGIELEGSNHQPFTEAQYQSLAHLTQALMTEYPEITLQRIAGHCDI
APERKIDPGQYFDWGYYKTLIA
>Mature_182_residues
MDLTIQNGILIHQKQVPSPHFNARPIANDISLLVIHYISLPPEQFGGNFIDQFFQGNLDPTVHPYFEEIKDLRVSAHCLI
NRKGEITQYVNFNDMAWHAGLSEFNGRDKCNEYSIGIELEGSNHQPFTEAQYQSLAHLTQALMTEYPEITLQRIAGHCDI
APERKIDPGQYFDWGYYKTLIA

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=180, Percent_Identity=53.3333333333333, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI1787092, Length=122, Percent_Identity=38.5245901639344, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20866; Mature: 20866

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTIQNGILIHQKQVPSPHFNARPIANDISLLVIHYISLPPEQFGGNFIDQFFQGNLDP
CCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCC
TVHPYFEEIKDLRVSAHCLINRKGEITQYVNFNDMAWHAGLSEFNGRDKCNEYSIGIELE
CCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHCCHHHCCCCCCCCEEEEEEEEE
GSNHQPFTEAQYQSLAHLTQALMTEYPEITLQRIAGHCDIAPERKIDPGQYFDWGYYKTL
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IA
CC
>Mature Secondary Structure
MDLTIQNGILIHQKQVPSPHFNARPIANDISLLVIHYISLPPEQFGGNFIDQFFQGNLDP
CCEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHCCCCCC
TVHPYFEEIKDLRVSAHCLINRKGEITQYVNFNDMAWHAGLSEFNGRDKCNEYSIGIELE
CCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHCCHHHCCCCCCCCEEEEEEEEE
GSNHQPFTEAQYQSLAHLTQALMTEYPEITLQRIAGHCDIAPERKIDPGQYFDWGYYKTL
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]